RT Journal Article SR Electronic(1) A1 Lagunas, Rosario A1 Ruiz, ElviraYR 1988 T1 Balance of Production and Consumption of ATP in Ammonium-starved Saccharomyces cerevisiae JF Microbiology, VO 134 IS 9 SP 2507 OP 2511 DO https://doi.org/10.1099/00221287-134-9-2507 PB Microbiology Society, SN 1465-2080, AB To establish a balance between the ATP produced in catabolism and the ATP consumed in net biosynthesis of cellular components the energy metabolism of Saccharomyces cerevisiae utilizing glucose in the absence of a nitrogen source (resting cells) was studied. The following results were obtained. (i) Cell number and biomass increased 2- and 2ยท5-fold, respectively, during the first 8 h of ammonium starvation. After this period, both values remained constant. (ii) The rate of sugar consumption and ATP production decreased with the duration of starvation to about 20% of the original in 24 h. (iii) About 60% of the sugar consumed was fermented to ethanol and about 10% assimilated as cellular material. Of the assimilated sugar, as much as 80% was accumulated as carbohydrate. (iv) Only 15% of the total ATP produced in catabolism seems to be consumed in net biosynthesis and maintenance of intracellular pH. The fate of the remaining 85% is unknown., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-134-9-2507