%0 Journal Article %A Dickinson, J. Richard %A Smith, Maxine E. %A Swanson, Timothy R. %A Williams, Anthony S. %A Wingfield, Jonathan M. %T The cdc30 Mutation in Saccharomyces cerevisiae Affects Phosphoglucose Isomerase, the Cell Cycle and Sporulation %D 1988 %J Microbiology, %V 134 %N 9 %P 2475-2480 %@ 1465-2080 %R https://doi.org/10.1099/00221287-134-9-2475 %I Microbiology Society, %X Spontaneous revertants of the cdc30 mutation in Saccharomyces cerevisiae simultaneously regained the ability to grow and divide at 36·5 °C on glucose-containing media along with a more thermostable phosphoglucose isomerase (PGI). An independently isolated allele of cdc30 gave a similar phenotype to that previously described including temperature-sensitivity of PGI. Isoelectric focussing allowed the separation of two isoenzymes of PGI. These results all support the idea that two genes - PGI1 and CDC30 - are responsible for PGI activity in yeast. Diploid strains homozygous for the cdc30 mutation sporulated poorly in potassium acetate irrespective of whether the cells had previously been cultured at a temperature that was permissive or restrictive for cell cycle progression. This was not surprising because a strain defective in PGI would not be expected to be able to complete the gluconeogenic events of sporulation. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-134-9-2475