1887

Abstract

Spontaneous revertants of the mutation in simultaneously regained the ability to grow and divide at 36·5 °C on glucose-containing media along with a more thermostable phosphoglucose isomerase (PGI). An independently isolated allele of gave a similar phenotype to that previously described including temperature-sensitivity of PGI. Isoelectric focussing allowed the separation of two isoenzymes of PGI. These results all support the idea that two genes - and - are responsible for PGI activity in yeast. Diploid strains homozygous for the mutation sporulated poorly in potassium acetate irrespective of whether the cells had previously been cultured at a temperature that was permissive or restrictive for cell cycle progression. This was not surprising because a strain defective in PGI would not be expected to be able to complete the gluconeogenic events of sporulation.

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1988-09-01
2022-01-19
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