@article{mbs:/content/journal/micro/10.1099/00221287-134-8-2405, author = "Zvillich, Menachem and Kol, Rina and Riklis, Emanuel and Sarov, Israel", title = "Induction of DNA Strand Scissions in HeLa Cells by Human Polymorphonuclear Leucocytes Activated by Chlamydia trachomatis Elementary Bodies", journal= "Microbiology", year = "1988", volume = "134", number = "8", pages = "2405-2412", doi = "https://doi.org/10.1099/00221287-134-8-2405", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-134-8-2405", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = " Incubation of human polymorphonuclear leucocytes (HPMN) with Chlamydia trachomatis elementary bodies (EB) or phorbol 12-myristate 13-acetate (PMA) resulted in the production of superoxide anions (O− 2) and hydrogen peroxide (H2O2). Exposure of HeLa cells to EB- or PMA- activated HPMN and to EB alone, for 2h, resulted in the formation of DNA strand scissions (nicks) in the HeLa cells. The nicks were visualized by incorporation of biotin 11-dUTP with its detection by streptavidin-peroxidase, and quantified by using [3H]dCTP in the in situ nuclear nick-translation reaction. Catalase, and to a lesser extent superoxide dismutase, reduced the amount of nicks induced by the EB- or PMA-activated HPMN. The possible relationship between the activity of PMN in chlamydial infections and the development of chronic diseases is discussed.", }