1887

Abstract

Zymolyase released between 20 and 25% of the total protein from purified walls of yeast (Y) and mycelial (M) cells of . The material released contained 92% carbohydrate (86% mannose and 6% glucose) and 7:< protein. Over 85% of the carbohydrate was -glycosidically linked to the protein and the rest (less than 15%) was linked -glycosidically. Highly polydisperse, high molecular mass mannoproteins, resolved by electrophoresis as four defined bands in Y cells and two bands in M cells, had both types of sugar chains. A 34 kDa species found in both types of cells had a single 2·5 kDa -glycosidically linked sugar chain and a 3 1·5 kDa protein moiety. Polydispersity in the high molecular mass mannoproteins was due to the -linked sugar chains (mannan) with a molecular mass between 500 kDa and 20 kDa (average 100 kDa) in Y cells and between 400 kDa and 20 kDa (average 50 kDa) in M cells. Three mannoproteins of 34,30 and 29 kDa secreted by protoplasts were associated with the high molecular mass mannoproteins, suggesting that this type of interaction might be related to the regeneration of the cell wall.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-8-2393
1988-08-01
2021-05-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/8/mic-134-8-2393.html?itemId=/content/journal/micro/10.1099/00221287-134-8-2393&mimeType=html&fmt=ahah

References

  1. Ahrens J. C., Daneo-Moore L., Buckley H. R., Cutler J. E. 1983; Differential protein synthesis in Candida structural and biochemical studies of two dynami- albicans during blastospore formation at 24-5 °C and during germ tube formation at 37 °C. General Microbilogy 129:1113–1339
    [Google Scholar]
  2. Brawner D. J., Cutler J. E. 1986; Ultra-cally expressed cell surface determinants on during structural and biochemical studies of two dynamially expressed cell surface determinants on Candaia albicans . Infection and Immunity 51:327–336
    [Google Scholar]
  3. Chattaway F. W., Holmes M. R., Barlow A. J. E. 1968; Cell wall composition of the mycelial and blastospore forms of Candida albicans . Journal of General Microbiology 51:367–376
    [Google Scholar]
  4. Chu F. K., Watorek W., Maley F. 1983; Factors affecting the oligomeric structure of yeast external invertase. Archives of Biochemistry and Biophysics 223:543–555
    [Google Scholar]
  5. Dubois M., Gilles K., Hamilton J., Rebers P., Smith F. 1956; Colorimetric method for determination of sugar and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  6. Elorza M. V., Rico H., Sentandreu R. 1983; Calcofluor white alters the assembly of chitin fibrils in Sacchpromyces cerevisiae and Candida albicans cells. Journal of General Microbiology 129:1577–1582
    [Google Scholar]
  7. Elorza M. V., Murgui A., Sentandreu R. 1985; Dimorphism in Candida albicans: contribution of mannoproteins to the architecture of yeast and mycelial cell walls. Journal of General Microbiology 131:2209–2216
    [Google Scholar]
  8. Elorza M. V., Murgui A., Rico H., Miragall F., Sentandreu R. 1987; Formation of new cell wall by protoplasts of Candida albicans: effect of papulacandin B, tunicamycin and nikkomycin. Journal of General Microbiology 133:2315–2325
    [Google Scholar]
  9. Esmon P. C., Esmon B. E., Schauer I. E., Taylor A., Schekman R. 1987; Structure, assembly, and secretion of octameric invertase. Journal of Biological Chemistry 262:4387–4394
    [Google Scholar]
  10. Farkaš J., Kovarik A., Kosinova A., Bauer S. 1974; Autoradiographic study of mannan incorporation into growing cell walls of Saccharomyces cerevisiae . Journal of Bacteriology 117:265–269
    [Google Scholar]
  11. Frevert J., Ballou C. E. 1985; Saccharomyces cerevisiae structural cell wall mannoproteins. Biochemistry 24:753–759
    [Google Scholar]
  12. Gascon S., Lampen J. O. 1968; Purification of the internal invertase of yeast. Journal of Biological Chemistry 243:1567–1572
    [Google Scholar]
  13. Haworth W. N., Heat R. L., Peat S. 1941; The constitution of yeast mannan. Journal of the Chemical Society833–842
    [Google Scholar]
  14. Herrero E., Pastor J., Sentandreu R. 1982; Turnover of protein components of the plasma membrane of Saccharomyces cerevisiae . Biochimica et biophysica acta 689:38–44
    [Google Scholar]
  15. Herrero E., Sanz P., Sentandreu R. 1987; Cell wall proteins liberated by Zymolyase from several ascomycetous and imperfect yeasts. Journal of General Microbiology 133:2895–2903
    [Google Scholar]
  16. Kolarova N., Masler L., Sikl D. 1973; Cell wall glycopeptides of Candida albicans serotypes A and B. Biochimica et biophysica acta 428:221–227
    [Google Scholar]
  17. Lee C. J., King R. D. 1983; Characterization of Candida albicans adherence to human vaginal epithelial cells in vitro . Infection and Immunity 41:1024–1030
    [Google Scholar]
  18. Lee K., Buckley H. R., Campbell C. C. 1975; An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida albicans . Sabouraudia 13:148–153
    [Google Scholar]
  19. Lloyd J., Whelan W. 1969; An improved method of enzymic determination of glucose in the presence of maltose. Analytical Biochemistry 30:467–470
    [Google Scholar]
  20. Matzuc M. M., Krieger M., Corless C. L., Boime I. 1987; Effects of preventing O-glyco- sylation on the secretion of human choryonic gonadotrophin in Chinese hamster ovary cells. Proceedings of the National Academy of Sciences of the United States of America 84:6354–6358
    [Google Scholar]
  21. Millette C. F., Scott B. K. 1984; Identification of spermatogenic cell plasma membrane glycoprotein by two dimensional electrophoresis and lectin blotting. Journal of Cell Science 65:233–248
    [Google Scholar]
  22. Murgui A., Elorza M. V., Sentandreu R. 1985; Effect of papulacandin A and calcofluor white on the incorporation of mannoproteins in the cell wall of Candida albicans blastospores . Biochimica et biophysica acta 841:215–222
    [Google Scholar]
  23. Murgui A., Elorza M. V., Sentandreu R. 1986; Tunicamycin and papulacandin A inhibit incorporation of specific mannoproteins into the walls of Candida albicans regenerating protoplasts. Biochimica et biophysica acta 884:550–558
    [Google Scholar]
  24. Niimi M., Niimi K., Tokunaga J., Nakayama H. 1980; Changes in cyclic nucleotide levels and dimorphic transition in Candida albicans . Journal of Bacteriology 142:1010–1014
    [Google Scholar]
  25. Pastor F. I. J., Valentin E., Herrero E., Sentandreu R. 1984; Structure of Saccharomyces cerevisiae cell wall mannoproteins released by Zymolyase and their contribution to wall architecture. Biochimica et biophysica acta 802:292–300
    [Google Scholar]
  26. Ponton J., Jones J. M. 1986; Identification of two germ-tube-specific cell wall antigens of Candida albicans . Infection and Immunity 54:864–868
    [Google Scholar]
  27. Sentandreu R., Northcote D. H. 1968; The structure of a glycopeptide isolated from the yeast cell wall. Biochemical Journal 109:419–432
    [Google Scholar]
  28. Shibata N., Kabayashi H., Tojo M., Suzuki S. 1986; Characterization of phosphomannan-protein complexes isolated from viable cells of yeast and mycelial forms of Candida albicans NIH B-792 strain by the action of Zymolyase-100T. Archives of Biochemistry and Biophysics 251:697–708
    [Google Scholar]
  29. Smith C. B. 1985; Candidiasis: pathogenesis, host resistance, and predisposing factors. In Candidiasis pp. 53–70 Bodey G. P., Fainstein V. Edited by New York: Raven Press;
    [Google Scholar]
  30. Sobel J. D., Muller G., Buckley H. R. 1984; Critical role of germ tube formation in the pathogenesis of candidal vaginitis. Infection and Immunity 44:576–580
    [Google Scholar]
  31. Stanley P. 1987; Glycosylation mutants and the functions of mammalian carbohydrates. Trends in Genetics 3:77–81
    [Google Scholar]
  32. Sullivan P. A., Chiew Y. Y., Molloy C., Templeton M. D., Shepherd M. G. 1983; An analysis of the metabolism and cell wall composition of Candida albicans during germ-tube formation. Canadian Journal of Microbiology 29:1514–1525
    [Google Scholar]
  33. Sundstrom P. M., Kenny G. E. 1984; Characterization of antigens specific to the surface of germ tubes of Candida albicans by immunofluorescence. Infection and Immunity 43:850–855
    [Google Scholar]
  34. Sundstrom P. M., Nichols E. J., Kenny G. E. 1987; Antigenic differences between mannoproteins of germ tubes and blastospores of Candida albicans . Infection and Immunity 55:616–620
    [Google Scholar]
  35. Tkacz J. 1984; In vivo synthesis of β1,6-glucan in Saccharomyces cerevisiae . In Microbial Cell Wall Synthesis and Autolysis pp. 287–295 Nombela C. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  36. Tammi M., Ballou L., Taylor A., Ballou C. 1987; Effect of glycosylation on yeast invertase oligomer stability. Journal of Biological Chemistry 262:4395–4401
    [Google Scholar]
  37. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  38. Valentin E., Herrero E., Rico H., Miragall F., Sentandreu R. 1987; Cell wall mannoproteins during the population growth phases in Saccharomyces cerevisiae . Archives of Microbiology 148:88–94
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-8-2393
Loading
/content/journal/micro/10.1099/00221287-134-8-2393
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error