1887

Abstract

Intracellular concentrations of acetyl-CoA and malonyl-CoA in K12 were determined by a malonyl-CoA:acetyl-CoA cycling technique. Under aerobic growth conditions with glucose the acetyl-CoA and malonyl-CoA concentrations varied over a range of 0.05-1.5 nmol (mg dry wt) (20-600 μM) and 0·01-0·23 mol (mg dry wt) (4·90 μ), respectively. The intracellular concentration of acetyl-CoA was highest in exponentially growing cells and it fell rapidly to less than 5% of the maximum level when the organism entered stationary phase after exhaustion of glucose. A linear relationship was observed between the intracellular concentration of total acyl-CoA and the logarithm of the concentration of glucose in the medium. Consequently, the acetyl-CoA/malonyl-CoA ratios also varied drastically, in a range of 0·6-41·7, under different conditions. Of several carbon sources tested, glucose was the most effective for promoting the synthesis of cellular acetyl-CoA. For cells grown on glycerol or acetate the maximum concentrations of total acyl-CoA were significantly lower. In cells incubated with citrate (not used as a carbon source by ), the level was consistent with that in cells starved for exogenous carbon sources.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-8-2249
1988-08-01
2021-05-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/8/mic-134-8-2249.html?itemId=/content/journal/micro/10.1099/00221287-134-8-2249&mimeType=html&fmt=ahah

References

  1. Bockner B.R., Ames B.N. 1982; Complete analysis of cellular nucleotides by two dimensional thin layer chromatography.. Journal of Biological Chemistry 257:9759–9769
    [Google Scholar]
  2. Canovas J.K., Kornberg H.L. 1965; Fine control of phosphoenolpyruvate carboxylase activity in Escherichia coli.. Biochimica et biophysica acta 96:169–172
    [Google Scholar]
  3. Cazzulo J.J., Stoppanni A.O.M. 1967; Purification and properties of pyruvate carboxylase from baker’s yeast.. Archives of Biochemistry and Biophysics 121:596–608
    [Google Scholar]
  4. Chapman A.G., Fall I., Atkinson D.E. 1971; Adenylate energy charge in Escherichia coli during growth and starvation.. Journal of Bacteriology 108:1072–1086
    [Google Scholar]
  5. Cooper T.G., Benedict C.R. 1968; Regulation of pyruvate carboxylase by coenzyme A and acyl-coenzyme A thioesters.. Biochemistry 7:3032–3036
    [Google Scholar]
  6. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. 1956; A colorimetric method for determination of sugars.. Analytical Chemistry 28:350–356
    [Google Scholar]
  7. Hansen R.G., Henning U. 1966; Regulation of pyruvate dehydrogenase activity in, Escherichia coli K-12.. Biochimica et biophysica acta 12:355–358
    [Google Scholar]
  8. Jackowski S., Rock C.O. 1986; Consequences of reduced intracellular coenzyme A content in Escherichia coli.. Journal of Bacteriology 166:866–871
    [Google Scholar]
  9. Kawaguchi A., Arai K., Seyama Y., Yamakawa T., Okuda S. 1980; Substrate control of termination of fatty acid synthesis by fatty acid synthetase from Brevibacterium ammoniagenes.. Journal of Biochemistry 88:303–306
    [Google Scholar]
  10. Nomura G., Yoshida Y., Takamura Y. 1988; Measurement of the intracellular concentrations of acetyl-CoA and malonyl-CoA in bacteria and yeasts using acyl-CoA cycling.. Agricultural and Biological Chemistry 52:843–844
    [Google Scholar]
  11. Okuyama H., Saitoh M., Hiramatsu R. 1982; Fatty acid synthetase system in the regulation of membrane lipid synthesis in Escherichia coli after shift in temperature.. Journal of Biological Chemistry 257:4812–4817
    [Google Scholar]
  12. Sanwal B.D., Maeba P. 1966; Regulation of the activity of phosphoenolpyruvate carboxylase by fructose diphosphate.. Biochimica et biophysica acta 22:194–199
    [Google Scholar]
  13. Schwartz E.R, Old L.O., Reed L.J. 1968; Regulatory properties of pyruvate dehydrogenase from Escherichia coli.. Biochemical and Biophysical Research Communications 31:495–500
    [Google Scholar]
  14. Shen L.C., Fall L., Walton G.M., Atkinson D.E. 1968; Interaction between energy charge and metabolic modulation in the regulation of enzymes of amphibolic sequences. Phosphofructokinase and pyruvate dehydrogenase.. Biochemistry 7:4041–4045
    [Google Scholar]
  15. Sugimoto S., Shiio I. 1987; Regulation of 6-phosphogluconate dehydrogenase in Brevibacterium flavum.. Agricultural and Biological Chemistry 51:1257–1263
    [Google Scholar]
  16. Swedes J.S., Sedo R.T., Atkinson D.E. 1975; Relation of growth and protein synthesis to the adenylate energy charge in adenine-requiring mutant of Escherichia coli.. Journal of Biological Chemistry 250:6930–6938
    [Google Scholar]
  17. Takamura Y., Kitayama Y. 1981; Purification and some properties of malonate decarboxylase.. Biochemistry International 3:483–491
    [Google Scholar]
  18. Takamura Y., Kitayama Y., Arakawa A., Yaman-AKA S., Tosaki M., Ogawa Y. 1985; Malonyl-CoA: acetyl-CoA cycling. A new micromethod for determination of acyl-CoAs with malonate decarboxylase.. Biochimica et biophysica acta 83:1–7
    [Google Scholar]
  19. Utter M.F., Kohlenbrander M. 1972; Formation of oxaloacetate by CO2 fixation on phosphoenolpyruvate.. In The Enzymes 6: pp 117–168 Boyer P. D. Edited by New York: Academic Press;
    [Google Scholar]
  20. Vallari D.S., Jackowski S., Rock C.O. 1987; Regulation of pantothenate kinase by coenzyme A and its thioesters.. Journal of Biological Chemistry 262:2468–2471
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-8-2249
Loading
/content/journal/micro/10.1099/00221287-134-8-2249
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error