1887

Abstract

A cytochemical staining method was developed for the demonstration of hydrogenase activity in hydrogenosomes of . After fixation in low concentrations of glutaralde-hyde under strictly anaerobic conditions and incubation of these cells in the presence of H and the tetrazolium compound 2-(2′-benzothiazolyl)-5-styryl-3-(4′-phthalhydrazidyl)-tetrazolium chloride, an electron-dense deposit was produced in the hydrogenosomes. The method was used to demonstrate the hydrogenosomal nature of similar microbodies of the sapropelic ciliates and .

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/content/journal/micro/10.1099/00221287-134-8-2165
1988-08-01
2021-10-22
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