1887

Abstract

A central feature of the processes of aggregation and differentiation in the cellular slime mould is the periodic excitatory cycle. Originally thought to involve primarily fluctuations in cyclic AMP levels, this excitatory cycle has since been shown to involve changes in several other second messengers including cyclic G MP, calcium and inositol trisphosphate. Previous work from this laboratory using specific inhibitors strongly suggested a role for eicosanoids in this stimulus-response process. Production of eicosanoids from fatty acid precursors is an oxygen-consuming process. In this paper, we report on oxygen consumption measurements in intact cells and in cell extracts. We demonstrate the existence of an azide-insensitive component of oxygen consumption which can be stimulated by the addition of arachidonate and other polyunsaturated fatty acids, and at least partially inhibited by meclofenamate and eicosatetraynoic acid, both of which block eicosanoid biosynthesis in higher organisms. These observations provide further evidence for the existence of an eicosanoid-metabolizing system in .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-8-2123
1988-08-01
2021-08-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/8/mic-134-8-2123.html?itemId=/content/journal/micro/10.1099/00221287-134-8-2123&mimeType=html&fmt=ahah

References

  1. Chung W. K., Coe E. L. 1978; Correlations among the responses of suspensions of Dictyostelium discoideumto pulses of 3ʹ,5ʹ-cyclic AMP. Biochimica et biophysica acta 544:29–44
    [Google Scholar]
  2. Dohrmann V., Fisher P. R., Bruderlein M., Luchner B., Williams K. L. 1984; Arachidonic acid and related molecules affect the behaviour of Dictyostelium discoideumslugs. Journal of General Microbiology 130:2685–2698
    [Google Scholar]
  3. Eling T., Tainer B., Ally A., Warnock R. 1982; Separation of arachidonic acid metabolites by high pressure liquid chromatography. Methods in Enzymology 86:511–517
    [Google Scholar]
  4. Jakschik B. A., Harper T., Murphy R. C. 1982; The 5-lipoxygenase and leukotriene forming enzymes. Methods in Enzymology 86:30–37
    [Google Scholar]
  5. Loomis W. F. 1982 The Development of Dictyostelium discoideum. p. 235 New York: Academic Press;
    [Google Scholar]
  6. Mcphail L. C., Clayton C. C., Snyderman R. 1984; A potential second messenger role for unsaturated fatty acids: activation of Ca2+-depen- dent protein kinase. Science 224:622–625
    [Google Scholar]
  7. Newell P. C., Europe-Finner G. N., Small N. V. 1987; Signal transduction during amoebal chemotaxis of Dictyostelium discoideum. . Microbiological Sciences 4:5–11
    [Google Scholar]
  8. Sampson J., Town C. D. 1985; Suppression of cyclic AMP induced cell excitation in Dictyostelium discoideumby inhibitors of eicosanoid oxidation. FEMS Microbiology Letters 27:209–213
    [Google Scholar]
  9. Schonbaum G. R., Bonner W. D. JR. Storey B. T. 1971; Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids. Plant Physiology 47:124–128
    [Google Scholar]
  10. Town C. D., Krill D., Hassid A. I. 1985; Inhibition of differentiation in Dictyostellium discoideumby anti-inflammatory drugs. Development, Growth and Differentiation 27:111–116
    [Google Scholar]
  11. Umbreit W. W., Burns R. H., Stauffer J. F. 1972 Manometric Biochemical Techniques p. 7 Minneapolis, Minn.: Burgess Publishing Co.;
    [Google Scholar]
  12. Watts D. J., Ashworth J. M. 1970; Growth of myxamoebae of the cellular slime mold Dictyostelium discoideumin axenic culture. Biochemical Journal 119:171–174
    [Google Scholar]
  13. Weeks G. 1976; The manipulation of the fatty acid composition of Dictyostelium discoideumand its effect on cell differentiation. Biochimica et biophysica acta 450:21–32
    [Google Scholar]
  14. Woffendin C., Griffiths A. J. 1984; The effect of respiratory inhibitors during growth and development of Dictyostelium discoideum. . Biochimica et biophysica acta 766:542–548
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-8-2123
Loading
/content/journal/micro/10.1099/00221287-134-8-2123
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error