1887

Abstract

An model of the regenerative phase of the human endometrial cycle was developed in order to study the growth of during the period following menses. Glandular epithelial fragments were prepared from curettings of endometria and explanted onto coated substrata. Epithelial cells migrated rapidly from the explant in a fashion which closely mimicked the regeneration of the surface epithelium after menses. The cultures were then experimentally infected with serotype E at various times during formation of the outgrowth. Chlamydial inclusions developed both within the explants and in the outgrowing epithelial sheets. They were also found in isolated epithelial and non-epithelial cells. However, the most striking feature of chlamydial inclusion development within these cultures was the tendency for inclusions to be located in cells at the periphery of the epithelial sheets. This was partly due to the failure of the cells within the sheets to bind chlamydiae after centrifugation of the organisms onto the culture and partly due to a phenomenon similar to phagokinesis. During this process infectious chlamydial particles were cleared from the substratum by migrating cells with free motile edges, which occasionally led to internalization and inclusion development within these cells.

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1988-07-01
2021-05-15
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References

  1. Albrecht-Buehler G. 1977; The phagokinetic tracts of 3T3 cells. Cell 11:395–404
    [Google Scholar]
  2. Albrecht-Buehler G., Goldman R. D. 1976; Microspike-mediated particle transport towards the cell body during early spreading of 3T3 cells. Experimental Cell Research 97:329–339
    [Google Scholar]
  3. Albrecht-Buehler G., Lancaster R. 1976; A quantitative description of the extension and retraction of surface protrusions in spreading 3T3 mouse fibroblasts. Journal of Cell Biology 71:370–382
    [Google Scholar]
  4. Albrecht-Buehler G., Bushnell A. 1979; The orientation of centrioles in migrating 3T3 cells. Experimental Cell Research 120:111–118
    [Google Scholar]
  5. Allen T. D. 1987; Time lapse video microscopy using an animation control unit. Journal of Microscopy 147:129–135
    [Google Scholar]
  6. Campbell S., Allen T. D., Aplin J. D. 1984; Novel substratum-dependent dentritic morphology and motility in epithelial cells. European Journal of Cell Biology 34:275–280
    [Google Scholar]
  7. Demopoulos R. J. 1982; Normal endometrium. In Pathology of the Female Genital Tract,, 2nd edn.. pp 235–278 Blaustein A. Edited by Berlin: Springer;
    [Google Scholar]
  8. Dipasquale A. 1975a; Locomotion of epithelial cells. Experimental Cell Research 95:425–439
    [Google Scholar]
  9. Dipasquale A. 1975b; Locomotory activity of epithelial cells in culture. Experimental Cell Research 94:191–215
    [Google Scholar]
  10. Elsdale T., Bard J. 1972; Collagen substrate for studies on cell behaviour. Journal of Cell Biology 54:626–637
    [Google Scholar]
  11. Faber M., Wever U. M., Berthelsen J. G., Liotta L. A., Alrechten R. 1986; Laminin production by human endometrial stomal cells relates to the cyclic and pathologic state of the endometrium. American Journal of Pathology 124:384–391
    [Google Scholar]
  12. Ferenczy A. 1975a; Studies on the cytodynamics of human endometrial regeneration. 1. Scanning electron microscopy. American Journal of Obstetrics and Gynecology 124:64–74
    [Google Scholar]
  13. Ferenczy A. 1975b; Studies on the cytodynamics of human endometrial regeneration. II. Transmission electron microscopy and histochemistry. American Journal of Obstetrics and Gynecology 124:582–595
    [Google Scholar]
  14. Ferenczy A., Richart R. M. 1974; Scanning and transmission electron microscopy of the human endometrial surface epithelium. Journal of Clinical Endocrinology and Metabolism 36:999–1008
    [Google Scholar]
  15. Froman G., Switalski L. M., Faris , Wadstrom T., Hook M. 1984; Binding of Escherichia coli to fibronectin. Journal of Biological Chemistry 23:14899–14905
    [Google Scholar]
  16. Gump D. W., Dickstein S., Gibson M. 1981; Endometritis related to Chlamydia trachomatis infection. Annals of Internal Medicine 95:61–63
    [Google Scholar]
  17. Hafez E. S. E., Ludwig H. 1977; Scanning electron microscopy of the endometrium. In Biology of the Uterus, 2nd edn.. pp 309–340 Wynn R. M. Edited by New York: Plenum Press;
    [Google Scholar]
  18. Hafez E. S. E., Barnhart M. J., Ludwig H., Lusher J., Joelsson J., Daniel J. L., Sherman A. I., Jordan J. A., Wolf H., Stewart W. C., Chretien F. C. 1975; Scanning microscopy of human reproductive physiology. Acta obsterica et gynecologica scandinavica Supplement 40:5–61
    [Google Scholar]
  19. Hodinka R. L., Wyrick P. B. 1986; Ultrastructural study of mode of entry of Chlamydia psittaci into L-929 cells. Infection and Immunity 54:855–863
    [Google Scholar]
  20. Hopkins C. R. 1985; The appearance and internalisation of transferrin receptors at the margins of spreading human tumour cells. Cell 40:199–208
    [Google Scholar]
  21. Kirk D., King R. J. B., Heyes J., Peachy L., Hirsch P. J., Taylor R. W. 1978; Normal human endometrium in cell culture. I. Separation and characterisation of epithelial and stromal components in vitro . In Vitro 14:651–662
    [Google Scholar]
  22. Kirk D., Irwin J. C. 1980; Normal human endometrium in cell culture. Methods in Cell Biology 21B:51–77
    [Google Scholar]
  23. Lenton E. A., Sulaiman R., Sobowale O., Cooke I. D. 1982; The human menstrual cycle: plasma concentrations of prolactin, LH, FSH estradiol and progesterone in conceiving and non-conceiving women. Journal of Reproduction and Fertility 65:131–139
    [Google Scholar]
  24. Masterton R., Armstrong E. M., More I. A. R. 1975; The cyclical variation in the percentage of cilliated cells in the normal human endometrium. Journal of Reproduction and Fertility 42:537–540
    [Google Scholar]
  25. Maxe I., Ryden C., Wadstrom T., Rubin K. 1986; Specific attachment of Staphylococcus aureus to immobilised fibronectin. Infection and Immunity 54:695–704
    [Google Scholar]
  26. Mcauslan B. R., Reilly W. 1980; Endothelial cell phagokinesis in response to specific metal ions. Experimental Cell Research 130:147–157
    [Google Scholar]
  27. Moll R., Levy R., Czernobilsky B., Hohlweg-Majert P., Dallenbach-Hellweg G., Franke W. W. 1983; Cytokeratins of normal epithelia and some neoplasms of the female genital tract. Laboratory Investigation 49:599–610
    [Google Scholar]
  28. Moorman D. R., Sixbey J. W., Wyrick P. B. 1986; Interaction of Chlamydia trachomatis with human genital epithelium. Journal of General Microbiology 132:1055–1067
    [Google Scholar]
  29. Paavonen J., Aine R., Teisala K., Heinonen P. K., Punnonen R., Lehtinen M., Miettinen A., Gronroos P. 1985; Chlamydial endometritis. Journal of Clinical Pathology 38:726–732
    [Google Scholar]
  30. Pearce J. H. 1986; Early events in chlamydial infection. Annales de l’Institut Pasteur/Microbiologie 137A:307–356
    [Google Scholar]
  31. Richmond S. J. 1980; Chlamydial group antigen in McCoy cells infected with Chlamydia trachomatis and Chlamydia psittaci . FEMS Microbiology Letters 8:47–50
    [Google Scholar]
  32. Richmond S. J. 1985; Division and transmission of inclusions of Chlamydia trachomatis in replicating McCoy cell monolayers. FEMS Microbiology Letters 29:49–52
    [Google Scholar]
  33. Richmond S. J., Crosdale E., Lusher M., Haynes P. 1986; Inclusion division and pleomor- phism in cultured human endometrial cells. In Chlamydial Infections pp 63–66 Oriel D., Ridgeway D., Schachter J., Taylor-Robinson D., Ward M. Edited by London: Cambridge University Press;
    [Google Scholar]
  34. Soderlund G., Kihlstrom E. 1983; Attachment and internalisation of a Chlamydia trachomatis lymphogranuloma venereum strain by McCoy cells: kinetics of infectivity and effect of lectins and carbohydrates. Infection and Immunity 42:930–935
    [Google Scholar]
  35. Sompolinsky D., Richmond S. J. 1974; Growth of Chlamydia trachomatis in McCoy cells treated with cytochalasin B. Applied Microbiology 28:912–914
    [Google Scholar]
  36. Speziale P., Hook M., Wadstrom T. 1982; Binding of the basement membrane protein laminin to Escherichia coli . FEBS Letters 146:55–58
    [Google Scholar]
  37. Sweet R. L., Blankfort-Doyle M., Robbie M. O., Schachter J. 1986; The occurrence of chlamydial and gonococcal salpingitis during the menstrual cycle. Journal of the American Medical Association 255:2062–2064
    [Google Scholar]
  38. Switalski L. M., Ljungh A., Ryden C., Rubin K., Hook M., Wadstrom T. 1982; Binding of fibronectin to the surface of group A, C, G, streptococci isolated from human infections. European Journal of Clinical Microbiology 1:381–387
    [Google Scholar]
  39. Trent J. M., Davis J. R., Payne C. M. 1980; The establishment and morphological characterisation of finite cell lines from human endometrium. American Journal of Obstetrics and Gynecology 136:352–362
    [Google Scholar]
  40. Ward M. E., Murray A. 1984; Control mechanisms governing the infectivity of Chlamydia trachomatis for HeLa cells: mechanisms of endocytosis. Journal of General Microbiology 130:1765–1780
    [Google Scholar]
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