1887

Abstract

Regeneration of the cell wall in yeast protoplasts can be blocked by the action of snail enzymes. Electron microscopic studies of protoplasts of var. showed that incubation in the presence of snail enzymes resulted in the production of incomplete cell walls consisting of -(1 → 3)-glucan microfibrils aggregated into flat sheets of irregular size. This incomplete cell wall did not allow the formation of a septum and subsequent cell division. In contrast, protoplasts of the same yeast species growing in a flattened state produced by physical constraint formed incomplete walls that permitted cytokinesis but not reversion of the protoplasts to normal cells. These incomplete walls comprised three structural components: (i) a continuous network of long -(1 →3)-glucan microfibrils; (ii) short -(1 →3)-glucan microfibrils; and (iii) an amorphous matrix. This ultrastructural picture corresponded to the first stages of regeneration (reached after 2–3 h incubation) leading to a complete cell wall; the walls in the flattened protoplasts, however, were not completed and consequently the protoplasts did not revert to normal cells.

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1988-07-01
2021-07-28
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References

  1. Gabriel M. 1983; Karyokinesis and cell septum formation during incomplete cell wall regeneration in flattened Schizosaccharomyces versatilis protoplasts. In Proceedings of 6th European Cell Cycle Workshop Progress in Cell Cycle Controls, Prague 1983 pp. 144–146 Chaloupka J., Kotyk A., Streiblová E. Edited by Prague: Institute of Microbiology Czechoslovak Academy of Sciences;
    [Google Scholar]
  2. Gabriel M. 1984; Karyokinesis and septum formation during the regeneration of incomplete cell walls in protoplasts of Schizosaccharomyces japonicus var. versatilis: a time-lapse microcinematographic study. Journal of General Microbiology 130:625–630
    [Google Scholar]
  3. Gabriel M., Kopecká M. 1983; Regeneration of the cell wall of protoplasts of the fission yeast Schizosaccharomyces versatilis in liquid media and their reversion to cells. Folia microbiologica 28:452–457
    [Google Scholar]
  4. Havelková M. 1972; Experimental inhibition of cell wall formation and of reversion in Nadsoniaelongataand Schizosaccharomycespombe protoplasts. Protoplasma 75:405–419
    [Google Scholar]
  5. Havelková M. 1981; Multiple septation in multinucleate protoplasts of Schizosaccharomyces pombe. Zeitschrift für Allgemeine Mikrobiologie 21:261–266
    [Google Scholar]
  6. Havelková M., Křípalová J. 1980; Independence of nuclear cycle on cell wall regeneration in Schizosaccharomyces pombe protoplasts. Folia microbiologica 25:219–224
    [Google Scholar]
  7. Kopecká M., Kreger D. R. 1986; Assembly of microfibrils in vivo and in vitro from (1 →3)-β-d-glucan synthesized by protoplasts of Saccharomyces cerevisiae. Archives of Microbiology 143:387–395
    [Google Scholar]
  8. Kopecká M., Phaff H. J., Fleet G. H. 1974a; Demonstration of a fibrillar component in the cell wall of the yeast Saccharomyces cerevisiae and its chemical nature. Journal of Cell Biology 62:66–76
    [Google Scholar]
  9. Kopecká M., Phaff H. J., Fleet G. H. 1974b; The ultrastructure of the yeast cell wall after enzymic degradation by purified enzymes. In Proceedings of the Fourth International Symposium on Yeasts, Vienna, Austria, part I pp. 205–206 Klaushofer H., Sleytr U. B. Edited by Vienna: Hochschiiler- schaftan der Hochschule für Bodenkultur;
    [Google Scholar]
  10. Kreger D. R., Kopecká M. 1973; On the nature of fibrillar nets formed by protoplasts of Saccharomyces cerevisiae in liquid media. In Yeast, Mould and Plant Protoplasts pp. 117–130 Villanueva J. R., García-Acha I., Gascón S., Uruburu F. Edited by London: Academic Press;
    [Google Scholar]
  11. Kreger D. R., Kopecká M. 1976a; On the nature and formation of the fibrillar nets produced by protoplasts of Saccharomyces cerevisiae in liquid media: an electronmicroscopic X-ray diffraction and chemical study. Journal of General Microbiology 92:207–220
    [Google Scholar]
  12. Kreger D. R., Kopecká M. 1976b; Assembly of wall polymers during the regeneration of yeast protoplasts. In Microbial and Plant Protoplasts pp. 237–252 Peberdy J. F., Rose A. H., Rogers H. J., Cocking E. C. Edited by London: Academic Press;
    [Google Scholar]
  13. Kreger D. R., Kopecká M. 1978; The nature of the nets produced by protoplasts of Schizosaccharomyces pombe during the first stage of wall regeneration in liquid media. Journal of General Microbiology 108:269–274
    [Google Scholar]
  14. Nečas O. 1961; Physical conditions as important factors for the regeneration of naked yeast protoplasts. Nature; London: 192580–581
    [Google Scholar]
  15. Nečas O. 1962; The mechanism of regeneration of yeast protoplasts.Physical conditions. Folia biologica 8:256–262
    [Google Scholar]
  16. Nečas O. 1965; Mechanism of regeneration of yeast protoplasts. Folia biologica 11:371–377
    [Google Scholar]
  17. Nečas O. 1971; Cell wall synthesis in yeast protoplasts. Bacteriological Reviews 15:149–170
    [Google Scholar]
  18. Nečas O., Svoboda A. 1967; Relation between the biosynthesis of the cell wall and regeneration in yeast protoplasts. In Symposium über Hefe-Protoplasten pp. 67–71 Müller R. Edited by Berlin: Akademie-Verlag;
    [Google Scholar]
  19. Nečas O., Svoboda A. 1981; Morphogenesis in protoplasts. In Yeast Cell Envelopes: Biochemistry, Biophysics and Ultrastructure 2 pp. 105–127 Arnold W. N. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  20. Nečas O., Svoboda A. 1985; Cell wall regeneration and protoplast reversion. In Fungal Protoplasts pp. 115–133 Peberdy J. F., Ferenczy L. Edited by New York: Marcel Dekker;
    [Google Scholar]
  21. Phaff H. J. 1971; Structure and biosynthesis of the yeast cell envelope. In The Yeasts 2: pp. 135–210 Rose A. H., Harrison J. S. Edited by London & New York: Academic Press;
    [Google Scholar]
  22. Svoboda A. 1966; Regeneration of yeast protoplasts in agar gels. Experimental Cell Research 44:640–642
    [Google Scholar]
  23. Svoboda A. 1967; Regeneration ability of protoplasts of different yeast species. In Symposium über Hefe-Protoplasten pp. 31–36 Müller R. Edited by Berlin: Akademie-Verlag;
    [Google Scholar]
  24. Svoboda A., Nečas O. 1970; Experimental decoupling in the synthesis of fibrillar and amorphous components during regeneration of the wall in Saccharomyces cerevisiae protoplasts. In Yeast Protoplasts, Proceedings of the Second International Symposium on Yeast Protoplasts pp. 211–215 Nečas O., Svoboda A. Edited by Brno:: J. E. Purkyně University Press;
    [Google Scholar]
  25. De Vries O. M. H., Wessels J. G. H. 1973; Release of protoplasts from Schizophyllum commune by combined action of purified α-l,3-glucanase and chitinase derived from Trichodermaviride. Journal of General Microbiology 76:319–330
    [Google Scholar]
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