Characterization of Two Families of Spontaneously Amplifiable Units of DNA in Free

Abstract

Four highly amplified DNA sequences (ADS) ranging from 5·8 to 24·8 kb were found in spontaneous mutant strains of DSM 40697. Restriction patterns of total DNA were hybridized with purified ADS6 (24·8 kb) as a probe to detect the amplifiable regions in the wild-type (WT) genome. The results suggested that the amplifiable unit of DNA (AUD) was present as a single copy in the WT genome. Moreover, similarities suggested by the restriction maps of three of the ADS were confirmed by hybridization experiments. The fourth ADS did not hybridize with the three others. Therefore, two families of DNA sequences are potentially amplifiable in the genome.

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1988-07-01
2024-03-29
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References

  1. Altenbüchner J., Cullum J. 1984; DNA amplification and an unstable arginine gene in Streptomyces lividans 66. Molecular and General Genetics 195:134–138
    [Google Scholar]
  2. Altenbüchner J., Cullum J. 1985; Structure of an amplifiable DNA sequence in Streptomyces lividans 66. Molecular and General Genetics 201:192–197
    [Google Scholar]
  3. Baltz H. R., Stonesifer J. 1985; Phenotypic changes associated with loss of expression of tylosin biosynthesis and resistance genes in Streptomyces fradiae . Journal of Antibiotics 38:1226–1236
    [Google Scholar]
  4. Dyson P., Schrempf H. 1987; Genetic instability and DNA amplification in Streptomyces lividans66. Journal of Bacteriology 169:4796–4803
    [Google Scholar]
  5. Fishman S. E., Hershberger C. L. 1983; Amplified DNA in Streptomyces fradiae . Journal of Bacteriology 155:459–466
    [Google Scholar]
  6. Fishman S. E., Rosteck P. R., Hershberger C. L. 1985; A 2-2-kilobase repeated DNA segment is associated with DNA amplification in Streptomyces fradiae . Journal of Bacteriology 161:199–206
    [Google Scholar]
  7. Flett F., Cullum J. 1987; DNA deletions in spontaneous chloramphenicol-sensitive mutants of Streptomyces coelicolor A3(2) and Streptomyces lividans 66. Molecular and General Genetics 207:499–502
    [Google Scholar]
  8. Freeman R. F., Hopwood D. A. 1978; Unstable naturally occurring resistance to antibiotics in Streptomyces . Journal of General Microbiology 106:377–381
    [Google Scholar]
  9. Freeman R. F., Bibb M. J., Hopwood D. A. 1977; Chloramphenicol acetyltransferase-independent chloramphenicol resistance in Streptomyces coelicolorA3(2). Journal of General Microbiology 98:453–465
    [Google Scholar]
  10. Garvey E. P., Santi D. V. 1986; Stable amplified DNA in drug-resistant Leishmania exists as extrachromosomal circles. Science 233:535–540
    [Google Scholar]
  11. Genthner F. J., Hook L. A., Strohl W. R. 1985; Determination of the molecular mass of bacterial genomic DNA and plasmid copy number by high- pressure liquid chromatography. Applied and Environmental Microbiology 50:1007–1013
    [Google Scholar]
  12. Hara O., Horinouchi S., Uozumi T., Beppu T. 1983; Genetic analysis of A-factor synthesis in Streptomyces coelicolorA3(2) and Streptomyces griseus . Journal of General Microbiology 129:2939–2944
    [Google Scholar]
  13. Hasegawa M., Hintermann G., Simonet J. M., Crameri R., Piret J., Hütter R. 1985; Certain chromosomal regions in Streptomyces glaucescens tend to carry amplifications and deletions. Molecular and General Genetics 200:375–384
    [Google Scholar]
  14. Hintermann G., Crameri R., Vogtli M., Hütter R. 1984; Streptomycin-sensitivity in Streptomyces glaucescens is due to deletions comprising the structural gene coding for a specific phosphotransferase. Molecular and General Genetics 196:513–520
    [Google Scholar]
  15. Hintermann G., Zatchej M., Hütter R. 1985; Cloning and expression of the genetically unstable tyrosinase structural gene from Streptomyces glaucescens . Molecular and General Genetics 200:422–432
    [Google Scholar]
  16. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1982 Genetic Manipulation of Streptomyces. A laboratory Manual Norwich:: The John Innes Foundation;
    [Google Scholar]
  17. Hornemann H., Otto C. J., Hoffman G. G., Bertinuson A. C. 1987; Spectinomycin resistance and associated DNA amplification in Streptomyces achromogenes subsp. rubradiris . Journal of Bacteriology 169:2360–2366
    [Google Scholar]
  18. Hütter R., Kieser T., Crameri R., Hintermann G. 1981; Chromosomal instability in Streptomyces glaucescens . Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (supplement) 11:551–559
    [Google Scholar]
  19. Kinashi H., Shimaji M., Sakai A. 1987; Giant linear plasmids in Streptomyces which code for antibiotic biosynthesis genes. Nature; London: 328454–456
    [Google Scholar]
  20. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Ono H., Hintermann G., Crameri R., Wallis G., Hütter R. 1982; Reiterated DNA sequence in a mutant strain of Streptomyces glaucescens and cloning of the sequence in Escherichia coli . Molecular and General Genetics 186:106–110
    [Google Scholar]
  22. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  23. Robinson M., Lewis E., Napier E. 1981; Occurrence of reiterated DNA sequences in strains of Streptomyces produced by an interspecific protoplast fusion. Molecular and General Genetics 182:336–340
    [Google Scholar]
  24. Schrempf H. 1982; Plasmid loss and changes within the chromosomal DNA of Streptomyces reticuli . Journal of Bacteriology 151:701–707
    [Google Scholar]
  25. Schrempf H. 1983; Deletion and amplification of DNA sequences in melanin-negative variants of Streptomyces reticuli . Molecular and General Genetics 189:501–505
    [Google Scholar]
  26. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  27. Suter M., Hütter R., Leisinger T. 1978; Mutants of Streptomyces glaucescens affected in the production of extracellular enzymes. In Genetics of Actinomycetales pp. 61–64 Freerksen E., Tarnok I., Thumin J. H. Edited by Stuttgart & New York: Gustav Fischer;
    [Google Scholar]
  28. Usdin K., Christians K. M., De Wet C. A., Potgieter T. D., Shaw C. B., Kirby R. 1985; The loss of a large DNA fragment is associated with an aerial mycelium negative (Amy) phenotype of Streptomyces cattleya . Journal of General Microbiology 131:979–981
    [Google Scholar]
  29. Young M., Cullum J. 1987; A plausible mechanism for large scale chromosomal DNA amplification in Streptomyces . FEBS Letters 212:101
    [Google Scholar]
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