1887

Abstract

Several strains of agar-degrading bacteria capable of fixing N were isolated from seawater and eelgrass-bed sediment in Aburatsubo Inlet, Kanagawa, Japan, during the summer of 1986. All strains were Gram-negative, facultatively anaerobic, and required NaCl for growth. They were straight or slightly curved rods and were motile in liquid medium by means of a single polar flagellum. These characteristics as well as the G+C contents of their DNA (44·7–46·1 mol%) placed them in the family Vibrionaceae. These strains produced extracellular agarase on agar medium, yielding reducing sugars and acids as the end products. They expressed significant nitrogenase (acetylene reduction) activities after a few hours of incubation under anaerobic conditions. They utilized combined nitrogen sources both aerobically and anaerobically, but fixed N only under anaerobic conditions. Neither yeast extract nor vitamins were required for N fixation. These strains were demonstrated to fix N anaerobically using agar as the sole carbon source.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-7-1821
1988-07-01
2021-05-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/7/mic-134-7-1821.html?itemId=/content/journal/micro/10.1099/00221287-134-7-1821&mimeType=html&fmt=ahah

References

  1. Agbo J. A. C., Moss M. O. 1979; The isolation and characterization of agarolytic bacteria from a lowland river. Journal of General Microbiology 115:355–368
    [Google Scholar]
  2. Vonborel E., Hostettler F., Deuel H. 1952; Fasciculus 1-15 Quantitative Zuckerbestimmung mit 3,5-Dinitrosalicylsaure und Phenol. Helvetica chimica acta 35:115–120
    [Google Scholar]
  3. Breed R. S., Murray E. G. D., Smith N. R. 1957 Bergey’s Manual of Determinative Bacteriology, 7th edn.. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Donovan T. J., Furniss A. L. 1984; Vibrios, Aeromonas, Plesiomonas, and Photobacterium. In Microbiological Methods, 5th edn. pp. 271–279 Collins C. H., Lyne P. M. Edited by London: Butterworth;
    [Google Scholar]
  5. Guerinot M. L., Colwell R. R. 1985; Enumeration, isolation, and characterization of N2-fixing bacteria from seawater. Applied and Environmental Microbiology 50:350–355
    [Google Scholar]
  6. Guerinot M. L., West P. A., Lee J. V., Colwell R. R. 1982; Vibrio diazotrophicus sp. nov., a marine nitrogen-fixing bacterium. International Journal of Systematic Bacteriology 32:350–357
    [Google Scholar]
  7. HOFSTEN V. B., Malmqvist M. 1975; Degradation of agar by a Gram-negative bacterium. Journal of General Microbiology 87:150–158
    [Google Scholar]
  8. Krieg N. R. 1984 (editor) Bergey’s Manual of Systematic Bacteriology 1 Baltimore: Williams & Wilkins;
    [Google Scholar]
  9. Lindberg I., Uranhall U. 1984; isolation ana characterization of dinitrogen-fixing bacteria from the rhizosphere of temperate cereals and forage grasses. Applied and Environmental Microbiology 48:683–689
    [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  11. Van der meulen H. J., Harder W., Veldkamp H. 1974; Isolation and characterization of Cytophaga flevensis sp. nov., a new agarolytic flexibacterium. Antonie van Leeuwenhoek 40:329–346
    [Google Scholar]
  12. Shieh W. Y., Simidu U., Maruyama Y. 1987; Isolation of a nitrogen-fixing Vibrio species from the roots of eelgrass (Zostera marina). Journal of General and Applied Microbiology 33:321–330
    [Google Scholar]
  13. Watanabe I., Barraquio W. L. 1979; Low levels of fixed nitrogen required for isolation of free-living N2-fixing organisms from rice roots. Nature; London: 111565–566
    [Google Scholar]
  14. Waterbury J. B., Calloway C. B., Turner R. D. 1983; A cellulolytic nitrogen-fixing bacterium cultured from the gland of Deshayes in shipworms (Bivalvia: Teredinidae). Science 221:1401–1403
    [Google Scholar]
  15. West P. A., Brayton P. R., Twilley R. R., Bryant T. N., Colwell R. R. 1985; Numerical taxonomy of nitrogen-fixing 'decarboxylasenegative' Vibrio species isolated from aquatic environments. International Journal of Systematic Bacteriology 35:198–205
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-7-1821
Loading
/content/journal/micro/10.1099/00221287-134-7-1821
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error