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Bacteroids, prepared from stem nodules of Sesbania rostrata inoculated with Rhizobium sp. strain ORS571, in steady-state reactions in which O2 was supplied in solution with soybean leghaemoglobin or mammalian myoglobin as O2 carriers, and with succinate as exogenous substrate, fixed N2 to NH3 with highest rates at 10 nM free O2, where O2 uptake was approximately half-maximal. Higher concentrations (> 100 nM) of free O2, shown previously to be required for optimum nitrogenase activity in O2-trained ORS571 grown in continuous culture, inhibited N2 fixation by stem nodule bacteroids.
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