1887

Abstract

A bacteriophage, designated ϕC69, isolated from a culture of was characterized. The phage propagates on NRRL 2338 but does not infect 10 or 3 species tested. It infects NRRL 2359 but does not produce infectious phage particles in this host. ϕC69 is approximately 40 kb in length and contains cohesive ends. A fragment containing ligated phage DNA ends was cloned in Restriction maps of the phage DNA and the fragment for several enzymes are shown. Transfection of both and with ϕC69 resulted in approximately equal titres of infectious phage particles produced from approximately the same number of regenerating cells. Transfection of with DNA from phages SH10 and KC404 also resulted in the production of infectious phage particles. The basis for differences among hosts in susceptibility to infection by various actinophages is discussed.

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1988-07-01
2024-12-03
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References

  1. Anne J., Verheyen P., Volckaert G., Eyssen H. 1985; A restriction endonuclease map of Streptomyces phage VWB. Molecular and General Genetics 200:506–507
    [Google Scholar]
  2. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacteriocins. Bacteriological Reviews 31:230–314
    [Google Scholar]
  3. Brzezinski R., Surmacz E., Kutner M., Piekarowicz A. 1986; Restriction mapping and close relationship of the DNA of Streptomyces erythraeus phages 121 and SE-5. Journal of General Microbiology 132:2937–2943
    [Google Scholar]
  4. Chater K. F., Bruton C. J. 1983; Mutational cloning in Streptomyces and the isolation of antibiotic production genes. Gene 26:67–78
    [Google Scholar]
  5. Chater K. F., Bruton C. J., Springer W., Suarez J. E. 1981; Dispensible sequences and packaging constraints of DNA from the Streptomyces temperate phage ϕC31. Gene 15:249–256
    [Google Scholar]
  6. Doermann A. H. 1953; The vegetative state in the life cycle of bacteriophage: evidence for its occurrence, and its genetic characterization. Cold Spring Harbor Symposia on Quantitative Biology 18:3–11
    [Google Scholar]
  7. Donadio S., Paladino R., Costanzi I., Sparapani P., Schreil W., Iaccarino M. 1986; Characterization of bacteriophages infecting Streptomyces erythraeus and properties of phage-resistant mutants. Journal of Bacteriology 166:1055–1060
    [Google Scholar]
  8. Dowding J. E. 1973; Characterization of a bacteriophage virulent for Streptomyces coelicolor A3(2). Journal of General Microbiology 76:163–176
    [Google Scholar]
  9. Foor F., Roberts G. P., Morin N., Snyder L., Hwang M., Gibbons P. H., Paradisio M. J., Stotish R. L., Ruby C. L., Wolanski B., Streicher S. L. 1985; Isolation and characterization of the Streptomyces cattelya temperate phage TG1. Gene 39:11–16
    [Google Scholar]
  10. Grund A. D., Hutchinson C. R. 1987; Bacteriophages of Saccharopolyspora erythraea. Journal of Bacteriology 169:3013–3122
    [Google Scholar]
  11. Harris J. E., Chater K. F., Bruton C. J., Piret J. M. 1983; The restriction map of c gene deletions in Streptomyces bacteriophage ϕC31 and their use in cloning vector development. Gene 22:167–174
    [Google Scholar]
  12. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985 Genetic Manipulation of Streptomyces. A Laboratory Manual Norwich:: John Innes Foundation;
    [Google Scholar]
  13. Klaus S., Krugel H., Suss F., Neigenfind M., Zimmermann I., Taubeneck U. 1981; Properties of temperate actinophage SH10. Journal of General Microbioloby 123:269–279
    [Google Scholar]
  14. Kuznetsov V. D., Rodionova E. G., Yangulova I. V., Dmitrieva S. V. 1977; Review of the systematic position of Actinomyces erythraeus Waksman and Curtis 1916 and its transfer to the genus Proactinomyces. Mikrobiologiya 46:137–142
    [Google Scholar]
  15. Labeda D. P. 1987; Transfer of the type strain of Streptomyces erythraeus (Waksman 1923) Waksman and Henrici 1948 to the genus SaccharopolysporaLacey and Goodfellow as Saccharopolyspora erythraea sp. nov., and designation of a neotype strain for Streptomyces erythraeus. International Journal of Systematic Bacteriology 37:19–22
    [Google Scholar]
  16. Morino T., Isogai T., Takahashi H., Saito H. 1983; Cleavage analysis of actinophage R4 and its deletion mutants. Agricultural and Biological Chemistry 47:1873–1878
    [Google Scholar]
  17. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  18. Seno E. T., Bruton C. J., Chater K. F. 1983; The glycerol utilization operon of Streptomyces coelicolor: genetic mapping of gyl mutations and analysis of cloned gyl DNA. Molecular and General Genetics 193:119–128
    [Google Scholar]
  19. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  20. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with universal primers. Gene 19:259–268
    [Google Scholar]
  21. Yamamoto H., Maurer K. H., Hutchinson C. R. 1986; Transformation of Streptomyces erythraeus. Journal of Antibiotics 39:1304–1313
    [Google Scholar]
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