1887

Abstract

is a filamentous, nonheterocystous cyanobacterium which fixes gaseous nitrogen in axenic culture under aerobic conditions. Growth on agar plates reflects the benthic habit observed in the natural environment, and in liquid culture clumps or aggregates are produced. Scanning electron microscopy suggests that these aggregates are primarily the result of random movement of trichomes against one another. The aim of this study was to ascertain whether aggregation or any intracellular differentiation might be important in protection of the oxygen-labile nitrogenase in this species. Observations using light and transmission electron microscopy revealed no cellular or intracellular differentiation, and tetrazolium labelling experiments provided no evidence that a proportion of cells might act in a heterocyst-like fashion. The results presented suggest that nitrogen fixation in is unlikely to be enhanced in oxygen-deficient microsites formed as a result of aggregation.

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1988-07-01
2022-01-23
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References

  1. Allen M. M. 1968; Simple conditions for growth of unicellular blue-green algae on plates. Journal of Phycology 4:1–4
    [Google Scholar]
  2. Baker D., Lending C., Dean D. 1984; Localisation of nitrogenase using monoclonal antibodies. In Advances in Nitrogen Fixation Research p. 249 Veeger C., Newton W. E. Edited by The Hague: Martinus Nijhoff/Dr W. Junk;
    [Google Scholar]
  3. Bothe H., Neuer G., Kalbe I., Eisbrenner G. 1980; Electron donors and hydrogenase in nitrogen-fixing microorganisms. In Nitrogen Fixation pp 83–112 Stewart W. D. P., Gallon J. R. Edited by London: Academic Press;
    [Google Scholar]
  4. Bryceson I., Fay P. 1981; Nitrogen fixation in Oscillatoria (Trichodesmium) erythraea in relation to bundle formation and trichoma differentiation. Marine Biology 61:159–166
    [Google Scholar]
  5. Carpenter E. J., Price C. C. 1976; Marine Oscillatoria (Trichodesmium): explanation for aerobic nitrogen fixation without heterocysts. Science 191:1278–1280
    [Google Scholar]
  6. Fay P., Kulasooriya S. A. 1972; Tetrazolium reduction and nitrogenase activity in heterocystous blue-green algae. Archives of Microbiology 87:341–352
    [Google Scholar]
  7. Gallon J. R. 1980; Nitrogen fixation by photoautotrophs. In Nitrogen Fixation pp 197–238 Stewart W. D. P., Gallon J. R. Edited by London: Academic Press;
    [Google Scholar]
  8. Golterman H. L., Clymo R. S., Ohnstad M. A. M. 1978 Methods for Physical and Chemical Analysis of Fresh Waters, 2nd edn.. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  9. Henry L. E. A., Gogotov I. N., Hall D. O. 1978; Superoxide dismutase and catalase in the protection of the proton-donating system of nitrogen fixation in the blue-green alga Anabaena cylindrica. Biochemical Journal 174:373–377
    [Google Scholar]
  10. Howsley R. 1980 Aspects of the physiology and ecology of cyanobacteria in relation to hydrogen sulphide PhD thesis University of Liverpool;
    [Google Scholar]
  11. Kallas T., Rippka R., Coursin T., Rebiere M.-C., Tandeau De Marsac N., Cohen-Bazire G. 1983; Aerobic nitrogen fixation by nonheterocystous cyanobacteria. In Photosynthetic Prokaryotes: Cell Differentiation and Function pp 281–314 Papageorgiou G. C., Packer L. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  12. Karnovsky M. J. 1965; A formaldehyde-glutaralde-hyde fixative of high osmolality for use in electron microscopy. Journal of Cell Biology 27:137A
    [Google Scholar]
  13. Lamont H. C. 1969; Sacrificial cell death and trichome breakage in an oscillatoriacean blue-green alga: the role of murein. Archives of Microbiology 69:237–259
    [Google Scholar]
  14. Malin G., Walsby A. E. 1985; Chemotaxis of a cyanobacterium on concentration gradients of carbon dioxide, bicarbonate and oxygen. Journal of General Microbiology 131:2643–2652
    [Google Scholar]
  15. Marker A. F. H., Nusch E. A., Rai H., Reimann B. 1980; The measurement of photosynthetic pigments in freshwaters and standardisation of methods: conclusions and recommendations. Archiv fur Hydrobiologie Beihefte - Ergebnisse der Limnologie 14:91–106
    [Google Scholar]
  16. Murray M. A., Benemann J. R. 1980; Localisation of nitrogenase in a heterocystous blue-green alga by immunoferritin labelling. Journal of Phycology (Supplement) 16: Abstract 109
    [Google Scholar]
  17. Paerl H. W. 1985; Microzone formation: its role in the enhancement of aquatic N2 fixation. Limnology and Oceanography 30:1246–1252
    [Google Scholar]
  18. Pearson H. W., Howsley R. 1980; Concomitant photoautotrophic growth and nitrogenase activity by the cyanobacterium Plectonema boryanum in continuous culture. Nature; London: 288263–265
    [Google Scholar]
  19. Pearson H. W., Howsley R., Kjeldsen C. K., Walsby A. E. 1979; Aerobic nitrogenase activity associated with a non-heterocystous filamentous cyanobacterium. FEMS Microbiology letters 5:163–167
    [Google Scholar]
  20. Pearson H. W., Malin G., Howsley R. 1981; Physiological studies on in vivo nitrogenase activity by axenic cultures of the blue-green alga Microcoleus chthonoplastes. (Abstract). British Phycological Journal 16:139
    [Google Scholar]
  21. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  22. Rippka R., Deruelles J., Waterbury J. B., Herdman M., Stanier R. Y. 1979; Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111:1–61
    [Google Scholar]
  23. Shukovsky E. S., Halfen L. N. 1976; Cellular differentiation of terminal regions of Oscillatoria princeps (Cyanophyceae). Journal of Phycology 12:336–342
    [Google Scholar]
  24. Spurr A. R. 1969; A low viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research 26:31–43
    [Google Scholar]
  25. Stal L. J., Krumbein W. E. 1981; Aerobic nitrogen fixation in pure cultures of a benthic Oscillatoria (cyanobacteria). FEMS Microbiology Letters 11:295–298
    [Google Scholar]
  26. Stewart W. D. P. 1980; Some aspects of structure and functions in N2-fixing cyanobacteria. Annual Review of Microbiology 34:497–536
    [Google Scholar]
  27. Stewart W. D. P., Mague T., Fitzgerald G. P., Burris R. H. 1971; Nitrogenase activity in Wisconsin lakes of differing degrees of eutrophication. New Phytologist 70:497–509
    [Google Scholar]
  28. Tel-Or E., Stewart W. D. P. 1977; Photosynthetic components and activities of nitrogen fixing isolated heterocysts. Proceedings of the Royal Society B198:61–86
    [Google Scholar]
  29. Walsby A. E. 1985; The permeability of heterocysts to the gases nitrogen and oxygen. Proceedings of the Royal Society B226:345–366
    [Google Scholar]
  30. Waterbury J. B., Stanier R. Y. 1978; Patterns of growth and development in pleurocapsalean cyanobacteria. Microbiological Reviews 42:2–44
    [Google Scholar]
  31. Winkenbach F., Wolk C. P. 1973; Activities of enzymes of the oxidative and reductive pentose phosphate pathways in heterocysts of a blue-green alga. Plant Physiology 52:480–483
    [Google Scholar]
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