%0 Journal Article %A Okahashi, Nobuo %A Koga, Toshihiko %A Nishihara, Tatsuji %A Fujiwara, Taku %A Hamada, Shigeyuki %T Immunobiological Properties of Lipopolysaccharides Isolated from Fusobacterium nucleatum and F. necrophorum %D 1988 %J Microbiology, %V 134 %N 6 %P 1707-1715 %@ 1465-2080 %R https://doi.org/10.1099/00221287-134-6-1707 %I Microbiology Society, %X Lipopolysaccharides (LPSs) were isolated from Fusobacterium nucleatum ATCC 10953 and F. necrophorum ATCC 25286 by the hot phenol/water procedure. F. nucleatum LPS was composed of 16% (w/w) carbohydrate, 10% (w/w) hexosamine and 40% (w/w) fatty acid, while F. necrophorum LPS was composed of 26% (w/w) carbohydrate, 12% (w/w) hexosamine and 28% (w/w) fatty acid. These LPS preparations induced mitogenic responses in spleen cells of BALB/c, BALB/c (nu/nu) and C3H/HeN mice, and these responses were suppressed by the addition of polymyxin B. The preparations also induced the polyclonal responses of C3H/HeN spleen cells. In addition, enhanced glucose utilization and interleukin-1 production by murine peritoneal macrophages were demonstrated. Neither spleen cells nor macrophages from the ‘LPS-nonresponsive’ C3H/HeJ mouse were activated by LPSs from the Fusobacterium species. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-134-6-1707