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Volume 134, Issue 6

Molecular Cloning of a Gene Affecting the Autolysin Level and Flagellation in Free

Abstract

A 2·8 kb I fragment of 168W DNA has been cloned into HB101 and AG5 using pAC3 as a shuttle plasmid. The new plasmid (pBRG1), of 10·2 kb, complemented mutations which show reduced production of autolysin(s), filamentation and non-motility (deficiency of flagella). Deletion experiments showed that the suppressive gene is located between the III and I sites (1·0 kb apart) in pBRG1. The integration of a plasmid having chloramphenicol resistance closely linked to the gene into the AC703 chromosome and its genetic analysis indicated that the cloned fragment contained the gene itself. A high-copy-number plasmid carrying the cloned gene did not lead to an increase in autolysin production above the wild-type level, but it changed the colony morphology from smooth to rough. Among several autolysin-deficient mutations, was suppressed only by the high-copy-number plasmid carrying the cloned gene.

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© Society for General Microbiology 1988
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1988-06-01
2024-04-23
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Molecular Cloning of a Gene Affecting the Autolysin Level and Flagellation in Bacillus subtilis
Microbiology 134, 1611 (1988); https://doi.org/10.1099/00221287-134-6-1611
/content/journal/micro/10.1099/00221287-134-6-1611
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