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1887

Abstract

Addition of choline to growing led to abnormal cell septation, lack of cell separation and the consequent formation of chains. Similar results were obtained with the wild-type strain N1-4 and its autolysin-deficient mutant N1-4081. With strain N1-4, addition of choline at 1 to 2 mg ml resulted in inhibition of autolysis assessed as autoplast formation in 0·6 -sucrose, lysis by 0·3 -NaCl/0·03 -sodium citrate, lysis by 0·1% Triton X-100 and lysis by penicillin G. data confirmed the inhibition by choline of wall-degrading activity, using N1-4 cell walls as substrate. Choline was shown to be a component of the teichoic acid of N1-4.

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/content/journal/micro/10.1099/00221287-134-6-1603
1988-06-01
2025-01-15
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References

  1. Archibald A. R. 1971; Teichoic acids. Methods in carbohydrate chemistry 6:162–172
    [Google Scholar]
  2. Augustinsson K. B., Grahn M. 1953; The separation of choline esters by paper chromatography. Acta chemica scandinavica 7:906–912
    [Google Scholar]
  3. Briese T., Hakenbeck R. 1984; Influence of lipoteichoic acid and choline on the autolytic enzyme activity of Streptococcus pneumoniae. In Microbial Cell Wall Synthesis and Autolysis pp. 201–206 Nombela C. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  4. Briese T., Hakenbeck R. 1985; Interaction of the pneumococcal amidase with lipoteichoic acid and choline. European Journal of Biochemistry 146:417–427
    [Google Scholar]
  5. Brundish D. E., Baddiley J. 1968; Pneumococcal C-substance, a ribitol teichoic acid containing choline phosphate. Biochemical Journal 110:573–582
    [Google Scholar]
  6. Chargaff E., Levine C., Green C. 1948; Techniques for the demonstration by chromatography of nitrogenous lipide constituents, sulfur-containing amino acids, and reducing sugars. Journal of Biological Chemistry 175:67–71
    [Google Scholar]
  7. Cornett J. B., Shockman G. D. 1978; Cellular lysis of Streptococcus faecalis induced with Triton X-100. Journal of Bacteriology 135:153–160
    [Google Scholar]
  8. De Castro-Costa M. R., Landman O. E. 1977; Inhibitory protein controls the reversion of protoplasts and L forms of Bacillus subtilis to the walled state. Journal of Bacteriology 129:678–689
    [Google Scholar]
  9. Guidicelli S., Tomasz A. 1984; Attachment of pneumococcal autolysin to wall teichoic acids, an essential step in enzymatic wall degradation. Journal of Bacteriology 158:1188–1190
    [Google Scholar]
  10. Höltje J. V., Tomasz A. 1974; Specific recognition of choline residues in the cell wall teichoic acid by the N-acetylmuramyl-l-alanine amidase of Pneumococcus. Journal of Biological Chemistry 250:6072–6076
    [Google Scholar]
  11. Hongo M. 1960; US patent no.2945786.
    [Google Scholar]
  12. Howard L. V., Gooder H. 1974; Specificity of the autolysin of Streptococcus (Diplococcus) pneumoniae. Journal of Bacteriology 117:796–804
    [Google Scholar]
  13. Mosser J. L., Tomasz A. 1970; Choline-contain ing teichoic acid as a structural component of pneumococcal cell wall and its role in sensitivity to lysis by an autolytic enzyme. Journal of Biological Chemistry 245:287–298
    [Google Scholar]
  14. Ogata S., Hongo M. 1973; Bacterial lysis of Clostridium species. I. Lysis of Clostridium species by univalent cation. Journal of General and Applied Microbiology 19:251–261
    [Google Scholar]
  15. Ogata S., Umeda A., Hongo M. 1974; Bacterial lysis of Clostridium species. Effect of fradiomycin on the activities of various lytic enzymes active toward Clostridium saccharoperbutylacetonicum. Journal of the Faculty of Agriculture, Kyushu University 19:49–59
    [Google Scholar]
  16. Ogata S., Choi K. H., Hongo M. 1975; Sucrose-induced autolysis and development of protoplastlike cells of Clostridium saccharoperbutylacetobutylicum. Agricultural and Biological Chemistry 39:1247–1254
    [Google Scholar]
  17. Ogata S., Choi K. H., Hongo M., Hayashida S. 1980; Studies on sucrose-induced autolysis of clostridial cells. 1. Induction of a rapid bacterial autolysis by sucrose treatment. Journal of the Faculty of Agriculture, Kyushu University 25:33–45
    [Google Scholar]
  18. Reysset G., Hubert J., Podvin L., Sebald M. 1987; Protoplast formation and regeneration of Clostridium acetobutylicum strain N1-4080. Journal of General Microbiology 133:2595–2600
    [Google Scholar]
  19. Rogers H. J., Perkins H. R., Ward J. B. 1980 Microbial Cell Walls and Membranes pp 437–460 London: Chapman & Hall;
    [Google Scholar]
  20. Ronda C., Garcia J. L., Garcia E., Sanchez-Puelles J. M., Lopez R. 1987; Biological role of the pneumococcal amidase. Cloning of the lytA gene in Streptococcus pneumoniae. European Journal of Biochemistry 164:621–624
    [Google Scholar]
  21. Sanchez-Puelles J. M., Ronda C., Garcia E., Mendez E., Garcia J. L., Lopez R. 1986; A new peptidoglycan hydrolase in Streptococcus pneumoniae. FEMS Microbiology Letters 35:163–166
    [Google Scholar]
  22. Yoshino S., Ogata S., Hayashida S. 1982; Some properties of autolysin of Clostridium saccharoperbutylacetonicum. Agricultural and Biological Chemistry 46:1243–1248
    [Google Scholar]
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