1887

Abstract

The NAD-dependent glutamate dehydrogenase from spores was purified more than 300-fold. Estimation of by gel filtration gave a value of 98000 whereas after SDS-PAGE one major band of 54000 was found, suggesting that the enzyme is a dimer. The enzyme was virtually dependent on the presence of AMP for activity and showed half-maximal activation at 9·5 and 43 μ-AMP in the direction of amination and deamination respectively. ADP was nearly as effective at 20-fold higher concentrations. Other nucleotide monophosphates were ineffective and nucleoside triphosphates were slightly inhibitory. Hyperbolic kinetics were found for all substrates yielding values of about 10 m for ammonium, 1 m for 2-oxoglutarate and 0·1 m for NADH in the direction of amination, and 10 m for glutamate and 0·7 m for NAD in the direction of deamination.

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1988-06-01
2021-08-01
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