1887

Abstract

Benomyl treatment (at 100 μg ml) of 1001, and other strains derived from it, determined the appearance of morphological mutants similar to those derived from UV irradiation treatment. A permanent alteration in the morphogenesis of these mutant strains determined their inability to grow by budding, to form oval yeast cells or blastospores (Yphenotype) and their growth as long filamentous forms, mostly with the appearance of pseudomycelium, giving rise to rough colonies (R phenotype). In order to carry out a genetic complementation analysis, we isolated morphological mutants that carried other genetic markers (nutritional, conditional lethal) adequate for crosses by means of protoplast fusion. Wild-type hybrids of regular mononuclear oval yeast cells and smooth colonies were obtained by crossing pairs of complementing mutants, whereas hybrids from crosses of non-complementing mutants still retained their morphological alterations. Our results define two complementation groups, which represent two genes relevant for dimorphism, whose alteration interferes with the correct transition from blastospores to mycelium.

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1988-06-01
2024-03-29
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References

  1. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62:315–323
    [Google Scholar]
  2. Haber J. E., Halvorson H. O. 1975; Methods in sporulation and germination of yeasts. Methods in Cell Biology 11:46–69
    [Google Scholar]
  3. Hubbard M. J., Markie D., Poulter R. T. M. 1986; Isolation and morphological characterization of a mycelial mutant of Candida albicans. Journal of Bacteriology 165:61–65
    [Google Scholar]
  4. Kakar S. N., Magee P. T. 1982; Genetic analysis of Candida albicans, identification of different isoleucine-valine, methionine and arginine alleles by complementation. Journal of Bacteriology 151:1247–1252
    [Google Scholar]
  5. Kakar S. N., Partridge R. M., Magee P. T. 1983; A genetic analysis of Candida albicans: isolation of a wide variety of auxotrophs and demonstration of linkage and complementation. Genetics 104:241–255
    [Google Scholar]
  6. Kurtz M. B., Cortelyou M. W., Miller S. M., Lai M., Kirsch D. R. 1987; Development of autonomously replicating plasmids for Candida albicans. Molecular and Cellular Biology 7:209–217
    [Google Scholar]
  7. Manning M., Mitchell T. G. 1980; Morphogenesis of Candida albicans and cytoplasmic proteins associated with differences in morphology, strain, or temperature. Journal of Bacteriology 144:258–273
    [Google Scholar]
  8. Morris N. R., Oakley C. E. 1979; Evidence that p-fluorophenylalanine has a direct effect on tubulin in Aspergillus nidulans. Journal of General Microbiology 114:449–454
    [Google Scholar]
  9. Nombela C., PoméS R., Gil C. 1987; Protoplasts fusion hybrids from Candida albicans morphological mutants. CRC Critical Reviews in Microbiology 15:79–88
    [Google Scholar]
  10. Odds F. C. 1979 Candida and Candidosis Leicester: Leicester University Press;
    [Google Scholar]
  11. Odds F. C. 1985; Morphogenesis in Candida albicans. CRC Critical Reviews in Microbiology 12:45–93
    [Google Scholar]
  12. Pesti M., Ferenczyl L. 1982; Protoplast fusion hybrids of Candida albicans sterol mutants differing in nystatin resistance. Journal of General Microbilogy 128:123–128
    [Google Scholar]
  13. PoméS R., Gil C., Nombela C. 1985; Genetic analysis of Candida albicans morphological mutants. Journal of General Microbiology 131:2107–2113
    [Google Scholar]
  14. PoméS R., Gil C., Cabetas M. D., Nombela C. 1987; Variability of colonial morphology in benomyl-induced morphological mutants from Candida albicans. FEMS Microbiology Letters 48:255–259
    [Google Scholar]
  15. Poulter R., Jeffery K., Hubbard J., Shepherd M. G., Sullivan P. A. 1981; Parasexual genetic analysis of Candida albicans by spheroplast fusion. Journal of Bacteriology 146:833–840
    [Google Scholar]
  16. Sarachek A., Rhoads D. D., Schwarzhoff R. H. 1981; Hybridization of Candida albicans through fusion of protoplasts. Archives of Microbiology 129:1–8
    [Google Scholar]
  17. Sevilla M. J., Odds F. C. 1986; Development of Candida albicans hyphae in different growth media - variations in growth rates, cell dimensions and timing of morphogenetic events. Journal of General Microbiology 132:3083–3088
    [Google Scholar]
  18. Sherman F., Fink G. R., Hicks J. B. 1979 Methods in Yeast Genetics. Laboratory Manual Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Slutsky B., Buffo J., Soll D. R. 1985; High-frequency switching of colony morphology in Candida albicans. Science 230:666–669
    [Google Scholar]
  20. Slutsky B., Staebell M., Anderson J., Risen L., Pfaller M., Soll D. R. 1987; ‘White-Opaque transition’: a second high-frequency switching system in Candida albicans. Journal of Bacteriology 169:189–197
    [Google Scholar]
  21. Soll D. R. 1986; The regulation of cellular differentiation in the dimorphic yeast Candida albicans. BioEssays 5:5–11
    [Google Scholar]
  22. Whelan W. L., Partridge R. M., Magee P. T. 1980; Heterozygosity and segregation in Candida albicans. Molecular and General Genetics 180:107–113
    [Google Scholar]
  23. Whelan W. L., Soll D. R. 1982; Mitotic recombination in Candida albicans: recessive lethal alleles linked to a gene required for methionine biosynthesis. Molecular and General Genetics 187:477–485
    [Google Scholar]
  24. Whelan W. L., Markie D., Kwon-CHUNG K. J. 1986; Complementation analysis of resistance to 5-fluorocytosine in Candida albicans. Antimicrobial Agents and Chemotherapy 29:726–729
    [Google Scholar]
  25. Wood J. S. 1982; Genetic effects of methyl benzimid- azoie-2-yl-carbamate on Saccharomyces cerevisiae. Molecular and Cellular Biology 2:1064–1079
    [Google Scholar]
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