1887

Abstract

Strains of containing the Nif plasmid pRD1 were used to investigate the possibility that haem proteins are involved in the regulation by O of expression. Strains lacking 5-aminolaevulinate synthase (HemA), and hence normally unable to synthesize haem proteins, showed an identical response to O in the presence or absence of added aminolaevulinate (and hence of haem proteins). It was concluded that the regulatory protein NifL is not a haem protein.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-6-1499
1988-06-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/6/mic-134-6-1499.html?itemId=/content/journal/micro/10.1099/00221287-134-6-1499&mimeType=html&fmt=ahah

References

  1. Bergersen F. J., Kennedy C., Hill S. 1982; Influence of low oxygen concentration on derepression of nitrogenase in Klebsiella pneumoniae . Journal of General Microbiology 128:909–915
    [Google Scholar]
  2. Buchanan-Wollaston V., Cannon F. C. 1984; Regulation of nif transcription in Klebsiella pneumon-iae . In Advances in Nitrogen Fixation Research p. 732 Veeger C., Newton W. A. Edited by The Hague: Nijhoff-Junk;
    [Google Scholar]
  3. Cannon F. C. 1980; Genetic studies with diazo- trophs. In Methods for Evaluating Biological Nitrogen Fixation pp 367–413 Bergersen F. J. Edited by Chichester: John Wiley;
    [Google Scholar]
  4. Cannon M., Hill S., Kavanagh E., Cannon F. C. 1985; A molecular genetic study of nif expression in Klebsiella pneumoniae at the level of transcription, translation and nitrogenase activity. Molecular and General Genetics 198:198–206
    [Google Scholar]
  5. Dixon R., Cannon F., Kondorosi A. 1976; Construction of a P-plasmid carrying nitrogen fixation genes from Klebsiella pneumoniae. Nature; London: 260:268–271
    [Google Scholar]
  6. Dixon R., Kennedy C., Kondorosi A., Krishnapillai V., Merrick M. 1977; Complementation analysis of Klebsiella pneumoniae mutants defective in nitrogen fixation. Molecular and General Genetics 157:189–198
    [Google Scholar]
  7. Dixon R., Eady R. R., Espin G., Hill S., Iaccarino M., Kahn D., Merrick M. 1980; Analysis of regulation of Klebsiella pneumoniae nitrogen fixation (nif) gene cluster with gene fusions. Nature; London: 286:128–132
    [Google Scholar]
  8. Drummond M. H., Wootton J. C. 1987; Sequence of nifL from Klebsiella pneumoniae: mode of action and relationship to two families of regulatory proteins. Journal of Molecular Micro-biology 1:37–44
    [Google Scholar]
  9. Dunn S. D. 1986; Effects of modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins in Western blots by monoclonal antibodies. Analytical Biochemistry 157:144–153
    [Google Scholar]
  10. Eady R. R., Issack R., Kennedy C., Postgate J. R., Ratcliffe H. 1978; Nitrogenase synthesis in Klebsiella pneumoniae: comparison of ammonium and oxygen regulation. Journal of General Microbiology 104:277–285
    [Google Scholar]
  11. Filser M., Merrick M., Cannon F. 1983; Cloning and characterization of nifLA regulatory mutations from Klebsiella pneumoniae. Molecular and General Genetics 119:485–491
    [Google Scholar]
  12. Fuhrhop J. H., Smith K. M. 1975; Laboratory methods. In Porphyrins and Metalloporphyrins, pp 757–869 Smith K. M. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  13. Haddock B. A. 1973; The reconstitution of oxidase activity in membranes derived from a 5-aminolevu- linic acid-requiring mutant of Escherichia coli. Biochemical Journal 136:877–884
    [Google Scholar]
  14. Haddock B. A., Schairer H. U. 1973; Electron transport chains of Escherichia coli: reconstitution of respiration in a 5-aminolevulinic acid-requiring mutant. European Journal of Biochemistry 35:34–45
    [Google Scholar]
  15. Hill S. 1976; Influence of atmospheric oxygen concentration on aetylene reduction and efficiency of nitrogen fixation in intact Klebsiella pneumoniae. Journal of General Microbiology 93:335–345
    [Google Scholar]
  16. Hill S., Kennedy C., Kavanagh E., Goldberg R. B., Hanau R. 1981; Nitrogen fixation gene (nifL) involved in the oxygen regulation of nitrogenase synthesis in K. pneumoniae. Nature; London: 290:424–426
    [Google Scholar]
  17. Hill S., Turner G. L., Bergersen F. J. 1984; Synthesis and activity of nitrogenase in Klebsiella pneumoniae exposed to low concentrations of oxygen. Journal of General Microbiology 130:1061–1067
    [Google Scholar]
  18. Kennedy C., Drummond M. 1985; The use of cloned nif regulatory elements from Klebsiella pneumoniae to examine nif regulation in Azotobacter vinelandii. Journal of General Microbiology 131:1787–1795
    [Google Scholar]
  19. Kim Y.-M., Ahn K.-J., Beppu T., Uozumi T. 1986; Nucleotide sequence of the nifLA operon of Klebsiella oxytoca NG13 and characterization of the gene products. Molecular and General Genetics 205:253–259
    [Google Scholar]
  20. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227:680–685
    [Google Scholar]
  21. Merrick M., Hill S., Hennecke H., Hahn M., Dixon R. 1982; Repressor properties of the nifL gene product in Klebsiella pneumoniae. Molecular and General Genetics 185:75–81
    [Google Scholar]
  22. Poole R. K., Waring A. J., Chance B. 1979; The reaction of cytochrome o in Escherichia coli with oxygen: low-temperature and spectral studies. Biochemical Journal 184:379–389
    [Google Scholar]
  23. Redinbaugh M. G., Turley R. B. 1986; Adaptation of the bicinchoninic acid protein assay for use with microtitre plates and sucrose gradient fractions. Analytical Biochemistry 153:267–271
    [Google Scholar]
  24. Rennie R. J., Funell A., Smith B. E. 1978; Immunochemistry of nitrogenase as a probe for the enzyme mechanism: Evidence for multiple forms and an MgATP2- binding site on the MoFe protein. FEBS Letters 91:158–161
    [Google Scholar]
  25. Scott R. I., Poole R. K. 1987; Evidence for unequal segregation of cytochromes at cell division in Escherichia coli. FEMS Microbiology Letters 43:1–4
    [Google Scholar]
  26. Smith P. K., Krohn R. I., Hermanson G. T., Mallia A. K., Gartner F. H., Provenzano M. D., Fujimoto E. K., Goeke N. M., Olson B. J., Klenk D. C. 1985; Measurement of protein using bicinchoninic acid. Analytical Biochemistry 150:76–85
    [Google Scholar]
  27. Thomas P. E., Ryan D., Levin W. 1976; An improved staining procedure for the detection of the peroxidase activity of cytochrome P-450 on sodium dodecyl sulfate polyacrylamide gels. Analytical Biochemistry 75:168–176
    [Google Scholar]
  28. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-6-1499
Loading
/content/journal/micro/10.1099/00221287-134-6-1499
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error