1887

Abstract

Several methods of introducing mobilizable cloning vectors by conjugation into the photosynthetic bacterium have been examined. The efficiency of transfer was sufficiently high to enable a bank of genes in to complement non-photosynthetic mutants of , thus providing a generally applicable method of isolating genes. With a mutant incapable of synthesizing reaction centres and light-harvesting LH1 complexes as a recipient, the transfer of genes encoding reaction centre and light-harvesting LH1 polypeptides was examined in some detail. The spectroscopic and electrophoretic properties of this mutant and the newly photosynthetic transconjugant strain were consistent with the efficient transfer and expression of genes.

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1988-06-01
2021-05-13
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