1887

Abstract

is a naturally occurring sterol auxotroph with an absolute nutritional requirement for one of a small group of structurally related phytosterols. We report here a quantitative study demonstrating that a low, otherwise sub-supportive, concentration (≈0·020–0·050 g ml) of an essential phytosterol (stigmasterol) is adequate for growth of this ciliate, provided that a second, relatively non-specific sterol is available at a higher concentration (1·0 g ml) to allow for membrane biosynthesis. This phenomenon, referred to as sterol synergism, has been observed in a broad taxonomic range of organisms, with the conclusion that small amounts of specific sterols are required to perform some previously unknown, vital metabolic or regulatory function. promises to be an excellent model organism for the elucidation of essential sterol function.

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/content/journal/micro/10.1099/00221287-134-6-1441
1988-06-01
2021-07-27
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References

  1. Bloch K. E. 1983; Sterol structure and membrane function. Critical Reviews in Biochemistry 14:47–92
    [Google Scholar]
  2. Conner R. L. 1957; Interaction of stigmasterol and 2,4-dinitrophenol in the growth of Tetrahymena piriformis . Science 126:698
    [Google Scholar]
  3. Conner R. L., Nakatani M. 1958; Stigmasterol antagonism of certain growth inhibitors for Tetrahymena pyriformis . Archives of Biochemistry and Biophysics 74:175–181
    [Google Scholar]
  4. Conner R. L., Van Wagtendonk W. J. 1955; Steroid requirements of Paramecium aurelia . Journal of General Microbiology 12:31–36
    [Google Scholar]
  5. Conner R. L., Van Wagtendonk W. J., Miller C. A. 1953; The isolation from lemon juice of a growth factor of steroid nature required for growth of a strain of Paramecium aurelia . Journal of General Microbiology 9:434–439
    [Google Scholar]
  6. Conner R. L., Landrey J. R., Kaneshiro E. S., Van Wagtendonk W. J. 1971; The metabolism of stigmasterol and cholesterol by Paramecium aurelia . Biochimica et biophysica acta 239:312–319
    [Google Scholar]
  7. Dahl J. S., Dahl C. E., Bloch K. 1980; Sterols in membranes: growth characteristics and membrane properties of Mycoplasma capricolum cultured on cholesterol and lanosterol. Biochemistry 19:1467–1472
    [Google Scholar]
  8. Dahl J. S., Dahl C. E., Bloch K. 1981; Effect of cholesterol on macromolecular synthesis and fatty acid uptake by Mycoplasma capricolum . Journal of Biological Chemistry 256:87–91
    [Google Scholar]
  9. Guyer W., Bloch K. 1983; The sterol requirement of Tetrahymena setosa HZ-1. Journal of Protozoology 30:625–629
    [Google Scholar]
  10. Holz G. G. Jr Erwin J., Wagner B., Rosenbaum N. 1962; The nutrition of Tetrahymena setifera HZ-1: sterol and alcohol requirements. Journal of Protozoology 9:359–363
    [Google Scholar]
  11. Parks L. W. 1978; Metabolism of sterols in yeast. Critical Reviews in Microbiology 6:301–341
    [Google Scholar]
  12. Pinto W. J., Lozano R., Sekula B. C., Nes W. R. 1983; Stereochemically distinct roles for sterol in Saccharomyces cerevisiae . Biochemical and Biophysical Research Communications 112:47–54
    [Google Scholar]
  13. Pitt M. W. 1980 A study of the growth ofParamecium tetraurelia in bacterized culture MS thesis University of Wisconsin-Madison;
    [Google Scholar]
  14. Rampogal M., Bloch K. 1983; Sterol synergism in yeast. Proceedings of the National Academy of Sciences of the United States of America 80:712–715
    [Google Scholar]
  15. Soldo A. T., Van Wagtendonk W. J. 1967; An analysis of the nutritional requirements for fatty acids of Paramecium aurelia . Journal of Protozoology 14:596–600
    [Google Scholar]
  16. Soldo A. T., Van Wagtendonk W. J. 1969; The nutrition of Paramecium aurelia, stock 299. Journal of Protozoology 16:500–506
    [Google Scholar]
  17. Sonneborn T. M. 1975; Species designations and descriptions of the 14 syngens of Paramecium aurelia . Transactions of the American Microbiological Society 94:155–178
    [Google Scholar]
  18. Van Wagtendonk W. J., Wulzen R. 1950; Physiological and chemical aspects of the antistiffness factor essential for guinea pigs. Vitamins and Hormones 8:69–125
    [Google Scholar]
  19. Van Wagtendonk W. J., Conner R. L., Miller C. A., Rao M. R. R. 1953; Growth requirements of Paramecium aurelia var. 4, stock 51.7, sensitives and killers, in axenic medium. Annals of the New York Academy of Sciences 56:929–937
    [Google Scholar]
  20. Whitaker B. D., Nelson D. L. 1987; Growth support and metabolism of phytosterols in Paramecium tetraurelia . Lipids 22:386–396
    [Google Scholar]
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