1887

Abstract

The transport and catabolism of C-dicarboxylic acids have been further studied in MNF3841. Uptake of [C]succinate was induced in free-living cells of strain MNF3841 within 12 min of exposure to succinate and reached a maximum rate within 60 min. Free-living cells of strain MNF3841 oxidizing fumarate accumulated pyruvate when treated with arsenite, an inhibitor of pyruvate dehydrogenase. Generation of pyruvate from C-dicarboxylates was accomplished by malic enzyme. Although malic enzyme was present in free-living cells grown on sucrose, higher activities were observed when fumarate or -arabinose was the growth substrate. In crude extracts, malic enzyme activity required either NAD or NADP as cofactor, together with Mn, and was stimulated by K.

Bacteroids from pea nodules transported [C]succinate immediately after isolation, and contained twofold higher activities of both malic enzyme and pyruvate dehydrogenase than those found in fumarate-grown free-living cells. These data indicate that C-dicarboxylates are available to nodule bacteroids and that they are catabolized through the tricarboxylic acid cycle using malic enzyme and pyruvate dehydrogenase to generate acetyl-CoA.

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1988-06-01
2021-10-21
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References

  1. Arwas R., Mckay I. A., Rowney F. R. P., Dilworth M. J., Glenn A. R. 1985; Properties of organic acid utilization mutants of Rhizobium leguminosarum strain 300. Journal of General Microbiology 131:2059–2066
    [Google Scholar]
  2. Arwas R., Glenn A. R., Mckay I. A., Dilworth M. J. 1986; Properties of double mutants of Rhizobium leguminosarum which are defective in the utilization of dicarboxylic acids and sugars. Journal of General Microbiology 132:2743–2747
    [Google Scholar]
  3. Bernt E., Bergmeyer H. U. 1974; l-Glutamate.UV-assay with glutamate dehydrogenase and NAD. In Methods of Enzymatic Analysis, 2nd edn.. 4 pp. 1704–1708 Bergmeyer H. U. Edited by New York: Academic Press;
    [Google Scholar]
  4. Broughton W. J., Dilworth M. J. 1971; Control of leghaemoglobin synthesis in snake beans. Biochemical Journal 125:1075–1080
    [Google Scholar]
  5. Brown C. M., Dilworth M. J. 1975; Ammonia assimilation by Rhizobium cultures and bacteroids. Journal of General Microbiology 86:39–48
    [Google Scholar]
  6. Bucher T. A., Pfleiderer G. 1955; Pyruvate kinase from muscle. Methods in Enzymology 1:435–440
    [Google Scholar]
  7. Dilworth M. J., Mckay I. A., Franklin M., Glenn A. R. 1983; Catabolite effects on enzyme induction and substrate utilization in Rhizobium leguminosarum. Journal of General Microbiology 129:359–366
    [Google Scholar]
  8. Dilworth M. J., Arwas R., Mckay I. A., Saroso S., Glenn A. R. 1986; Pentose metabolism in Rhizobium leguminosarum MNF300 and in cowpea Rhizobium NGR234. Journal of General Microbiology 132:2733–2742
    [Google Scholar]
  9. Duncan M. J. 1981; Properties of Tn5-induced carbohydrate mutants in Rhizobium meliloti. Journal of General Microbiology 122:61–67
    [Google Scholar]
  10. Finan T. M., Wood J. M., Jordan D. C. 1981; Succinate transport in Rhizobium leguminosarum. Journal of Bacteriology 148:193–202
    [Google Scholar]
  11. Finan T. M., Wood J. M., Jordan D. C. 1983; Symbiotic properties of C4-dicarboxylic acid transport mutants of Rhizobium leguminosarum. Journal of Bacteriology 154:1403–1413
    [Google Scholar]
  12. Glenn A. R., Brewin N. J. 1981; Succinate- resistant mutants of Rhizobium leguminosarum. Journal of General Microbiology 126:237–241
    [Google Scholar]
  13. Glenn A. R., Poole P. S., Hudman J. F. 1980; Succinate uptake by free-living and bacteroid forms of Rhizobium leguminosarum. Journal of General Microbiology 119:267–271
    [Google Scholar]
  14. Glenn A. R., Arwas R., Mckay I. A., Dilworth M. J. 1984a; Fructose metabolism in wild- type, fructokinase-negative and revertant strains of Rhizobium leguminosarum. Journal of General Microbiology 130:231–237
    [Google Scholar]
  15. Glenn A. R., Mckay I. A., Arwas R., Dilworth M. J. 1984b; Sugar metabolism and the symbiotic properties of carbohydrate mutants of Rhizobium leguminosarum. Journal of General Microbiology 130:239–245
    [Google Scholar]
  16. Hansen E. J., Juni E. 1975; Isolation of mutants of Escherichia coli lacking NAD- and NADP-linked malic enzyme activities. Biochemical and Biophysical Research Communications 65:559–566
    [Google Scholar]
  17. Johnston A. W. B., Beringer J. E. 1975; Identification of the Rhizobium strains in pea root nodules using genetic markers. Journal of General Microbiology 87:343–350
    [Google Scholar]
  18. Krebs A., Bridger W. A. 1980; The kinetic properties of phosphoenolpyruvate carboxykinase of Escherichia coli. Canadian Journal of Biochemistry 58:309–318
    [Google Scholar]
  19. Mckay I. A., Glenn A. R., Dilworth M. J. 1985; Gluconeogenesis in Rhizobium leguminosarum MNF3841. Journal of General Microbiology 131:2067–2073
    [Google Scholar]
  20. Plaut G. E., Lardy H. A. 1949; The oxaloacetate decarboxylase of Azotobacter vinelandii. Journal of Biological Chemistry 180:13–27
    [Google Scholar]
  21. Ronson C. W., Primrose S. B. 1979; Carbohydrate metabolism in Rhizobium trifolii: identification and symbiotic properties of mutants. Journal of General Microbiology 112:77–88
    [Google Scholar]
  22. Ronson C. W., Lyttleton P., Robertson J. G. 1981; C4-dicarboxylate transport mutants of Rhizobium trifolii form ineffective nodules on Trifolium repens. Proceedings of the National Academy of Sciences of the United States of America 78:4284–4288
    [Google Scholar]
  23. Sanadi D. R., Langley M., White F. 1959; α-Ketoglutaric acid dehydrogenase. VII. The role of thioctic acid. Journal of Biological Chemistry 234:183–187
    [Google Scholar]
  24. Saroso S., Dilworth M. J., Glenn A. R. 1984; Carbon utilization by free-living and bacteroid forms of cowpea Rhizobium strain NGR234. Journal of General Microbiology 130:1809–1814
    [Google Scholar]
  25. Visser J., Kester H., Jayaseelan K., Topp R. 1982; Pyruvate dehydrogenase complex from Bacillus. Methods in Enzymology 89:399–407
    [Google Scholar]
  26. Von Korf R. W. 1967; Ion-exchange chromatography of citric acid cycle components and related compounds. Methods in Enzymology 13:425–430
    [Google Scholar]
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