1887

Abstract

Sixty strains of belonging to enteropathogenic serogroups (EPEC) were examined for the ability to adhere to HEp-2 cells, the possession of the genes encoding EPEC adherence factor (EAF) and the ability to express an outer-membrane protein (OMP) of 94 kDa thought to be involved in bacterial adhesion to eukaryotic cells. An absolute correlation was found between HEp-2 adhesion and the possession of the genes encoding EAF. An OMP of 94 kDa was observed in the SDS-PAGE profile of most adhesive strains. In some strains this protein was prone to proteolytic degradation. An antiserum raised to a HEp-2 adhesive strain of EPEC did not react with the 94 kDa OMP of all EPEC which were EAF-positive and HEp-2 adhesive, indicating some interstrain antigenic variation of this protein. Although this 94 kDa protein was surface-exposed, specific antibodies binding to the 94 kDa protein in the outer membrane did not interfere with adhesion of EPEC to HEp-2 cells. Therefore, these studies question the value of this protein as a potential vaccine component.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-5-1315
1988-05-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/5/mic-134-5-1315.html?itemId=/content/journal/micro/10.1099/00221287-134-5-1315&mimeType=html&fmt=ahah

References

  1. Baldini M. M., Kaper J. B., Levine M. M., Candy D. C. A., Moon H. W. 1983; Plasmid-mediated adhesion in enteropathogenic Escherichia coli. Journal of Pediatric Gastroenterology and Nutrition 2:534–538
    [Google Scholar]
  2. Chart H., Griffiths E. 1985; Antigenic and molecular homology of the ferric enterobactin receptor protein of Escherichia coli. Journal of General Microbiology 131:1503–1509
    [Google Scholar]
  3. Cravioto A., Gross R. J., Scotland S. M., Rowe B. 1979; An adhesive factor found in strains of Escherichia coli belonging to the traditional infantile enteropathogenic serotypes. Current Microbiology 3:95–99
    [Google Scholar]
  4. Edelman R., Levine M. M. 1983; Summary of a workshop on enteropathogenic Escherichia coli. Journal of Infectious Diseases 147:1108–1118
    [Google Scholar]
  5. Gross R. J., Rowe B. 1985; Escherichia coli diarrhoea. Journal of Hygiene 95:531–550
    [Google Scholar]
  6. Johnstone A., Thorpe R. 1982 Immunochemistry in Practice Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  7. Knutton S., Lloyd D. R., Mcneish A. S. 1987; Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa. Infection and Immunity 55:69–77
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  9. Levine M. M., Nataro J. P., Karch H., Baldini M. M., Kaper J. B., Black R. E., Clements M. L., O’Brien A. D. 1985; The diarrheal response of humans to some classic serotypes of enteropathogenic Escherichia coli is dependent on a plasmid encoding an enteroadhesive factor. Journal of Infectious Diseases 152:550–559
    [Google Scholar]
  10. Van der Ley P., Kuipers O., Tommassen J., Lugtenberg B. 1986; O-antigenic chains of lipopolysaccharide prevent the binding of antibody molecules to an outer membrane pore protein in Enterobacteriaceae. Microbial Pathogenesis 1:43–49
    [Google Scholar]
  11. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Nataro J. P., Baldini M. M., Kaper J. B., Black R. E., Bravo N., Levine M. M. 1985a; Detection of an adherence factor of enteropathogenic Escherichia coli with a DNA probe. Journal of Infectious Diseases 3:560–565
    [Google Scholar]
  13. Nataro J. P., Scaletsky I. C. A., Kaper J. B., Levine M. M., Trabulsi L. R. 1985b; Plasmid- mediated factors conferring diffuse and localized adherence of enteropathogenic Escherichia coli. Infection and Immunity 48:378–383
    [Google Scholar]
  14. Rothbaum R., Mcadams A. J., Giannella R., Partin J. C. 1982; A clinicopathologic study of enterocyte-adherent Escherichia coli: a cause of protracted diarrhea in infants. Gastroenterology 83:441–454
    [Google Scholar]
  15. Scaletsky I. C. A., Silva M. L. M., Trabulsi L. R. 1984; Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infection and Immunity 45:534–536
    [Google Scholar]
  16. Scaletsky I. C. A., Silva M. L. M., Toledo M. R. F., Davis B. R., Blake P. A., Trabulsi L. R. 1985; Correlation between adherence to HeLa cells and serogroups, serotypes, and biotypes of Escherichia coli. Infection and Immunity 49:528–532
    [Google Scholar]
  17. Scotland S. M., Richmond J. E., Rowe B. 1983; Adhesion of enteropathogenic strains of Escherichia coli (EPEC) to HEp-2 cells is not dependent on the presence of fimbriae. FEMS Microbiology Letters 20:191–195
    [Google Scholar]
  18. Stevenson P., Griffiths E. 1985; Detection of antibodies and antigens by immunoblotting. In The Virulence of Escherichia coli (Society for General Microbiology Special Publication13 pp. 457–464 Sussman M. Edited by London: Academic Press;
    [Google Scholar]
  19. Taylor J. 1961; Host-specificity and enteropatho- genicity of Escherichia coli. Journal of Applied Bacteriology 24:316–325
    [Google Scholar]
  20. Ulshen M. H., Rollo J. L. 1980; Pathogenesis of Escherichia coli gastroenteritis in man - another mechanism. New England Journal of Medicine 302:99–101
    [Google Scholar]
  21. Willshaw G. A., Smith H. R., Scotland S. M., Rowe B. 1985; Cloning of genes determining the production of Vero cytotoxin by Escherichia coli. Journal of General Microbiology 131:3047–3053
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-5-1315
Loading
/content/journal/micro/10.1099/00221287-134-5-1315
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error