1887

Abstract

The kinetics of levansucrase secretion were examined in a strain of which overproduces the enzyme ( ). Pulse-labelling experiments indicated that the second step of the levansucrase secretion process has the properties of a membrane active-transport system. This event appears to be directly linked to the influx of iron into the bacteria. The response of to the inducer of levansucrase synthesis was modulated by ferrichelators and the extent of the response varied with the nature of the ferrichelator. Ferridihydroxybenzoate markedly shortened the induction lag period. It is inferred from these data that iron occurs as a cofactor for components of the membrane sites of synthesis/secretion of levansucrase.

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1988-05-01
2024-12-06
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References

  1. Avrameas S., Ternynck T. 1969; The crosslinking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immuno chemistry 6:53–65
    [Google Scholar]
  2. Baskin L., Yang C. S. 1982; Cross-linking studies of the protein topography of rat liver microsomes. Biochimica et biophysica acta 684:263–271
    [Google Scholar]
  3. Benyahia F., Petit-Glatson M. F. 1985; Preferential synthesis, localisation and solubilisation of a precursor form of Bacillus subtilis levansucrase an Escherichia coli minicell system. FEMS microbiolgy Letters 30:155–160
    [Google Scholar]
  4. Berg J. 1986; Potential metal-binding domains in nucleic acid binding proteins. Science 242:485–486
    [Google Scholar]
  5. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  6. Burnette W. N. 1981; ‘Western blotting’: electrophoresis transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  7. Chambert R., Gonzy-Treboul G. 1976; Levan- sucrase of Bacillus subtilis.Characterization of a stabilized fructosyl-enzyme complex and identification of an aspartyl residue as the binding site of the fructosyl group. European Journal of Biochemistry 71:493–508
    [Google Scholar]
  8. Chambert R., Petit-Glatron M. F. 1984; Hyperproduction of exocellular levansucrase by Bacillus subtilis: examination of the phenotype of a sacUb strain. Journal of General Microbiology 130:3143–3152
    [Google Scholar]
  9. Dedonder R. 1966; Levansucrase from Bacillus subtilis. Methods in Enzymology 8:500–506
    [Google Scholar]
  10. Delobbe A. 1968; Le role de fer dans la production, la purification de la levane-sucrase. Effets sur l'enzyme purifie. Bulletin de la Societe de chimie biologique 50:641–673
    [Google Scholar]
  11. Freudl R., Schwartz H., Stierhof Y. D., Gamon K., Hindennach I., Henning U. 1986; An outer membrane protein (OmpA) of Escherichia coli K12 undergoes a conformational change during export. Journal of Biological Chemistry 261:11355–11361
    [Google Scholar]
  12. Gething M. J., Mccammon K., Sambrook J. 1986; Expression of wild-type and mutant forms of influenza hemagglutinin: the role of folding in intracellular transport. Cell 46:939–950
    [Google Scholar]
  13. Grof P., Aslanian D., Chambert R. 1988; Raman spectroscopic study of Bacillus subtilis extracellular levansucrase; effects of ferric ions on the protein conformation. Journal of Raman Spectroscopy (in the press)
    [Google Scholar]
  14. Himeno T., Imanaka T., Aiba S. 1986; Protein secretion in Bacillus subtilis as influenced by the combination of signal sequence and the following mature portion. FEMS Microbiology Letters 35:17–21
    [Google Scholar]
  15. Joliffe L. K., Doyle R. J., Streips U. N. 1981; The energized membrane and cellular autolysis in Bacillus subtilis. Cell 25:753–763
    [Google Scholar]
  16. Kreis T. E., Lodish H. F. 1986; Oligomerization is essential for transport of vesicular stomatitis viral glycoprotein to the cell surface. Cell 46:929–937
    [Google Scholar]
  17. Kunst F., Pascal M., Lepesant-Kejzlarova J., Lepesant J. A., Billault A., Dedonder R. 1974; Pleiotropic mutations affecting sporulation conditions and the syntheses of extracellular enzymes in Bacillus subtilis 168. Biochimie 56:1481–1489
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  19. Lebrun E., Van Rapenbusch R. 1980; The structure of Bacillus subtilis levansucrase at 3·8 A resolution. Journal of Biological Chemistry 255:12034–12036
    [Google Scholar]
  20. Le Coq D., Ratet P., Steinmetz M., Gay P. 1984; A genetic approach to levansucrase secretion in Bacillus subtilis. In Genetics and Biotechnology of Bacilli pp. 141–152 Ganesan A. T., Hoch J. A. Edited by New York: Academic Press;
    [Google Scholar]
  21. Maher P. A., Singer S. J. 1986; Disulfide bonds and the translocation of proteins across membranes. Proceedings of the National Academy of Sciences of the United States of America 83:9001–9005
    [Google Scholar]
  22. Neilands J. B. 1982; Microbial envelope proteins related to iron. Annual Review of Microbiology 36:285–309
    [Google Scholar]
  23. Peters W. J., Warren R. A. J. 1970; The mechanism of iron uptake in Bacillus subtilis. Canadian Journal of Microbiology 16:1285–1291
    [Google Scholar]
  24. Petit-Glatron M. F., Chambert R., Steinmetz M. 1980; Levansucrase of Bacillus subtilis: characterization of a form isolated from phenol treated cells and activated by Triton X-100. European Journal of Biochemistry 103:189–195
    [Google Scholar]
  25. Petit-Glatron M. F., Benyahia F., Chambert R. 1987; Secretion of Bacillus subtilis levansucrase: a possible two-step mechanism. European Journal of Biochemistry 163:379–387
    [Google Scholar]
  26. Randall L., Hardy S. J. S. 1986; Correlation of competence for export with lack of tertiary structure of the mature species: a study in vivo of maltosebinding protein in E. coli. Cell 46:921–928
    [Google Scholar]
  27. Rothman J. E., Kornberg R. D. 1986; An unfolding story of protein translocation. Nature; London: 322209–210
    [Google Scholar]
  28. Smith H., Bron S., Van Ee J., Venema G. 1987; Construction and use of signal sequence selection vectors in Escherichia coli and Bacillus subtilis. Journal of Bacteriology 169:3321–3328
    [Google Scholar]
  29. Tangeras A., Flatmark T., Backstrom D., Ehrenberg A. 1980; Mitochondrial iron not bound in heme and iron sulfur centers, estimation, compartmentation and redox state. Biochimica et biophysic aacta 539:162–175
    [Google Scholar]
  30. Wickner W. T., Lodish H. F. 1985; Multiple mechanisms of protein insertion into and across membranes. Science 230:400–407
    [Google Scholar]
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