1887

Abstract

Auxotrophic mutants of 104A14 were isolated using nitrous acid mutagenesis followed by penicillin enrichment. Mutants in ornithine transcarbamylase, argininosuccinate synthetase or serine-glycine biosynthesis formed nitrogen-fixing (Fix) nodules on the roots of alfalfa (). Mutants with defects in ornithine, pyrimidine, purine, asparagine, leucine, methionine or tyrosine biosynthesis, in one-carbon metabolism or in carbamoylphosphate synthetase formed nodules but these nodules were unable to fix nitrogen. Prototrophic revertants were always Fix. Plasmids that would complement many of these auxotrophs were isolated by transduction with a P2 cosmid gene bank of 104A14. These plasmids were then introduced into mutants of the same and different classes and the growth and symbiotic phenotypes of the new strains were determined. In all cases, complementation of the nutritional defect restored symbiotic nitrogen fixation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-4-913
1988-04-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/4/mic-134-4-913.html?itemId=/content/journal/micro/10.1099/00221287-134-4-913&mimeType=html&fmt=ahah

References

  1. Davis R. W., Botstein D., Roth J. R. 1980 Advanced Bacterial Genetics, a Manual for Genetic Engineering p. 209 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  2. Denarié J., Truchet G., Bergeron B. 1976; Effects of some mutations on symbiotic properties of Rhizobium. In Symbiotic Nitrogen Fixation, pp. 47–61 Nutman P. S. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  3. Fedorov S. N., Zaretskaya A. N. 1977; Ethylmethanesulfonate induction of auxotrophic mutants of Rhizobium meliloti and their characteristics. Mikrobiologiya 47:728–732
    [Google Scholar]
  4. Holmes D. S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
    [Google Scholar]
  5. Kahn M., Kolter R., Thomas C., Figurski D., Meyer R., Remaut E., Helinski D. R. 1979; Plasmid cloning vehicles derived from plasmids ColEl, F, R6K and RK2. Methods in Enzymology 68:268–280
    [Google Scholar]
  6. Kahn M. L., Kraus J., Somerville J. E. 1985; A model of nutrient exchange in the Rhizobium-legume symbiosis. In Nitrogen Fixation Research Progress pp. 193–199 Evans H. J., Bottomley P. J., Newton W. E. Edited by Dordrecht, the Netherlands: Martinus Nijhoff;
    [Google Scholar]
  7. Kerppola T. K., Kahn M. L. 1988; Genetic analysis of carbamoylphosphate synthesis in Rhizobium meliloti 104A14. Journal of General Microbiology 134:921–929
    [Google Scholar]
  8. Malek W., Kowalski M. 1977; Auxotrophic mutations related to symbiotic properties of Rhizobium meliloti strain L5-30. Acta microbiologica polonica 26:345–350
    [Google Scholar]
  9. Mergeay M., Gigot D., Beckmann J., Glansdorff N., Piérard A. 1974; Physiology and genetics of carbamoylphosphate synthesis in Escherichia coli K12. Molecular and General Genetics 133:299–316
    [Google Scholar]
  10. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Ong S. A., Peterson T., Neilands J. B. 1979; Agrobactin, a siderophore from Agrobacterium tumefaciens. Journal of Biological Chemistry 254:1860–1865
    [Google Scholar]
  12. Pain A. N. 1979; Symbiotic properties of antibiotic-resistant and auxotrophic mutants of Rhizobium leguminosarum. Journal of Applied Bacteriology 47:53–64
    [Google Scholar]
  13. Scherrer A., Denarié J. 1971; Symbiotic properties of some auxotrophic mutants of Rhizobium meliloti and of their prototrophic revertants. Plant and Soil (special volume) pp. 39–45
    [Google Scholar]
  14. Somerville J. E., Kahn M. L. 1983; Cloning of the glutamine synthetase I gene from Rhizobium meliloti. Journal of Bacteriology 156:168–176
    [Google Scholar]
  15. Sutton W. D. 1983; Nodule development and senescence. In Nitrogen Fixation, 15 pp. 144–212 Broughton W. Edited by New York: Academic Press;
    [Google Scholar]
  16. Tabor C. W., Tabor H. 1985; Polyamines in microorganisms. Microbiological Reviews 49:81–99
    [Google Scholar]
  17. Truchet G., Michel M., Denarié J. 1980; Sequential analysis of the organogenesis of lucerne (Medicago sativa) root nodules using symbiotically defective mutants of Rhizobium meliloti. Differentiation 16:163–172
    [Google Scholar]
  18. Verma D. P. S., Long S. R. 1983; The molecular biology of the Rhizobium-legume symbiosis. International Review of Cytology (supplement) 14:211–245
    [Google Scholar]
  19. Vincent J. M. 1970 A Manual for the Practical Study of Root Nodule Bacteria Oxford: Blackwell Scientific Publications;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-4-913
Loading
/content/journal/micro/10.1099/00221287-134-4-913
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error