Kinetic and Energetic Aspects of Inorganic Sulphur Compound Oxidation by Free

Abstract

Whole organisms of oxidize thiosulphate to sulphate with the obligatory formation of tetrathionate as an intermediate. Oxidation of thiosulphate to tetrathionate shows an apparent of about 120 and is relatively insensitive to FCCP (carbonyl cyanide -trifluoromethoxyphenylhydrazone), HQNO (2-heptyl-4-hydroxyquinoline--oxide) or thiocyanate. Oxidation of tetrathionate to sulphate shows a of about 27 and is strongly inhibited by FCCP, HQNO, thiocyanate and gramicidin. Sulphite oxidation is also inhibited by FCCP and HQNO. Trithionate oxidation to sulphate occurred and showed unexplained dependence on the presence of sulphate ions. A H/O quotient of about 4 for proton translocation driven by substrate oxidation was seen for each of thiosulphate, tetrathionate and sulphite. ATP synthesis coupled to thiosulphate oxidation was completely abolished by FCCP. The results obtained are consistent with the oxidation of thiosulphate (and probably trithionate) to tetrathionate in the periplasm of the cell, with HQNO-insensitive electron transport to cytochrome , and with further oxidation of tetrathionate (and sulphite) to sulphate after FCCP-sensitive transport to the cytoplasmic side of the membrane. The latter oxidations involve HQNO-sensitive electron transport via cytochrome Inhibition of tetrathionate metabolism by thiocyanate and gramicidin would be consistent with tetrathionate transport by a SO/4H symport process. The proton translocation experiments indicate the mechanism of H extrusion to depend on electron transfer within the quinone/cytochromes segment of the respiratory chain, and does not involve a proton-pumping oxidase. The sulphur-compound-oxidizing system of is shown to be quite different from that previously described for

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-4-865
1988-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/4/mic-134-4-865.html?itemId=/content/journal/micro/10.1099/00221287-134-4-865&mimeType=html&fmt=ahah

References

  1. Harold F. M. 1986 The Vital Force: a Study of Bioenergetics pp 231–246 New York: W. H. Freeman;
    [Google Scholar]
  2. Hatefi Y. 1985; The mitochondrial electron transport and oxidative phosphorylation system. Annual Review of Biochemistry 54:1015–1069
    [Google Scholar]
  3. Hooper A. B., Dispirito A. A. 1985; In bacteria which grow on simple reductants, generation of a proton gradient involves extracytoplasmic oxidation of substrate. Microbiological Reviews 49:140–157
    [Google Scholar]
  4. Kelly D. P. 1978; Bioenergetics of chemolithotrophic bacteria. In Companion to Microbiology, pp 363–386 Bull A. T., Meadow P. M. Edited by London: Longman;
    [Google Scholar]
  5. Kelly D. P. 1982; Biochemistry of the chemolithotrophic oxidation of inorganic sulphur. Philosophical Transactions of the Royal Society B298:499–528
    [Google Scholar]
  6. Kelly D. P. 1985; Physiology of the thiobacilli: elucidating the sulphur oxidation pathway. Microbiological Sciences 2:105–109
    [Google Scholar]
  7. Kelly D. P., Kuenen J. G. 1984; Ecology of the colourless sulphur bacteria. In Aspects of Microbial Metabolism and Ecology, pp. 211–240 Codd G. A. Edited by Orlando: Academic Press;
    [Google Scholar]
  8. Kelly D. P., Syrett P. J. 1966; [35S]Thiosulphate oxidation by Thiobacillus strain C. Biochemical Journal 98:537–545
    [Google Scholar]
  9. Kelly D. P., Chambers L. A., Trudinger P. A. 1969; Cyanolysis and spectrophotometric estimation of trithionate in mixture with thiosulfate and tetrathionate. Analytical Chemistry 41:898–901
    [Google Scholar]
  10. Kelly D. P., Mason J., Wood A. P. 1987; Energy metabolism in chemolithotrophs. In Microbial Growth on C1 Compounds, pp 186–194 Van Verseveld H. W., Duine J. A. Edited by Dordrecht: Martinus Nijhoff;
    [Google Scholar]
  11. Lu W.-P. 1986; A periplasmic location for the thiosulphate-oxidizing multi-enzyme system from Thiobacillus versutus. . FEMS Microbiology Letters 34:313–317
    [Google Scholar]
  12. Lu W.-P., Kelly D. P. 1983a; Thiosulphate oxidation, electron transport and phosphorylation in cell-free systems from Thiobacillus A2. Journal of General Microbiology 129:1661–1671
    [Google Scholar]
  13. Lu W.-P., Kelly D. P. 1983b; Partial purification of a thiosulphate-oxidizing system from Thiobacillus A2. Journal of General Microbiology 129:1673–1681
    [Google Scholar]
  14. Lu W.-P., Swoboda B.E.P., Kelly D. P. 1985; Properties of the thiosulphate-oxidizing multienzyme system from Thiobacillus versutus. . Biochimica et biophysica acta 828:116–122
    [Google Scholar]
  15. Lu W.-P., Kelly D. P. 1988a; Respiration-driven proton translocation in Thiobacillus versutus and the role of the periplasmic thiosulphate-oxidizing enzyme system. Archives of Microbiology 149:297–302
    [Google Scholar]
  16. Lu W.-P., Kelly D. P. 1988b; Cellular location and partial purification of ‘thiosulphate-oxidizing enzyme’ and ‘trithionate hydrolyase’ from Thiobacillus tepidarius. . Journal of General Microbiology 134:877–885
    [Google Scholar]
  17. Mason J., Kelly D. P., Wood A. P. 1987; Chemolithotrophic and autotrophic growth of Ther- mothrix thiopara and some thiobacilli on thiosulphate and polythionates, and a reassessment of the growth yields of T. thiopara in chemostat culture. Journal of General Microbiology 133:1249–1256
    [Google Scholar]
  18. Mitchell P., Moyle J. 1965; Stoichiometry of proton translocation through the respiratory chain and adenosine triphosphatase systems of rat liver mitochondria. Nature, London 208:147
    [Google Scholar]
  19. Okuzumi M. 1966; Reduction of trithionate by Thiobacillus thiooxidans. . Agricultural and Biological Chemistry 30:713–716
    [Google Scholar]
  20. Scholes P., Mitchell P. 1970; Respiration- driven proton translocation in Micrococcus denitrificans. . European Journal of Biochemistry 1:309–323
    [Google Scholar]
  21. Trudinger P. A. 1964; The metabolism of trithionate by Thiobacillus X. Australian Journal of Biological Sciences 17:459–468
    [Google Scholar]
  22. Trudinger P. A. 1965; On the permeability of Thiobacillus neapolitanus to thiosulphate. Australian Journal of Biological Sciences 18:563–568
    [Google Scholar]
  23. Wikström M., Krab K., Saraste M. 1981; Proton-translocating cytochrome complexes. Annual Review of Biochemistry 50:623–655
    [Google Scholar]
  24. Wood A. P., Kelly D. P. 1985; Physiological characteristics of a new thermophilic, obligately chemolithotrophic Thiobacillus species, Thiobacillus tepidarius. . International Journal of Systematic Bacteriology 35:434–437
    [Google Scholar]
  25. Wood A. P., Kelly D. P. 1986; Chemolithotrophic metabolism of the newly isolated moderately thermophilic, obligately autotrophic Thiobacillus tepidarius. . Archives of Microbiology 144:71–77
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-4-865
Loading
/content/journal/micro/10.1099/00221287-134-4-865
Loading

Data & Media loading...

Most cited Most Cited RSS feed