1887

Abstract

We compared four decontamination methods for the isolation of mycobacteria from soil specimens. Different media were used: Lwenstein-Jensen, Ogawa and various modified Ogawa media. Statistical analysis demonstrated that the best results (low contamination and high positivity rates) were obtained when the specimens were incubated in trypticase soy broth, treated with solutions containing malachite green and cycloheximide, then decontaminated with sodium hydroxide and inoculated onto Ogawa media. The lowest contamination rates were obtained with Ogawa medium containing 500 g cycloheximide ml. The use of these techniques is proposed for the isolation of mycobacteria from heavily contaminated clinical specimens as well as from soil.

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1988-03-01
2021-10-23
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References

  1. Beerwerth W. 1967; Culture of mycobacteria from the droppings of domestic animals and the role of these bacteria in epizootiology. Praxis in Pneumology 21:189–202
    [Google Scholar]
  2. Beerwerth W., Schurmann J. 1969; Zur Oko- logie der Mycobakterien. Zentralblatt fur Bakterio- logie (I Abt., Orig.) 211:58–69
    [Google Scholar]
  3. Chapman J. S., Bernard J. S. 1962; The tolerances of unclassified mycobacteria. 1. Limits of the pFl tolerance. American Review of Respiratory Diseases 86:582–583
    [Google Scholar]
  4. Corper H. J., Cohn M. L. 1952; Cycloheximide (antibiotic Actidione) as a fungicide in solid media for growing tubercule bacilli from sputum. Antibiotics and Chemotherapy 2:12–15
    [Google Scholar]
  5. Corper H. J., Stoner R. E. 1946; An improved procedure for diagnostic culture of mammalian tubercle bacilli. Journal of Laboratory and Clinical Medicine 31:1364–1370
    [Google Scholar]
  6. George K. L., Falkinham J. O. III 1985; Selective medium for the isolation and enumeration of Mycobacterium avium-intracellulare and M.scrofulaceum. . Canadian Journal of Microbiology 32:10–14
    [Google Scholar]
  7. Goslee S., Wolinsky S. 1976; Water as a source of potentially pathogenic mycobacteria. American Review of Respiratory Diseases 113:287–292
    [Google Scholar]
  8. Jenkins P. A., Pattyn S. R., Portaels F. 1982; Diagnostic bacteriology. In The Biology of the Mycobacteria pp. 441–471 Ratledge C., Stanford J. L. Edited by London: Academic Press;
    [Google Scholar]
  9. Kubica G. P., Beam R. E., Palmer J. W. 1963; A method for the isolation of unclassified acid-fast bacilli from soil and water. American Review of Respiratory Diseases 88:718–720
    [Google Scholar]
  10. Merkal R. S., Richards W. D. 1972; Inhibition of fungal growth in the cultural isolation of mycobacteria. Applied Microbiology 24:205–207
    [Google Scholar]
  11. Miettinen O. S. 1976; Estimability and estimation in case referent studies. American Journal of Epidemiology 103:226–235
    [Google Scholar]
  12. Pattyn S. R., Portaels F. 1980; In vitro cultivation and characterization of Mycobacterium lepraemurium. International Journal of Leprosy 48:7–14
    [Google Scholar]
  13. Petroff S. A. 1915; A new and rapid method for the isolation and cultivation of tubercle bacilli directly from the sputum and feces. Journal of Experimental Medicine 21:38–42
    [Google Scholar]
  14. Portaels F. 1973; Contribution a l’etude des mycobacteries de l’environnement au Bas-Zaire. Annates de la Societe beige de medecine tropicale 53:373–387
    [Google Scholar]
  15. Portaels F. 1978 Etude d’Actinomycetales isolees de I'homme et de son environnement en Afrique Centrale. Doctoral thesis, University of Brussels: Belgium;
    [Google Scholar]
  16. Portaels F. 1980; Etude de mycobacteries isolees de I'homme et de son environnement au Zaire. Annales de microbiologie 131A:18–19
    [Google Scholar]
  17. Portaels F., Pattyn S. R. 1981; Parameters influencing the ‘in vitro’ growth of Mycobacterium lepraemurium. International Journal of Leprosy 49:194–197
    [Google Scholar]
  18. Portaels F., Pattyn S. R. 1982; Growth of mycobacteria in relation to the pH of the medium. Annales de microbiologie 133B:213–221
    [Google Scholar]
  19. Portaels F., Francken A., Pattyn S. R. 1982; Bacteriological studies of armadillo livers infected with Mycobacterium leprae. Annales de la Societe beige de medecine tropicale 62:233–245
    [Google Scholar]
  20. Portaels F., De ridder K., Pattyn S. R. 1985; Cultivable mycobacteria isolated from organs of armadillos uninoculated and inoculated with Mycobacterium leprae. Annales de I’lnstitut Pasteur/Microbiologie 136A:181–190
    [Google Scholar]
  21. Songer J. G. 1981; Methods for selective isolation of mycobacteria from the environment. Canadian Journal of Microbiology 27:1–7
    [Google Scholar]
  22. Thoen C. O., Himes E. M., Jarnagin J. L., Harrington R. 1979; Comparison of four culture media for isolation of Mycobacterium avium complex from porcine tissues. Journal of Clinical Microbiology 9:194–196
    [Google Scholar]
  23. Wolinsky E., Rynearson T. K. 1968; Mycobacteria in soil and their relation to diseases-associated strains. American Review of Respiratory Diseases 97:1032–1037
    [Google Scholar]
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