1887

Abstract

Vero cytotoxin (VT) production has been studied in 34 strains isolated from animals with enteric diseases. The strains were tested by DNA hybridization with probes for VT1 and VT2 sequences and also in toxin neutralization experiments with specific antisera. Twenty bovine strains, belonging to nine different O serogroups, produced VT1 or VT2 but not both toxins. In contrast, all 14 porcine strains of four different O serogroups produced VT2 only. Six of these porcine strains, belonging to serogroups 0138, 0139 and 0141, were isolated from cases of oedema disease. In general, the porcine isolates produced toxin at a lower level than the bovine strains.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-3-829
1988-03-01
2021-05-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/3/mic-134-3-829.html?itemId=/content/journal/micro/10.1099/00221287-134-3-829&mimeType=html&fmt=ahah

References

  1. Blanco J., Gonzalez E. A., Bernardez I., Regueiro B. 1983; Differentiated biological activity of Vero cytotoxin (VT) released by human and porcineEscherichia colistrains. FEMS Microbiology Letters 20:167–170
    [Google Scholar]
  2. Borczyk A. A., Karmali M. A., Lior H., Duncan L.M.C. 1987; Bovine reservoir for Verotoxin-producingEscherichia coli0157.H7. Lancet i:98
    [Google Scholar]
  3. Dobrescu L. 1983; New biological effect of edema disease principle (Escherichia colineurotoxin) and its use as an in vitro assay for this toxin. American Journal for Veterinary Research 44:31–34
    [Google Scholar]
  4. Gonzalez E. A., Blanco J. 1985; Production of cytotoxin VT in enteropathogenic and non-entero- pathogenicEscherichia colistrains of porcine origin. FEMS Microbiology Letters 26:127–130
    [Google Scholar]
  5. Gross R. J., Rowe B. 1985; Serotyping ofEscherichia coli.InThe Virulence of Escherichia coli. . Reviews and Methods pp 345–363 Sussman M. Edited by London: Academic Press;
    [Google Scholar]
  6. Gross R. J., Scotland S. M., Rowe B. 1976; Enterotoxin testing ofEscherichia colicausing epidemic infantile enteritis in the UK. Lancet i:629–631
    [Google Scholar]
  7. Hall G. A., Reynolds D. J., Chanter N., Morgan J. H., Parsons K. R., Debney T. G., Bland A. P., Bridger J. C. 1985; Dysentery caused byEscherichia coli(S102–9) in calves: natural and experimental disease. Veterinary Pathology 22:156–163
    [Google Scholar]
  8. Huang A., DeGrandis S., Friesen J., Karmali M., Petric M., Congi R., Brunton J. L. 1986; Cloning and expression of the genes specifying Shiga-like toxin production inEscherichia coliHI9. Journal of Bacteriology 106:375–379
    [Google Scholar]
  9. Karmali M. A., Steele B. T., Petric M., Lim C. 1983; Sporadic cases of haemolytic uraemic syndrome associated with faecal cytotoxin and cyto- toxin-producingEscherichia coliin stools. Lancet i:619–620
    [Google Scholar]
  10. Karmali M. A., Petric M., Lim C., Fleming P. C., Arbus G. S., Lior H. 1985; The association between idiopathic hemolytic uremic syndrome and infection by Verotoxin-producingEscherichia coli. . Journal of Infectious Diseases 151:775–782
    [Google Scholar]
  11. Karmali M. A., Petric M., Louie S., Cheung R. 1986; Antigenic heterogeneity ofEscherichia coliVerotoxins. Lancet i:164–165
    [Google Scholar]
  12. Kashiwazaki M., Ogawa T., Isayama Y., Akaike Y., Tamura K., Sakazaki R. 1980; Detection of Vero cytotoxin strains ofEscherichia coliisolated from diseased animals. National Institute of Animal Health Quarterly 20:116–117
    [Google Scholar]
  13. Kashiwazaki M., Ogawa T., Nakamura K., Iswyama T., Tamura K., Sakazaki R. 1981; Vero cytotoxin produced byEscherichia colistrains of animal origin. National Institute of Animal Health Quarterly 21:69–72
    [Google Scholar]
  14. Konowalchuk J., Speirs J. I., Stavric S. 1977; Vero response to a cytotoxin ofEscherichia coli. . Infection and Immunity 18:775–779
    [Google Scholar]
  15. Konowalchuk J., Dickie N., Stavric S., Speirs J. I. 1978; Properties of anEscherichia colicytotoxin. Infection and Immunity 20:575–577
    [Google Scholar]
  16. Marques L.R.M., Moore M. A., Wells J. G., Wachsmuth K., O’brien A. D. 1986; Production of Shiga-like toxin byEscherichia coli. . Journal of Infectious Diseases 154:338–341
    [Google Scholar]
  17. Martin M. L., Shipman L. D., Wells J. G., Putter M. E., Hedberg K., Wachsmuth I. K., Tauxe R. V., Davis J. P., Arnoldi J., Tilleli J. 1986; Isolation ofEscherichia coli0157.H7 from dairy cattle associated with two cases of haemolytic uraemic syndrome. Lancet ii:1043
    [Google Scholar]
  18. Mohammad A., Peiris J.S.M., Wijewanta E. A., Mahalingam S., Gunasekara G. 1985; Role of verocytotoxigenicEscherichia coliin cattle and buffalo calf diarrhoea. FEMS Microbiology Letters 26:281–283
    [Google Scholar]
  19. Mohammad A., Peiris J.S.M., Wijewanta E. A. 1986; Serotypes of verocytotoxigenicEscherichia coliisolated from cattle and buffalo calf diarrhoea. FEMS Microbiology Letters 35:261–265
    [Google Scholar]
  20. Newland J. W., Strockbine A. A., Miller S. F., O’Brien A. D., Holmes R. K. 1985; Cloning of Shiga-like toxin structural genes from a toxin converting phage ofEscherichia coli. . Science 230:179–181
    [Google Scholar]
  21. O’Brien A. D., Holmes R. K. 1987; Shiga and Shiga-like toxins. Microbiological Reviews 51:206–220
    [Google Scholar]
  22. O’Brien A. D., La Veck G. D., Thompson M. R., Formal S. B. 1982; Production ofShigella dysen- teriaetype 1-like cytotoxin byEscherichia coli. . Journal of Infectious Diseases 146:763–769
    [Google Scholar]
  23. O’Brien A. D., Newland J. W., Miller S. F., Holmes R. K., Williams Smith H., Formal S. B. 1984; Shiga-like toxin-converting phages fromEscherichia colistrains that cause hemorrhagic colitis or infantile diarrhoea. Science 226:694–696
    [Google Scholar]
  24. Orskov F., Orskov I., Villar J. A. 1987; Cattle as reservoir of Verotoxin-producingEscherichia coli0157.H7. Lancet ii:276
    [Google Scholar]
  25. Riley L. W., Remis R. S., Helgerson S. D., Mcgee H. B., Wells J. G., Davis B. R., Hebert R. J., Olcott E. S., Johnson L. M., Hargrett N. T., Blake P. A., Cohen M. L. 1983; Hemorrhagic colitis associated with a rareEscherichia coliserotype. New England Journal of Medicine 308:681–685
    [Google Scholar]
  26. Ryan C. A., Tauxe R. V., Hosek G. W., Wells J. G., Stoesz P. A., Mcfadden H. W., Smith P. W., Wright G. F., Blake P. A. 1986; Escherichia coli0157.H7 diarrhoea in a nursing home: clinical, epidemiological and pathological findings. Journal of Infectious Diseases 154:631–638
    [Google Scholar]
  27. Scotland S. M., Day N. P., Rowe B. 1980; Production of cytotoxin affecting Vero cells by strains ofEscherichia colibelonging to traditional enteropathogenic serogroups. FEMS Microbiology Letters 7:15–17
    [Google Scholar]
  28. Scotland S. M., Smith H. R., Willshaw G. A., Rowe B. 1983; Vero cytotoxin production in strain ofEscherichia coliis determined by genes carried on bacteriophage. Lancet ii:216
    [Google Scholar]
  29. Scotland S. M., Smith H. R., Rowe B. 1985; Two distinct toxins active on Vero cells fromEscherichia coli0157. Lancet ii:885–886
    [Google Scholar]
  30. Scotland S. M., Rowe B., Smith H. R., Willshaw G. A., Gross R. J. 1988; Vero cytotoxin- producing strains ofEscherichia colifrom children with haemolytic uraemic syndrome and their detection by specific DNA probes. Journal of Medical Microbiology 25: in the Press
    [Google Scholar]
  31. Sherwood D., Snodgrass D. R., O’Brien A. D. 1985; Shiga-like toxin production fromEscherichia coliassociated with calf diarrhoea. Veterinary Record 116:217–218
    [Google Scholar]
  32. Smith H. R., Day N. P., Scotland S. M., Gross R. J., Rowe B. 1984; Phage-determined production of Vero cytotoxin in strains ofEscherichia coliserogroup 0157. Lancet i:1242–1243
    [Google Scholar]
  33. Smith H. R., Rowe B., Gross R. J., Fry N. K., Scotland S. M. 1987; Haemorrhagic colitis and Vero cytotoxin-producingEscherichia coliin England and Wales. Lancet i:1062–1065
    [Google Scholar]
  34. Smith H. W., Green P., Parsell Z. 1983; Vero cell toxins inEscherichia coliand related bacteria: transfer by phage and conjugation and toxic action in laboratory animals, chickens and pigs. Journal of General Microbiology 129:3121–3137
    [Google Scholar]
  35. Sojka W. J. 1965; Escherichia coli in Domestic Animals and Poultry. Farnham Royal: Commonwealth Agricultural Bureaux
    [Google Scholar]
  36. Strockbine N., Marques L.R.M., Newland J. W., Smith H. W., Holmes R. K., O’Brien A. D. 1986; Two toxin-converting phages fromEscherichia coli0157.H7 strain 933 encode anti- genically distinct toxins with similar biologic activities. Infection and Immunity 53:135–140
    [Google Scholar]
  37. Willshaw G. A., Smith H. R., Scotland S. M., Rowe B. 1985; Cloning of genes determining the production of Vero cytotoxin byEscherichia coli. . Journal of General Microbiology 131:3047–3053
    [Google Scholar]
  38. Willshaw G. A., Smith H. R., Scotland S. M., Field A. M., Rowe B. 1987; Heterogeneity ofEscherichia coliphages encoding Vero cytotoxins: comparison of cloned sequences determining VT1 and VT2 and development of specific gene probes. Journal of General Microbiology 133:1309–1317
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-3-829
Loading
/content/journal/micro/10.1099/00221287-134-3-829
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error