1887

Abstract

Toluene-treated cells of HD4 used phosphoenolpyruvate (PEP) to phosphorylate glucose and sucrose. Glucose activity was constitutive, while the phosphorylation of sucrose was inducible. Competition experiments indicated that separate phosphotransferase (PTS) enzymes II were present for glucose and sucrose, but it appeared that maltose was hydrolysed by an inducible extracellular maltase and then transported by the glucose PTS. HD4 grew more slowly on maltose than glucose or sucrose and the specific activity of maltase was rate limiting. The maltase was competitively inhibited by glucose and sucrose. Xylose was not phosphorylated by PEP or ATP, and its uptake was inhibited by the protonophore carbonyl cyanide -chlorophenylhydrazone (CCCP), and by chlorhexidine diacetate. The absence of PEP-dependent phosphorylation and the effects of CCCP suggested that xylose was transported by an active transport mechanism.

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1988-03-01
2021-05-15
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References

  1. Barman T. E. 1969 Enzyme handbook 1 & 2 New York: Springer Verlag;
    [Google Scholar]
  2. Bradford M. 1976; Photometric methods for protein determination.Procedures and materials. Analytical Biochemistry 72:248–254
    [Google Scholar]
  3. Bryant M. P. 1956; The characteristics of strains of Selenomonas isolated from bovine rumen contents. Journal of Bacteriology 72:162–167
    [Google Scholar]
  4. Counotte G.H.M., Prins R. A., Janssen R.H.A.M., De Bie M.J.A. 1981; The role of Megasphaera elsdenii in the fermentation of dl-[2-14C]lactate in the rumen of dairy cattle. Applied and Environmental Microbiology 42:649–655
    [Google Scholar]
  5. Dahlqvist A. 1964; Method for assay of intestinal disaccharidases. Analytical Biochemistry 7:18–25
    [Google Scholar]
  6. Gachelin G. 1970; Studies on the α-methylglucoside permease of Escherichia coli. A two-step mechanism for the accumulation of a-methylglucoside-6-phosphate. European Journal of Biochemistry 16:342–357
    [Google Scholar]
  7. Groleau D., Forsberg C. W. 1981; Cellulolytic activity of the rumen bacterium Bacteroides succinogenes. Canadian Journal of Microbiology 27:517–530
    [Google Scholar]
  8. Harold F. M., Baarda J. R., Baron C., Abrams A. 1969; Dio 9 and chlorhexidine: inhibitors of membrane-bound ATPase and of cation transport in Streptococcus faecalis. Biochimica et biophysica acta 183:129–136
    [Google Scholar]
  9. Hobson P. N. 1965; Continuous culture of some anaerobic and facultatively anaerobic rumen bacteria. Journal of General Microbiology 38:167–180
    [Google Scholar]
  10. Hungate R. E. 1966 The Rumen and its Microbes. New York: Academic Press.;
    [Google Scholar]
  11. Keevil C. W., Williamson M. I., Marsh P. D., Ellwood D. C. 1984; Evidence that glucose and sucrose uptake in oral streptococcal bacteria involves independent phosphotransferase and protonmotive force-mediated mechanisms. Archives of Oral Biology 29:871–878
    [Google Scholar]
  12. Kesters-Hilderson H., Claeyssens M., Van Doorslaer E., Samen E., De Bruyne D. K. 1982; β-Xylosidase from Bacillus pumilus. Methods in Enzymology 83:631–639
    [Google Scholar]
  13. Kornberg H. L., Reeves R. E. 1972; Inducible phosphoenolpyruvate-dependent hexose phosphotransferase activities in Escherichia coli. Biochemical Journal 128:1339–1344
    [Google Scholar]
  14. Lam V.M.S., Daruwalla K. R., Henderson P.J.F., Jones-Mortimer M. C. 1980; Proton-linked d-xylose transport in Escherichia coli. Journal of Bacteriology 143:396–402
    [Google Scholar]
  15. Latham M. J., Sharpe M. E., Sutton J. D. 1971; The microbial flora of the rumen of cows fed hay and high cereal rations and its relationship to the rumen fermentation. Journal of Applied Bacteriology 34:425–434
    [Google Scholar]
  16. Lessel E. R. JR Breed R. S. 1954; Selenomonas Boskamp, 1922 - a genus that includes species showing an unusual type of flagellation. Bacteriological Reviews 18:165–169
    [Google Scholar]
  17. London J., Chase N. M. 1977; New pathway for the metabolism of pentitols. Proceedings of the National Academy of Sciences of the United States of America 74:4296–4300
    [Google Scholar]
  18. London J., Chase N. M. 1979; Pentitol metabolism in Lactobacillus casei. Journal of Bacteriology 140:949–954
    [Google Scholar]
  19. London J., Hausman S. 1982; Xylitol-mediated transient inhibition of ribitol utilization by Lactobacillus casei. Journal of Bacteriology 150:657–661
    [Google Scholar]
  20. Marsh P. D., Keevil C. W., Mcdermid A. S., Williamson M. I., Ellwood D. C. 1983; Inhibition by the antimicrobial agent chlorhexidine of acid production and sugar transport in oral streptococcal bacteria. Archives of Oral Biology 28:233–240
    [Google Scholar]
  21. Marsh P. D., Keevil C. W., Ellwood D. C. 1984; Relationship of bioenergetic processes to the pathogenic properties of oral bacteria. Journal oj Dental Research 63:401–406
    [Google Scholar]
  22. Martin S. A., Russell J. B. 1986; Phosphoenolpyruvate-dependent phosphorylation of hexoses by ruminal bacteria: evidence for the phosphotransferase transport system. Applied and Environmental Microbiology 52:1348–1352
    [Google Scholar]
  23. Martin S. A., Russell J. B. 1987; Transport and phosphorylation of disaccharides by the ruminal bacterium Streptococcus bovis. Applied and Environmental Microbiology 53:2388–2393
    [Google Scholar]
  24. Matin A., Veldkamp H. 1978; Physiological basis of the selective advantage of a Spirillum sp. in a carbon-limited environment. Journal of General Microbiology 105:187–197
    [Google Scholar]
  25. Postma P. W., Lengeler J. W. 1985; Phospho- enolpyruvate : carbohydrate phosphotransferase system of bacteria. Microbiological Reviews 49:232–269
    [Google Scholar]
  26. Robinson I. M., Allison M. J., Buchlin J. A. 1981; Characterization of the cecal bacteria of normal pigs. Applied and Environmental Microbiology 41:950–955
    [Google Scholar]
  27. Romano A. H., Trifone J. D., Brustolon M. 1979; Distribution of the phosphoenolpyruvate: glucose phosphotransferase system in fermentative bacteria. Journal of Bacteriology 139:93–97
    [Google Scholar]
  28. Russell J. B., Baldwin R. L. 1978; Substrate preferences in rumen bacteria: evidence of catabo- lite regulatory mechanisms. Applied and Environmental Microbiology 36:319–329
    [Google Scholar]
  29. Russell J. B., Baldwin R. L. 1979; Comparison of substrate affinities among several rumen bacteria: a possible determinant of rumen bacterial competition. Applied and Environmental Microbiology 37:531–536
    [Google Scholar]
  30. Russell J. B., Dombrowski D. B. 1980; Effect of pH on the efficiency of growth by pure cultures of rumen bacteria in continuous culture. Applied and Environmental Microbiology 39:604–610
    [Google Scholar]
  31. Saier M. H. JR 1985 Mechanisms and Regulation of Carbohydrate Transport in Bacteria. New York:: Academic Press.;
    [Google Scholar]
  32. Scheifinger C. C., Latham M. J., Wolin M. J. 1975; Relationship of lactate dehydrogenase specificity and growth rate to lactate metabolism by Selenomonas ruminantium. Applied and Environmental Microbiology 30:916–921
    [Google Scholar]
  33. Segel I. H. 1976 Biochemical Calculations,, 2nd edn.. pp. 248–252 New York: John Wiley;
    [Google Scholar]
  34. Sissons A., Midgley M. 1981; Energy transduction in Chlorobiumlimicola : role of membrane-bound adenosine triphosphatase and the proton electrochemical gradient. Journal of General Microbiology 122:211–216
    [Google Scholar]
  35. St Martin E. J., Wittenberger C. L. 1979; Characterization of a phosphoenolpyruvate-dependent sucrose phosphotransferase system in Streptococcus mutans. Infection and Immunity 24:865–868
    [Google Scholar]
  36. Vadeboncoeur C., Trahan L. 1982; Glucose transport in Streptococcus salivarius. Evidence for the presence of a distinct phosphoenolpyruvate : glucose phosphotransferase system which catalyses the phosphorylation of α-methylglucoside. Canadian Journal of Microbiology 28:190–199
    [Google Scholar]
  37. Vadeboncoeur C., Proulx M., Trahan L. 1983; Purification of proteins similar to HPr and enzyme I from the oral bacterium Streptococcus salivarius.Biochemical and immunochemical properties. Canadian Journal of Microbiology 29:1694–1705
    [Google Scholar]
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