RT Journal Article SR Electronic(1) A1 Van doorn, Jaap A1 Scholte, Marcel E. A1 Postma, Pieter W. A1 Van driel, ROEL A1 Van dam, KarelYR 1988 T1 Regulation of Trehalase Activity during the Cell Cycle of Saccharomyces cerevisiae JF Microbiology, VO 134 IS 3 SP 785 OP 790 DO https://doi.org/10.1099/00221287-134-3-785 PB Microbiology Society, SN 1465-2080, AB Synchronous cultures of Saccharomyces cerevisiae prepared by selection of small unbudded cells from an elutriating rotor were used to measure trehalase activity during the cell cycle. After the small cells had been removed from the rotor, the remainder was used to prepare asynchronous control cultures. Both synchronous and control cultures were studied for two cell cycles. In asynchronous cultures the trehalase activity of crude cell lysates rose continuously. In synchronized populations trehalase activity increased from the beginning of budding onwards. However, around the period of cell division the enzyme activity dropped rapidly but transiently by more than 5-fold. The same changes were found during the second budding cycle. Measurements of invertase and glucose-6-phosphate dehydrogenase activities in the same synchronous and asynchronous cultures revealed a continuous increase for both enzymes. Incubation of cell lysates with cAMP-dependent protein kinase before assaying for trehalase resulted in a 2-fold enhancement of enzyme activity in asynchronous control cultures. In synchronized cells this treatment also led to a significant stimulation of trehalase activity, and largely abolished the cell-cycle-dependent oscillatory pattern of enzyme activity. These results suggest that the activity of trehalase during the cell cycle is regulated, presumably at the post-translational level, by a phosphorylation-dephosphorylation mechanism., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-134-3-785