1887

Abstract

We report the nucleotide sequence of a 1·45 kb segment containing the gene, coding for staphylococcal exfoliative toxin A (ETA), isolated from the recombinant plasmid pETA-J3. The coding region of 840 bp specified a polypeptide of 280 amino acid residues which included a putative 38 residue signal sequence. The amino acid composition deduced from the structural gene was in agreement with the results of peptide analysis of the ETA molecule reported by others. The sequence of the 35 N-terminal amino acid residues of ETA derived from strain ZM was also consistent with that deduced from the DNA sequencing.

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1988-03-01
2021-08-05
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References

  1. Arbuthnott J. P., Kent J., Lyell A., Gemmell C. G. 1971; Toxic epidermal necrolysis produced by an extracellular product of Staphylococcus aureus. . British Journal of Dermatology 85:145–149
    [Google Scholar]
  2. Hawley D. K., Mcclure W. R. 1983; Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Research 11:2237–2255
    [Google Scholar]
  3. Jackson M. P., Iandolo J. J. 1986; Sequence of the exfoliative toxin B gene of Staphylococcus aureus. . Journal of Bacteriology 166:574–580
    [Google Scholar]
  4. Johnson A. D., Spero L., Cades J. S., De Cicco B. T. 1979; Purification and characterization of different types of exfoliative toxin from Staphylococcus aureus. . Infection and Immunity 24:679–684
    [Google Scholar]
  5. Kapral F. A., Miller M. M. 1971; Product of Staphylococcus aureus responsible for the scalded skin syndrome. Infection and Immunity 4:541–545
    [Google Scholar]
  6. Kondo I., Sakurai S. 1980; Studies on toxicity and immunology of staphylococcal exfoliatin A and B. In Natural Toxins pp 379–387 Eaker D., Wadström T. Edited by Oxford & New York: Pergamon Press;
    [Google Scholar]
  7. Kondo I., Sakurai S., Sarai Y. 1973; Purification of exfoliatin produced by Staphylococcus aureus of bacteriophage group 2 and its physicochemical properties. Infection and Immunity 8:156–164
    [Google Scholar]
  8. Kondo I., Sakurai S., Sarai Y. 1974; New type of exfoliatin obtained from staphylococcal strains, belonging to phage groups other than group 2, isolated from patients with impetigo and Ritter’s disease. Infection and Immunity 10:851–861
    [Google Scholar]
  9. Kondo I., Sakurai S., Sarai Y., Futaki S. 1975; Two types of exfoliatin and their distribution in staphylococcal strains isolated from patients with scalded skin syndrome. Journal of Clinical Microbiology 1:397–400
    [Google Scholar]
  10. Kyte J., Doolittle F. R. 1971; Product of Staphylococcus aureus responsible for the scalded skin syndrome. Infection and Immunity 4:541–545
    [Google Scholar]
  11. Lee C. Y., Schmidt J. J., Johnson-Winger A. D., Spero L., Iandolo J. J. 1987; Sequence determination and comparison of the exfoliative toxin A and toxin B cloned from Staphylococcus aureus. . Journal of Bacteriology 169:3904–3909
    [Google Scholar]
  12. Melish M. F., Glasgow L. A. 1970; The staphylococcal scalded skin syndrome: development of an experimental model. New England Journal of Medicine 282:1114–1119
    [Google Scholar]
  13. Messing J., Vieira J. 1982; A new pair of Ml3 vectors for selecting either DNA strand of doubledigest restriction fragments. Gene 19:269–276
    [Google Scholar]
  14. Miller J. H. editor 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. O’Toole L. L., Foster T. J. 1986a; Epidermolytic toxin serotype B of Staphylococcus aureus is plasmidencoded. FEMS Microbiology Letters 36:311–314
    [Google Scholar]
  16. O’Toole L. L., Foster T. J. 1986b; Molecular cloning and expression of epidermolytic toxin A gene of Staphylococcus aureus. . Microbial Pathogenesis 1:583–594
    [Google Scholar]
  17. Rogolsky M., Warren R., Wiley B. B., Nakamura H. T., Glasgow L. A. 1974; Nature of the genetic determinant controlling exfoliative toxin production in Staphylococcus aureus. . Journal of Bacteriology 117:157–165
    [Google Scholar]
  18. Rogolsky M., Wiley B. B., Glasgow L. A. 1976; Phage group 2 staphylococcal strains with chromosomal and extrachromosomal genes for exfoliative toxin production. Infection and Immunity 13:44–52
    [Google Scholar]
  19. Rosenberg M., Court D. 1979; Regulatory sequences involved in the promotion and termina-tion of RN A transcription. Annual Review of Genetics 13:319–353
    [Google Scholar]
  20. Sakurai S., Suzuki H., Kondo I. 1987; Cloning of the gene coding for staphylococcal exfoliative toxin A and its expression in Escherichia coli. . FEMS Microbiology Letters 42:63–67
    [Google Scholar]
  21. Sanger F. 1981; Determination of nucleotide se-quences in DNA. Science 214:1205–1210
    [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  23. Sanger F., Coulson A. R., Barvell B. G., Smith A.J.H., Roe B. A. 1980; Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Determination of nucleotide sequences in DNA. Science 214:1205–1210
    [Google Scholar]
  24. Tarr G. E. 1986; A practical manual. In Microcharacterization of Polypeptides pp 155–194 Shively J. E. Edited by Clifton, NJ: Humana Press;
    [Google Scholar]
  25. Uhlen M., Guss B., Nilsson B., Gatenbeck S., Philipson L., Lindberg M. 1983; Complete sequence of the staphylococcal gene encoding protein A - a gene evolved through multiple duplica-tions. Journal of Biological Chemistry 259:1695–1702
    [Google Scholar]
  26. Wiley B. B., Rogolsky M. 1977; Molecular and serological differentiation of staphylococcal exfoliative toxin synthesized under chromosomal and plasmid control. Infection and Immunity 18:487–494
    [Google Scholar]
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