1887

Abstract

Transfer of growing cells of the salt-tolerant yeast to media of higher salinity resulted in an increased production and intracellular accumulation of glycerol, which was proportional to the magnitude of the shift in salinity. Stress solutes other than NaCl, when added in iso-osmolar concentrations, promoted the accumulation of similar amounts of glycerol. Cells grown at high salinity rapidly lost glycerol when returned to media of lower salinity and the loss was greater when the cells were transferred to more dilute media. A mutant strain of showed poor glycerol production and was inhibited by NaCl at concentrations about half the maximum tolerated by the wild-type. Growth of this mutant occurred at otherwise inhibitory NaCl concentrations if the medium was supplemented with a low concentration of glycerol. The added glycerol was intracellularly accumulated to levels that increased with salinity and were only slightly lower than the corresponding wild-type levels. Glycerol additions above the growth promoting level had little effect on growth rate but caused substantial shortening of the lag phase. Osmoprotectants other than glycerol did not permit growth to occur. The mutant was isolated as a glycerol non-utilizer but displayed growth in glycerol media at increased NaCl concentrations.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-3-669
1988-03-01
2021-08-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/3/mic-134-3-669.html?itemId=/content/journal/micro/10.1099/00221287-134-3-669&mimeType=html&fmt=ahah

References

  1. Adler L. 1986; Physiological and biochemical characteristics of the yeast Debaryomyce shansenii in relation to salinity. In The Biology of Marine Fungi pp. 81–91 Moss T. Edited by Cambridge, UK: Cambridge University Press;
    [Google Scholar]
  2. Adler L., Gustafsson L. 1980; Polyhydric alcohol production and intracellular amino acid pool in relation to halotolerance of the yeast Debaryomyces hansenii. Archives of Microbiology 124:123–130
    [Google Scholar]
  3. Adler L., Falk K.-E., Norkrans B., Ångström J. 1981; Polyol and water in the salt-tolerant yeast Debaryomyces hansenii as studied by 1H nuclear magnetic resonance. FEMS Microbiology Letters 11:269–271
    [Google Scholar]
  4. Adler L., Blomberg A., Nilsson A. 1985; Glycerol metabolism and osmoregulation in the salttolerant yeast Debaryomyces hansenii. Journal of Bacteriology 162:300–306
    [Google Scholar]
  5. Brown A. D. 1974; Microbial water relations: features of the intracellular composition of sugar-tolerant yeasts. Journal of Bacteriology 118:769–777
    [Google Scholar]
  6. Brown A. D. 1976; Microbial water stress. Bacteriological Reviews 40:803–846
    [Google Scholar]
  7. Brown A. D. 1978; Compatible solutes and extreme water stress in eucaryotic micro-organisms. Advances in Microbial Physiology 17:181–242
    [Google Scholar]
  8. Cole M. B., Keenan M. H. J. 1987; Effects of weak acids and external pH on the intracellular pH of Zygosaccharomyces bailii, and its implications in weak-acid resistance. Yeast 3:23–32
    [Google Scholar]
  9. Gould G. W., Measures J. C. 1977; Water relations in single cells. Philosophical Transactions of the Royal Society B278:151–166
    [Google Scholar]
  10. Gustafsson L. 1979; The ATP pool in relation to the production of glycerol and heat during growth of the halotolerant yeast Debaryomyces hansenii. Archives of Microbiology 120:15–23
    [Google Scholar]
  11. Gustafsson L., Norkrans B. 1976; On the mechanism of salt tolerance.Production of glycerol and heat during growth of Debaryomyces hansenii. Archives of Microbiology 110:177–183
    [Google Scholar]
  12. Harris R. F. 1981; Effect of water potential on microbial growth and activity. In Water Potential Relations in Soil Microbiology (Soil Science Society of America Special Publication no. 9 pp. 23–95 Parr J. F., Gardner W. R., Elliott L. F. Edited by Madison, Wisconsin, USA: Soil Science Society of America;
    [Google Scholar]
  13. Hobot J. A., Jennings D. H. 1981; Growth of Debaryomyces hansenii and Saccharomyces cerevisiae in relation to pH and salinity. Experimental Mycology 5:217–228
    [Google Scholar]
  14. Laimins L. A., Rhoads D. B., Epstein W. 1981; Osmotic control of kdp operon expression in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 78:464–468
    [Google Scholar]
  15. Lord P. G., Wheals A. E. 1980; Asymmetrical division of Saccharomyces cerevisiae. Journal of Bacteriology 142:808–818
    [Google Scholar]
  16. Meredith S. A., Romano A. H. 1977; Uptake and phosphorylation of 2-deoxy-d-glucose by wildtype and respiration-deficient bakers’ yeast. Biochimica et biophysica acta 497:745–759
    [Google Scholar]
  17. Nobre F. M., Dacosta M. S. 1985; The accumulation of polyols by the yeast Debaryomyces hansenii in response to water stress. Canadian Journal of Microbiology 31:1061–1064
    [Google Scholar]
  18. Norkrans B. 1966; Studies on marine occurring yeasts: Growth related to pH, NaCl concentration and temperature. Archiv für Mikrobiologie 54:374–392
    [Google Scholar]
  19. Norkrans B., Kylin A. 1969; Regulation of the potassium to sodium ratio and of the osmotic potential in relation to salt tolerance in yeast. Journal of Bacteriology 100:836–845
    [Google Scholar]
  20. Onishi H. 1963; Osmophilic yeasts. Advances in Food Research 12:53–94
    [Google Scholar]
  21. Sutherland L., Cairney J., Elmore M. J., Booth I. R., Higgins C. F. 1986; Osmotic regulation of transcription: induction of the proU betaine transport gene is dependent on accumulation of intracellular potassium. Journal of Bacteriology 168:805–814
    [Google Scholar]
  22. Yancey P. H., Clark M. E., Hand S. C., Bowlus R. D., Somero G. N. 1982; Living with water stress: evoluton of osmolyte systems. Science 217:1214–1222
    [Google Scholar]
  23. Wickerham L. J. 1951; Taxonomy of yeasts. US Department of Agriculture Technical Bulletin no. 1029 pp. 1–51
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-3-669
Loading
/content/journal/micro/10.1099/00221287-134-3-669
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error