SUMMARY: The origin of aflatoxin G was studied using mutant strains of blocked early in the pathway and by tracing C-labelled aflatoxin B (AFB) in wild-type and strains. Sterigmatocystin (ST) was a precursor of AFB, AFG and AFG in the four mutants examined. The identity of AFG was confirmed by mass spectrometry. No evidence for conversion of AFB to AFG was found. A rigorously controlled study of conversions of radioactivity based on preparative thin-layer chromatography of aflatoxins demonstrated that low levels of aflatoxin interconversions previously reported in the literature might actually be artifacts.


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