@article{mbs:/content/journal/micro/10.1099/00221287-134-3-635, author = "Lee, Yuan-kun and Tan, Hai-meng", title = "Interphylum Protoplast Fusion and Genetic Recombination of the Algae Porphyridium cruentum and Dunaliella spp", journal= "Microbiology", year = "1988", volume = "134", number = "3", pages = "635-641", doi = "https://doi.org/10.1099/00221287-134-3-635", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-134-3-635", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "True protoplasts of the red alga Porphyridium cruentum and the halophilic green algae Dunaliella bardawil and Dunaliella salina were induced by treatment with pectinase and cellulysin. Protoplasts of P. cruentum and the Dunaliella spp. were fused by polyethylene glycol treatment and then allowed to regenerate on selection media. Fusants that were essentially Porphyridium in nature were generated between P. cruentum and D. bardawil and D. salina; these tolerated a salinity of up to 3·5 m for the former and 2·5 m for the latter, indicating that they were hybrid strains, acquiring the osmotolerance from the Dunaliella spp. A Dunaliella-like clone was isolated from protoplast fusion between P. cruentum and D. salina, with altered antibiotic sensitivity. The acquired resistance to penicillin and erythromycin appears to be the result of a genetic transfer from P. cruentum. ", }