RT Journal Article SR Electronic(1) A1 Leonhardt, Heinrich A1 Alonso, Juan C.YR 1988 T1 Construction of a Shuttle Vector for Inducible Gene Expression in Escherichia coli and Bacillus subtilis JF Microbiology, VO 134 IS 3 SP 605 OP 609 DO https://doi.org/10.1099/00221287-134-3-605 PB Microbiology Society, SN 1465-2080, AB The construction of a shuttle vector for inducible gene expression allowing fast and easy cloning in Escherichia coli and subsequent transformation of Bacillus subtilis is presented. The expression is based on the regulation of the tac promoter by the Lac repressor which was assayed with the xylE gene from Pseudomonas putida as a marker gene. The laclq gene, transcribed by the strong spo promoter, allowed full repression of the weak tac promoter., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-134-3-605