1887

Abstract

Determinants of tetracycline resistance have been cloned from two different tetracycline-producing industrial strains of into using the plasmid vector pUT206. Three plasmids, pUT250 and pUT260 with a 9·5 and a 7·5 kb insert respectively of DNA, and pUT270 with a 14·0 kb insert of DNA, conferring resistance to tetracycline, have been isolated. By sub-cloning, a similar fragment of 2·45 kb containing the tetracycline resistance gene () was further localized on these plasmids. The gene has been cloned into and expressed under the control of λpL or Lpp promoters. Differential protein extraction of cells revealed the presence of an additional membrane-embedded protein in tetracycline-resistant cells. On the basis of available restriction endonuclease maps, the 347 gene is probably identical to the gene from recently identified by T. Ohnuki and co-workers as responsible for the reduced accumulation of tetracycline. The nucleotide sequence of a 2052 bp DNA fragment containing the Tc structural gene from has been determined. The amino acid sequence of the 347 protein ( 35818) deduced from the nucleotide sequence shows a limited but significant homology to other characterized tetracycline transport acting determinants from pathogenic bacteria.

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1988-03-01
2022-01-22
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References

  1. Berdy J. 1974; Recent developments of antibiotic research and classification of antibiotics according to chemical structure. Advances in Applied Microbiology 18:309–406
    [Google Scholar]
  2. Bernan V., Filpula D., Herbor W., Bibb M., Katz E. 1985; The nucleotide sequence of the tyrosinase gene from Streptomyces antibioticus and characterisation of the gene product. Gene 37:101–110
    [Google Scholar]
  3. Bibb M. J., Cohen S. N. 1982; Gene expression in Streptomyces: construction and application of promoter-probe plasmid vectors in Streptomyces lividans. Molecular and General Genetics 187:265–277
    [Google Scholar]
  4. Bibb M. J., Findlay P. R., Johnson M. W. 1984; The relationship between base composition and codon usage of bacterial genes and its use for the simple and reliable identification of protein-coding sequences. Gene 30:157–166
    [Google Scholar]
  5. Bibb M. J., Bibb M. J., Ward J. M., Cohen S. N. 1985; Nucleotide sequence encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces. Molecular and General Genetics 199:26–36
    [Google Scholar]
  6. Burdett V., Inamine J., Rajagopalan S. 1982; Heterogeneity of tetracycline resistance determinants in Streptococcus. Journal of Bacteriology 149:995–1004
    [Google Scholar]
  7. Buttner M. J., Brown N. L. 1987; Two promoters from the Streptomyces plasmid pi J101 and their expression in Escherichia coli. Gene 51:179–186
    [Google Scholar]
  8. Chater K. F., Bruton C. J. 1985; Resistance, regulatory and production genes for the antibiotic methylenomycin are clustered. EMBO Journal 4:1893–1897
    [Google Scholar]
  9. Chater K. F., Hopwood D. A., Kieser T., Thompson C. J. 1982; Gene cloning in Streptomyces. Current Topics in Microbiology and Immunology 96:69–95
    [Google Scholar]
  10. Crameri R., Davies J. E. 1986; Increased production of aminoglycosides associated with amplified antibiotic resistance genes. Journal of Antibiotics 39:128–135
    [Google Scholar]
  11. Davies J. 1986; A new look at antibiotic resistance. FEMS Microbiology Reviews 39:128–135
    [Google Scholar]
  12. Demain A. L. 1974; How do antibiotic-producing microorganisms avoid suicide?. Annals of the New York Academy of Sciences 235:601–612
    [Google Scholar]
  13. Deng Z., Kieser T., Hopwood D. A. 1987; Activity of a Streptomyces transcriptional terminator in Escherichia coli. Nucleic Acids Research 15:2665–2675
    [Google Scholar]
  14. Distler J., Piepersberg W. 1985; Cloning and characterization of a gene from Streptomyces griseuscoding for a streptomycin-phosphorylating activity. FEMS Microbiology Letters 28:113–117
    [Google Scholar]
  15. Drocourt D., Tiraby G. 1983; A survey of plasmids among natural isolates of Streptomyces. Journal of Antibiotics 36:181–184
    [Google Scholar]
  16. Hanahan D. 1985; Techniques for transformation of E. coli. In DNA Cloning: a Practical Approach I pp 109–135 Glover D. M. Edited by Oxford: IRL Press;
    [Google Scholar]
  17. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985 Genetic Manipulation of Streptomyces, a Laboratory Manual Norwich:: John Innes Foundation;
    [Google Scholar]
  18. Horinouchi S., Suzuki H., Beppu T. 1986; Nucleotide sequence of afsB, a pleiotropic gene involved in secondary metabolism in Streptomyces coelicolor A3(2) and Streptomyces lividans. Journal of Bacteriology 168:257–269
    [Google Scholar]
  19. Hoshino T., Ikeda T., Tomizuka N., Furukawa K. 1985; Nucleotide sequence of the tetracycline resistance gene of pTHT15, a thermophilicBacillus plasmid: comparison with staphylococcal TcR controls. Gene 37:131–138
    [Google Scholar]
  20. Janssen G. R., Bibb M. J., Smith C. P., Ward J. M., Kieser T., Bibb M. J. 1985; Isolation and analysis of Streptomyces promotors. In Microbiology -1985, pp. 392–396 Hegemon G., Hershberger C. L. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  21. Katz E., Thompson C. J., Hopwood D. A. 1983; Cloning and expression of the tyrosinase gene from Streptomyces antibioticusIn Streptomyces lividans. Journal of General Microbiology 129:2703–2714
    [Google Scholar]
  22. Khan S. A., Novick R. P. 1983; Complete nucleotide sequence of pT181, a tetracycline resistance plasmid from Staphylococcus aureus. Plasmid 10:251–259
    [Google Scholar]
  23. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  24. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Marcel T., Drocourt D., Tiraby G. 1987; Cloning of glucose-isomerase (D-xyluloseisomerase) and xylulose kinase genes of Streptomyces violaceoniger. Molecular and General Genetics 208:121–126
    [Google Scholar]
  26. Marshall B., Morrissey S., Flynn P., Levy S. B. 1986; A new tetracycline-resistance determinant, class E, isolated from Enterobacteriaceae. Gene 50:111–117
    [Google Scholar]
  27. Martin P., Trieu-Cuot P., Courvalin P. 1986; Nucleotide sequence of the tetM tetracycline resistance determinant of the streptococcal conjugative shuttle transposon Tn1545. Nucleic Acids Research 14:7047–7058
    [Google Scholar]
  28. Masui Y., Coleman J., Inouye M. 1983; Multipurpose expression cloning vehicles in Escherichia coli. In Experimental Manipulation of Gene Expression pp 15–32 Inouye M. Edited by New York: Academic Press;
    [Google Scholar]
  29. Murakami T., Anzai H., Imai S., Satoh A., Nagaoka K., Thompson C. J. 1986; The bialaphos biosynthetic genes of Streptomyces hygroscopicus: molecular cloning and characterization of the gene cluster. Molecular and General Genetics 205:42–50
    [Google Scholar]
  30. Neu H. C., Heppel L. A. 1965; The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. Journal of Biological Chemistry 240:3685–3692
    [Google Scholar]
  31. Nguyen T. T., Postle K., Bertrand K. P. 1983; Sequence homology between the tetracycline-resistance determinants of Tn1O and pBR322. Gene 25:83–92
    [Google Scholar]
  32. Norrander J., Kempe T., Messing J. 1983; Construction of improved Ml3 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26:101–106
    [Google Scholar]
  33. Ohnuki T., Katch T., Imanaka T., Aiba S. 1985a; Molecular cloning of tetracyline resistance genes from Streptomyces rimosusIn Streptomyces griseus and characterization of the cloned genes. Journal of Bacteriology 161:1010–1016
    [Google Scholar]
  34. Ohnuki T., Imanaka T., Aiba S. 1985b; Selfcloning in Streptomyces griseus of an str gene cluster for streptomycin biosynthesis and streptomycin resistance. Journal of Bacteriology 164:85–94
    [Google Scholar]
  35. Okanishi M., Suzuki K., Umezawa H. 1974; Formation and reversion of Streptomyces protoplasts : cultural conditions and morphological study. Journal of General Microbiology 80:389–400
    [Google Scholar]
  36. Peden K. W. C. 1983; Revised sequence of the tetracycline-resistance gene of pBR322. Gene 22:277–280
    [Google Scholar]
  37. Rhodes P. M., Hunter I. S., Friend E. J., Warren M. 1984; Recombinant DNA methods for the oxytetracycline producer Streptomyces rimosus. Biochemical Society Transactions 12:586–587
    [Google Scholar]
  38. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  39. Stanzak R., Matsushima P., Baltz R. H., Rao R. N. 1986; Cloning and expression in Streptomyces lividans of clustered erythromycin biosynthesis genes from Streptomyces erythreus. Bio/Technology 4:229–232
    [Google Scholar]
  40. Thompson C. J., Gray G. S. 1983; Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phospho-tranferases encoded by resistance plasmids. Proceedings of the National Academy of Sciences of the United States of America 80:5190–5194
    [Google Scholar]
  41. Thompson C. J., Kieser T., Ward J. M., Hopwood D. A. 1982; Physical analysis of antibiotic-resistance genes from Streptomyces and their use in vector construction. Gene 20:51–62
    [Google Scholar]
  42. Uchiyama H., Weisblum B. 1985; AT-Methyl transferase of Streptomyces erythraeus that confers resistance to the macrolide-lincosamine-strepto-gramin B antibiotics: amino acid sequence and its homology to cognate R-factor enzymes from pathogenic bacilli and cocci. Gene 38:103–110
    [Google Scholar]
  43. Vining L. C. 1979; Antibiotic tolerance in producer organisms. Advances in Applied Microbiology 25:147–168
    [Google Scholar]
  44. Waters S. H., Rogowsky P., Grinsted J., Alten-Buchner J., Schmitt R. 1983; The tetracycline resistance determinants of RP1 and Tnl721: nucleotide sequence analysis. Nucleic Acids Research 11:6089–6105
    [Google Scholar]
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