1887

Abstract

Three extracellular proteases produced by during growth in liquid medium were examined for their effects on human α-1-antitrypsin (α-1-AT). One of these proteases, tissue-destructive protease (TDP) destroyed completely the trypsin-inhibitory capacity of α-1-AT at protease: inhibitor molar ratios down to 0.002:1. After inactivation by TDP, the of α-1-AT was reduced by 5000 in SDS-PAGE. This suggested that inactivation entailed only limited cleavage.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-2-481
1988-02-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/2/mic-134-2-481.html?itemId=/content/journal/micro/10.1099/00221287-134-2-481&mimeType=html&fmt=ahah

References

  1. Abrams W.R., Fein A.M., Kucich U., Kueppers F., Yamada H., Kuzmowycz T., Morgan L., Lippmann M., Goldberg S.K., Weinbaum G. 1984; Proteinase inhibitory function in inflammatory lung disease. American Review of Respiratory Disease 129:735–741
    [Google Scholar]
  2. Baskerville A., Conlan J.W., Ashworth L.A.E., Dowsett A.B. 1986; Pulmonary damage caused by a protease from Legionella pneumophila. British Journal of Experimental Pathology 67:527–536
    [Google Scholar]
  3. Blackmon J.A., Chandler F.W., Cherry W.B., England A.C., Feeley J.C., Hicklin M.D., Mckinney R.M., Wilkinson H.W. 1981; Legionellosis. American Journal of Pathology 103:429–465
    [Google Scholar]
  4. Conlan J.W., Baskerville A., Ashworth L.A.E. 1986; Separation of Legionella pneumophila proteases and purification of a protease which produces lesions like those of Legionnaires’ disease in guinea pig lung. Journal of General Microbiology 132:1565–1574
    [Google Scholar]
  5. Conlan J.W. 1987 Studies of lipopolysaccharide and extracellular proteases from Legionellae of differing virulence PhD thesis, PHLS, CAMR, Porton Down, Salisbury, UK (CNAA)
    [Google Scholar]
  6. Dreyfus L.A., Iglewski B.H. 1986; Purification and characterisation of an extracellular protease of Legionella pneumophila. Infection and Immunity 51:736–743
    [Google Scholar]
  7. Edelstein P.H. 1981; Improved semi-selective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. Journal of Clinical Microbiology 14:298–303
    [Google Scholar]
  8. Gadek J.E., Fells G.A., Crystal R. 1979; Cigarette smoke induces functional antiproteinase deficiency in the lower respiratory tract of humans. Science 206:1316–1326
    [Google Scholar]
  9. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–684
    [Google Scholar]
  10. Molla A., Matsumoto K., Oyamada I., Katsuki T., Maeda H. 1986; Degradation of protease inhibitors, immunoglobulins and other serum proteins by Serratia protease and its toxicity to fibroblasts in culture. Infection and Immunity 53:522–529
    [Google Scholar]
  11. Morihara K., Tsuzuki H., Oda K. 1979; Protease and elastase of Pseudomonas aeruginosa: inactivation of human plasma α-1-proteinase inhibitor. Infection and Immunity 24:188–193
    [Google Scholar]
  12. Möller H.E. 1983; Induction of proteinases in Legionella by growth on agar containing serum. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene 256:211–221
    [Google Scholar]
  13. Ristroph J.D., Hedlund K.W., Allen R.G. 1980; Liquid medium for growth of Legionella pneumophila. Journal of Clinical Microbiology 11:19–21
    [Google Scholar]
  14. Travis J., Johnson D. 1981; Human α-1-proteinase inhibitor. Methods in Enzymology 80:754–765
    [Google Scholar]
  15. Williams A., Baskerville A., Dowsett A.B., Conlan J.W. 1987; Immunocytochemical demonstration of the association between L.pneumophila, its tissue-destructive protease and pulmonary lesions in experimental Legionnaires’ disease. Journal of Pathology 53: (in the Press)
    [Google Scholar]
  16. Winn W.C., Myerowitz R.L. 1981; The pathology of the Legionella pneumonias. Human Pathology 12:401–422
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-2-481
Loading
/content/journal/micro/10.1099/00221287-134-2-481
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error