1887

Abstract

Humoral responses to proteins, especially the 168 kDa protein, were demonstrated by Western blotting in sera and bronchial washings of all groups of infected or immunized guinea-pigs. However, infection was not prevented by these local and systemic antibodies. Hilar lymphocytes of infected and immunized guinea-pigs were stimulated by sonicated antigen and by the 168 kDa protein. Stimulation was significantly lower in animals which had been infected twice or had been preimmunized and challenged by infection. Histologically the most severe lesions were seen in the twice-infected group followed by the preimmunized group which was subsequently infected.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-2-473
1988-02-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/2/mic-134-2-473.html?itemId=/content/journal/micro/10.1099/00221287-134-2-473&mimeType=html&fmt=ahah

References

  1. Biberfeld G., Sterner G. 1976; Effect of Mycoplasma pneumoniae infection on cell-mediated immunity. Infection 4:17–20
    [Google Scholar]
  2. Brunner H., Feldner J., Bredt W. 1984; Effect of monoclonal antibodies to the attachment-tip on experimental Mycoplasma pneumoniae infection of hamsters: a preliminary report. Israel Journal of Medical Sciences 20:878–881
    [Google Scholar]
  3. Dajani A.S., Clyde W.A., Denny F.W.A. 1965; Experimental infection with Mycoplasma pneumoniae. Journal of Experimental Medicine 121:1071–1085
    [Google Scholar]
  4. Feldner J., Göbel U., Bredt W. 1982; Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody. Nature, London 298:765–769
    [Google Scholar]
  5. Fernald G.W. 1979; Humoral and cellular immune responses to mycoplasmas. In The Mycoplasmas II pp. 399–423 Edited by Tully J. G., Whitcomb R. F. New York: Academic Press;
    [Google Scholar]
  6. Hayflick L. 1965; Cell cultures and mycoplasmas. Texas Reports on Biology and Medicine 23: Suppl 285–303
    [Google Scholar]
  7. Hu P.C., Cole R.M., Huang Y.S., Graham J.A., Gardner D.E., Collier A.M., Clyde W.A.JR 1982; Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science 216:313–315
    [Google Scholar]
  8. Jacobs E., Clad A. 1986; Electroelution of fixed and stained membrane proteins from preparative sodium dodecyl sulfate-polyacrylamide gels into a membrane trap. Analytical Biochemistry 154:583–589
    [Google Scholar]
  9. Jacobs E., Bennewitz A., Bredt W. 1986; Reaction pattern of human anti-Mycoplasma pneumoniae antibodies in enzyme-linked immunosorbent assays and immunoblotting. Journal of Clinical Microbiology 23:517–522
    [Google Scholar]
  10. Jacobs E., Fuchte K., Bredt W. 1987; Amino acid sequence and antigenicity of the aminoterminus of the 168 kDa adherence protein of Mycoplasma pneumoniae. Journal of General Microbiology 133:2233–2236
    [Google Scholar]
  11. Kist M., Jacobs E., Bredt W. 1982; Release of Mycoplasma pneumoniae substances after phagocytosis by guinea pig alveolar macrophages. Infection and Immunity 36:357–362
    [Google Scholar]
  12. Peterson G.L. 1977; A simplification of the protein assay method of Lowry et al.which is more generally applicable. Analytical Biochemistry 83:346–356
    [Google Scholar]
  13. Razin S. 1985; Mycoplasma adherence. In The Mycoplasmas pp. 161–202 Edited by Razin S., Barile M. F. New York: Academic Press;
    [Google Scholar]
  14. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  15. Wessel D., Flügge U.I. 1984; A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Analytical Biochemistry 138:141–143
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-2-473
Loading
/content/journal/micro/10.1099/00221287-134-2-473
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error