1887

Abstract

A catecholamine siderophore, named aminochelin, produced by iron-limited was purified and tentatively identified as 2,3-dihydroxybenzoylputrescine. This compound was first observed as an ethyl-acetate-insoluble catechol that accounted for 30 to 50% of the total catechol in iron-limited culture supernatant fluids. The purified compound was unstable at neutral to alkaline pH, bound Fe, Fe and molybdate, and promoted Fe-uptake into iron-limited Aminochelin was induced and repressed coordinately with the other catechol siderophore azotochelin. The catechol siderophores were, however, less sensitive to repression by soluble iron than the yellow-green fluorescent peptide siderophore azotobactin.

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/content/journal/micro/10.1099/00221287-134-2-453
1988-02-01
2021-10-25
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References

  1. Arnow L.E. 1937; Colorimetric determination of the components of 3,4-dihydroxyphenylalanine-tyrosine mixtures. Journal of Biological Chemistry 118:531–537
    [Google Scholar]
  2. Collinson S.K., Doran J.L., Page W.J. 1987; Production of 3,4-dihydroxybenzoic acid by Azo- monas macrocytogenes and Azotobacterpaspali. Canadian Journal of Microbiology 33:169–175
    [Google Scholar]
  3. Corbin J.L., Bulen W.A. 1969; The isolation and identification of 2,3-dihydroxybenzoic acid and 2-N,6-N-di-(2,3-dihydroxybenzoyl)-l-lysine formed by iron-deficient Azotobacter vinelandii. Biochemistry 8:757–762
    [Google Scholar]
  4. Csaky T.Z. 1948; On the estimation of bound hydroxylamine in biological materials. Acta chemica scandinavica 2:450–454
    [Google Scholar]
  5. De Lorenzo V., Wee S., Herrero M., Neilands J.B. 1987; Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation {fur) repressor. Journal of Bacteriology 169:2624–2630
    [Google Scholar]
  6. Griffiths G.L., Sigel S.P., Payne S.M., Neilands J.B. 1984; Vibriobactin, a siderophore from Vibrio cholerae. Journal of Biological Chemistry 259:383–385
    [Google Scholar]
  7. Hantke K. 1981; Regulation of ferric iron transport in Escherichia coli K-12: isolation of a constitutive mutant. Molecular and General Genetics 182:288–292
    [Google Scholar]
  8. Hantke K. 1984; Cloning of the repressor protein of iron-regulated systems in Escherichia coli K-12. Molecular and General Genetics 197:337–341
    [Google Scholar]
  9. Knosp O., Von Tigerstrom M., Page W.J. 1984; Siderophore-mediated uptake of iron in Azotobacter vinelandii. Journal of Bacteriology 159:341–347
    [Google Scholar]
  10. Krebs K.G., Heusser D., Wimmer H. 1969; Spray reagents. In Thin Layer Chromatography, pp. 854–909 Stahl E. Edited by New York: Springer-Verlag;
    [Google Scholar]
  11. Neilands J.B. 1981; Microbial iron compounds. Annual Review of Biochemistry 50:715–731
    [Google Scholar]
  12. Ong S.A., Peterson T., Neilands J.B. 1979; Agrobactin, a siderophore from Agrobacterium tume- faciens. Journal of Biological Chemistry 254:1860–1865
    [Google Scholar]
  13. Page W.J. 1987; Iron-dependent production of hydroxamate by sodium-dependent Azotobacter chroococcum. Applied and Environmental Microbiology 53:1418–1424
    [Google Scholar]
  14. Page W.J., Grant G.A. 1987; Effect of mineral iron on the development of transformation competence in Azotobacter vinelandii. FEMS Microbiology tetters 41:257–261
    [Google Scholar]
  15. Page W.J., Huyer M. 1984; Derepression of the Azotobacter vinelandii siderophore system, using iron containing minerals to limit iron repletion. Journal of Bacteriology 158:496–501
    [Google Scholar]
  16. Page W.J., Von Tigerstrom M. 1982; Iron- and molybdenum-repressible outer membrane proteins in competent Azotobacter vinelandii. Journal of Bacteriology 151:237–242
    [Google Scholar]
  17. Pittard A.J., Gibson F., Doy C.H. 1961; Phenolic compounds accumulated by washed cell suspensions of a tryptophan auxotroph of Aerobacter aerogenes. Biochimica et biophysica acta 49:485–494
    [Google Scholar]
  18. Post E., Kleiner D., Oelze J. 1983; Whole cell respiration and nitrogenase activities in Azotobacter vinelandii growing in oxygen controlled continuous culture. Archives of Microbiology 134:68–72
    [Google Scholar]
  19. Rennie R.J. 1980; Dinitrogen-fixing bacteria: computer-assisted identification of soil isolates. Canadian Journal of Microbiology 26:1275–1283
    [Google Scholar]
  20. Tait G.T. 1975; The identification and biosynthesis of siderochromes formed by Micrococcus denitrifi- cans. Biochemical Journal 146:191–204
    [Google Scholar]
  21. Thompson J.P., Skerman V.B.D. 1979 Azotobacteriaceae: the Taxonomy and Ecology of the Aerobic Nitrogen-fixing Bacteria. New York: Academic Press;
    [Google Scholar]
  22. Westerveld P., Bloom M.L., Mabbott G.A., Fekete F.A. 1985; The isolation and identification of 3,4-dihydroxybenzoic acid formed by nitrogen-fixing Azomonas macrocytogenes. FEMS Microbiology tetters 30:331–335
    [Google Scholar]
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