1887

Abstract

The gene of , which encodes the transcriptional activator of gene expression, was cloned into a number of plasmid vectors to obtain high-level synthesis of product (NifA). When over-produced, NifA was very insoluble and it precipitated with the cell debris after cell lysis. Localization of -galactosidase activity from a translational fusion confirmed the insoluble nature of NifA. Analysis of two translational fusions in which the last six C-terminal amino acids of NifA were deleted suggests that these residues are required for activity.

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1988-02-01
2024-04-18
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