1887

Abstract

SUMMARY: The gene of , which encodes the transcriptional activator of gene expression, was cloned into a number of plasmid vectors to obtain high-level synthesis of product (NifA). When over-produced, NifA was very insoluble and it precipitated with the cell debris after cell lysis. Localization of β-galactosidase activity from a translational fusion confirmed the insoluble nature of NifA. Analysis of two translational fusions in which the last six C-terminal amino acids of NifA were deleted suggests that these residues are required for activity.

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/content/journal/micro/10.1099/00221287-134-2-425
1988-02-01
2019-10-22
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-2-425
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