1887

Abstract

We have screened strains of for spontaneous mutants showing constitutive transfer of the nopaline Ti plasmid pTiC58 during conjugation. The Ti plasmid derivatives obtained could be transferred not only to but also to cells. The Ti plasmid cannot survive as a freely replicating plasmid in , but it can occasionally integrate into the chromosome. However, insertion in tandem of plasmids carrying fd replication origins (pfd plasmids) into the T-DNA provides an indicator for all transfer events into cells, providing fd gene 2 protein is present in these cells. This viral protein causes the excision of one copy of the pfd plasmid and allows its propagation in the host cell. By using this specially designed Ti plasmid, which was also made constitutive in transfer functions, we found plasmid exchange among strains and between and cells to be equally efficient. A Ti plasmid with repressed transfer functions was transferred to with a rate similar to the low frequency at which it was transferred to The expression of transfer functions of plasmid RP4 either in or in did not increase the transfer of the Ti plasmid into cells, nor did the addition of acetosyringone, an inducer of T-DNA transfer to plant cells. The results show that can transfer the Ti plasmid to with the same efficiency as within its own species. Conjugational transmission of extrachromosomal DNA like the narrow-host-range Ti plasmid may often not only occur among partners allowing propagation of the plasmid, but also on a ‘try-all’ basis including hosts which do not replicate the transferred DNA.

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1988-02-01
2021-07-28
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References

  1. Arthur H. M., Bramhill D., Eastlake P. B., Emmerson P. T. 1982; Cloning of the uvrD gene of E. coli and identification of the product. Gene 19:285–295
    [Google Scholar]
  2. Bevan M. 1984; Binary Agrobacterium vectors for plant transformation. Nucleic Acids Research 12:8711–8721
    [Google Scholar]
  3. Binns A. N., Sciaky D., Wood H. N. 1982; Variation in hormone autonomy and regenerative potential of cells transformed by strain A66 of Agrobacterium tumefaciens. Cell 31:605–612
    [Google Scholar]
  4. Boulnois G. J., Varley J. M., Sharpe G. S., Franklin F. C. H. 1985; Transposon donor plasmids, based on ColIb-P9, for use in Pseudomonas putida and a variety of other Gram-negative bacteria. Molecular and General Genetics 200:65–67
    [Google Scholar]
  5. Chilton M. -D., Ferrand S. K., Levin R., Nester E. W. 1976; RP4 promotion of transfer of a large Agrobacterium plasmid which confers virulence. Genetics 83:609–618
    [Google Scholar]
  6. Cooksey D. A. 1986; A spontaneous insertion in the agrocin sensitivity region of the Ti-plasmid of Agrobacterium tumefaciens C58. Plasmid 16:222–224
    [Google Scholar]
  7. Dagert M., Ehrlich S. D. 1979; Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6:23–28
    [Google Scholar]
  8. Ditta G., Schmidhauser T., Yakobson E., Lu P., Liang X. -W., Finlay D. R., Guiney D., Helsinski D. R. 1985; Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression. Plasmid 13:149–153
    [Google Scholar]
  9. Dotto G. P., Horiuchi K. 1981; Replication of a plasmid containing two origins of bacteriophage f 1. Journal of Molecular Biology 153:169–176
    [Google Scholar]
  10. Ellis J. G., Kerr A., Petit A., Tempé J. 1982a; Conjugal transfer of nopaline and agropine Ti- plasmids - the role of agrocinopines. Molecular and General Genetics 186:269–274
    [Google Scholar]
  11. Ellis J. G., Murphy P. J., Kerr A. 1982b; Isolation and properties of transfer regulatory mutants of the nopaline Ti-plasmid pTiC58. Molecular and General Genetics 186:275–281
    [Google Scholar]
  12. Fraley R. T., Rogers S. G., Horsch R. B., Sanders P. R., Flick J. S., Adams S. P., Bittner M. L., Brand L. A., Fink C. L., Fry J. S., Galluppi G. R., Goldberg S. B., Hoffmann N. L., Woo S. C. 1983; Expression of bacterial genes in plants. Proceedings of the National Academy of Sciences of the United States of America 80:4803–4807
    [Google Scholar]
  13. Fraley R. T., Rogers S. G., Horsch R. B., Eichholtz D. A., Flick J. S., Fink C. L., Hoffmann N. L., Sanders P. R. 1985; The SEV system: a new disarmed Ti plasmid vector system for plant transformation. Biotechnology 3:629–635
    [Google Scholar]
  14. Geider K. 1986; DNA cloning vectors utilizing replication functions of the filamentous phages of Escherichia coli. Journal of General Virology 67:2287–2303
    [Google Scholar]
  15. Geider K., Hohmeyer C., Haas R., Meyer T. F. 1985; A plasmid cloning system utilizing replication and packaging functions of the filamentous bacteriophage fd. Gene 33:341–348
    [Google Scholar]
  16. Genetello C., Van Larebeke N., Holsters M., Depicker A., Van Montagu M., Schell J. 1977; Ti plasmids of Agrobacterium as conjugative plasmids. Nature; London: 265561–563
    [Google Scholar]
  17. Gheysen G., Dhaese P., Van Montagu M., Schell J. 1985; DNA flux across genetic barriers: the crown gall phenomenon. In Advances in Plant Gene Research 2 pp. Hohn B., Dennis E. S. Edited by Wien: Springer Verlag;
    [Google Scholar]
  18. Guiney D. G., Chikami G., Deiss C., Yakobson E. 1985; The origin of plasmid DNA transfer during bacterial conjugation. In Plasmids in Bacteria pp. 521–534 Helinski D. R., Cohen S. N., Clewell D. B., Jackson D. A., Hollaender A. Edited by New York & London: Plenum Press;
    [Google Scholar]
  19. Hamilton R. H., Fall M. Z. 1971; The loss of tumor-initiating ability in Agrobacterium tumefaciens by incubation at high temperature. Experientia 27:229–230
    [Google Scholar]
  20. Hendrix R. W., Roberts J. W., Stahl F. W., Weisberg R. A. 1983 Lambda II Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Hille J., Van Kan J., Klasen I., Schilperoort R. 1983; Site-directed mutagenesis in Escherichia coli of stable R772::Ti cointegrate plasmid from Agrobacterium tumefaciens. Journal of Bacteriology 154:693–701
    [Google Scholar]
  22. Hoekema A., Hirsch P. R., Hooykaas P. J. J., Schilperoort R. A. 1983; A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature; London: 303179–180
    [Google Scholar]
  23. Hogrefe C., Friedrich B. 1984; Isolation and characterization of megaplasmid DNA from lithoautotrophic bacteria. Plasmid 12:161–169
    [Google Scholar]
  24. Holmes D. S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
    [Google Scholar]
  25. Holsters M., Silva B., Genetello C., Engler G., Van Vliet F., De Block M., Villaroel R., Van Montagu M., Schell J. 1978; Spontaneous formation of cointegrates of the oncogenic Ti- plasmid and the wide-host-range P-plasmid RP4. Plasmid 1:456–467
    [Google Scholar]
  26. Holsters M., Silva B., Van Vliet F., Genetello C., De Block M., Dhaese P., Depicker A., Inzé D., Engler G., Villaroel R., Van Montagu M., Schell J. 1980; The functional organization of the nopaline A. tumefaciens plasmid pTiC58. Plasmid 3:212–230
    [Google Scholar]
  27. Hooykaas P. J. J., Schilperoort R. A. 1984; The molecular genetics of crown gall tumorigenesis. Advances in Genetics 22:209–283
    [Google Scholar]
  28. Hooykaas P. J. J., Schilperoort R. A. 1985; The Ti-plasmid of Agrobacterium tumefaciens : a natural genetic engineer. Trends in Biochemical Sciences 10:307–309
    [Google Scholar]
  29. Hooykaas P. J. J., Klapwijk P. M., Nuti M. P., Schilperoort R. A., Rörsch A. 1977; Transfer of the Agrobacterium tumefaciens Ti plasmid to avirulent agrobacteria and to rhizobium ex planta. Journal of General Microbiology 98:477–484
    [Google Scholar]
  30. Kerr A. 1969; Transfer of virulence between isolates of Agrobacterium. Nature; London: 2231175–1176
    [Google Scholar]
  31. Kerr A., Manigault P., Tempé J. 1977; Transfer of virulence in vivo in Agrobacterium. Nature; London: 265560–561
    [Google Scholar]
  32. Klapwijk P. M., Scheulderman T., Schilperoort R. A. 1978; Coordinated regulation of octopine degradation and conjugative transfer of Ti- plasmids in Agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons. Journal of Bacteriology 136:775–785
    [Google Scholar]
  33. Koekman B. P., Ooms G., Klapwijk P. M., Schilperoort R. A. 1979; Genetic map of an octopine Ti-plasmid. Plasmid 2:347–357
    [Google Scholar]
  34. Machida Y., Sakurai M., Kiyokawa S., Ubasawa A., Suzuki Y., Ikeda J. -E. 1984; Nucleotide sequence of the insertion sequence found in the T- DNA region of mutant Ti plasmid pTiA66 and distribution of its homologues in octopine Ti plasmids. Proceedings of the National Academy of Sciences of the United States of America 81:7495–7499
    [Google Scholar]
  35. Martin R. R., Thorlton C. L., Unger L. 1981; Formation of Escherichia coli Hfr strains by integrative suppression with the P group plasmid RP1. Journal of Bacteriology 145:713–721
    [Google Scholar]
  36. Meyer T. F., Geider K. 1979; Bacteriophage fd gene II-protein. II. Specific cleavage and relaxation of supercoiled RF from filamentous phages. Journal of Biological Chemistry 254:12642–12646
    [Google Scholar]
  37. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  38. Moody E. E. M., Runge R. 1972; The integration of autonomous transmissible plasmids into the chromosome of Escherichia coli K12. Genetical Research 19:181–186
    [Google Scholar]
  39. Nester E. W., Gordon M. P., Amasina R. M., Yanofsky M. F. 1984; Crown gall: a molecular and physiological analysis. Annual Review of Plant Physiology 35:387–413
    [Google Scholar]
  40. Petit A., Tempé J. 1978; Isolation of Agrobacterium Ti-plasmid regulatory mutants. Molecular and General Genetics 167:147–155
    [Google Scholar]
  41. Petit A., Tempé J., Kerr A., Holsters M., Van Montagu M., Schell J. 1978; Substrate induction of conjugative activity of Agrobacterium tumefaciens Ti-plasmids. Nature; London: 271570–571
    [Google Scholar]
  42. Ray D. S., Hines J. C., Kim M. H., Imber R., Nomura N. 1982; M13 vectors for selective cloning of sequences specifying initiation of DNA synthesis on single-stranded templates. Gene 18:231–238
    [Google Scholar]
  43. Schweitzer S., Blohm D., Geider K. 1980; Expression of Ti-plasmid DNA in E. coli: comparison of homologous fragments cloned from Ti plasmids of Agrobacterium strains C58 and Ach5. Plasmid 4:196–204
    [Google Scholar]
  44. Simon R., Priefer U., Pühler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. Biotechnology 1:784–791
    [Google Scholar]
  45. Stachel S. E., Messens E., Van Montagu M., Zambryski P. 1985; Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens. Nature; London: 318624–629
    [Google Scholar]
  46. Van Larebeke N., Engler G., Holsters M., Van Den Elsacker S., Zaenen I., Schilperoort R. A., Schell J. 1974; Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability. Nature; London: 252169–170
    [Google Scholar]
  47. Van Larebeke N., Genetello C., Hernalsteeens J. P., Depicker A., Zaenen I., Messens E., Van Montagu M., Schell J. 1977; Transfer of Ti- plasmids between Agrobacterium strains by mobilisation with the conjugative plasmid RP4. Molecular and General Genetics 152:119–124
    [Google Scholar]
  48. Waldron C., Hepburn A. G. 1983; Extra DNA in the T region of crown gall Ti-plasmid pTi66. Plasmid 10:199–203
    [Google Scholar]
  49. Watson B., Currier T. C., Gordon M. P., Chilton M. -D., Nester E. W. 1975; Plasmid required for virulence of Agrobacterium tumefaciens. Journal of Bacteriology 123:255–264
    [Google Scholar]
  50. Zahm P., Hohmeyer C., Geider K. 1985; Site- specific mutagenesis of the Ti plasmid by transformation of Agrobacterium tumefaciens with mutagen- ized T-DNA fragments cloned in E. coli plasmids. Molecular and General Genetics 194:188–194
    [Google Scholar]
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