Enzymes representative of carbohydrate and nitrogen metabolism were screened for their presence and activity amongst species of the genus Fusobacterium. Glutamate dehydrogenase (GDH) was reliably detected in all 25 strains studied. The pH profile of this enzyme and the DNA base composition of selected strains were also determined. DNA base composition of selected strains ranged between 28-32.9 mol% G + C. GDH was active between pH 7.5-11.5 but two pH profiles of activity, with optima at 9.5 and 10.5, were discernible among species. Apart from Fusobacterium nucleatum, which had a heterogeneous enzyme pattern, the GDH electrophoretic mobility was constant within a species but in a few cases the enzyme bands overlapped. A combination of the pH profile, the GDH electrophoretic pattern and the DNA base composition provided clear separation of the test organisms into discrete groups; however, a larger number of strains must be examined before the full potential of these tests can be evaluated.
CollinsM.D.,
ShahH.N.1987; Recent advances in the chemotaxonomy of the genus Bacteroides.
. In Recent Advances in Anaerobic Bacteriology pp. 249–258 Edited by
BorrielloS. P.,
HardieJ. M.,
DraserB. S.,
DuerdenB. I.,
HudsonM. J.,
LysonsR. J.
Dordrecht, Boston & Lancaster: Martinus Nijhogg;
GharbiaS.E.,
ShahH.N.1986; Biochemical properties of species of the genus Fusobacterium.
. XIV International Congress of Microbiology September 1986, Manchester, UK), abstract P
GoodfellowM.,
MinnikinD.E.1985; Introduction to chemotaxonomy. In Chemical Methods in Bacterial Systematics pp. 1–15 Edited by
GoodfellowM.,
MinnikinD. E.
London: Academic Press;
KatoK.,
UmemdoT.,
FurkuharaH.,
SagawaH.,
KotanS.1981; Variation of dibasic amino acid in the cell wall peptidoglycan of bacteria of the genus Fusobacterium.
. FEMS Microbiology Letters 10:81–86
LoveD.N.,
CatoE.P.,
JohnsonJ.L.,
JonesR.F.,
BaileyM.1987; Deoxyribonucleic acid hybridization among strains of Fusobacterium isolated from soft tissue infections of cats: comparison with human and animal type strains from oral and other sites. International Journal of Systematic Bacteriology 37:23–26
MiyagawaE:,
AzumaR.,
SutoT.1981; Peptidoglycan composition of Gram-negative obligatory anaerobic rods. Journal of General and Applied Microbiology 27:199–208
MooreW.E.C.,
HoldemanL.V.,
KelleyW.1984; Genus Fusobacterium (Knorr, 1922). In Bergey’s Manual of Systematic Bacteriology1 pp. 631–637 Edited by
KriegN. R.
Baltimore: Williams & Wilkins;
ShahH.N.,
WilliamsR.A.D.,
BowdenG.H.,
HardieJ.M.1976; Comparison of the biochemical properties of Bacteroides melaninogenicus from human dental plaque and other sites. Journal of Applied Bacteriology 41:473–492
ShahH.N.,
GharbiaS. E.,
Al-JaliliT. A. R.,
SeddonS. V.,
NashR. A.1987a; Enzymes of diagnostic importance within the Bacteroidaceae, use as possible ecological markers. Microbial Ecology in Health and Disease in the Press
ShahH.N.,
NashR. A.,
GharbiaS. E.1987b; Bacteroides and fusobacteria. In Anaerobes and Human Diseases Edited by
DuerdenB. I.,
DraserB. S.
London: Edward Arnold; in the Press
VasstrandE.N.,
HofstadT.,
EndrsenC.,
JansenH.B.1979; Demonstration of lanthionine as a natural constituent of the peptidoglycan of Fusobacterium nucleatum FeV 1. Infection and Immunity 25:775–780
VasstrandE.N.,
JansenH.B.,
MironT.,
HofstadT.1982; Composition of peptidoglycan in Bacteroidaceae: determination and distribution of lanthionine. Infection and Immunity 36:114–122
WatabeJ.,
BennoY.,
MitsuokaT.1983; Taxonomic study of Bacteroides oralis and related organisms and proposal of Bacteroides veroralis sp.nov. International Journal of Systematic Bacteriology 33:57–64
WilliamsR.A.D.,
ShahH.N.1980; Enzyme patterns in bacterial classification and identification. In Microbiological Classification and Identification pp. 299–315 Edited by
GoodfellowM.,
BoardR. G.
London: Academic Press;