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1887

Abstract

Techniques were developed for the rapid and simple electron microscopic visualization of isolated cyanophycin granules to allow analysis of their size from cells grown under different conditions. Granules were purified by differential and renografin density-gradient centrifugation from cells grown for various times in chloramphenicol-containing media, or in nitrogen-limited media to which nitrogen was then added back, or under light limitation. Granules were then observed by electron microscopy after staining with uranyl acetate, and random granule diameters were measured. Granule size increased significantly with time following chloramphenicol treatment and following light limitation. This suggests that much of the increase in amount of cyanophycin in cells treated with chloramphenicol or light limitation is caused by increase in granule size rather than in granule number. Insignificant changes in granule size were observed during nitrogen repletion, suggesting increase in granule number, not size.

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/content/journal/micro/10.1099/00221287-134-2-295
1988-02-01
2025-01-22
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References

  1. Allen M.M. 1968; Simple conditions for growth of unicellular blue-green algae on plates. Journal of Phycology 4:1–4
    [Google Scholar]
  2. Allen M.M., Hawley M.A. 1983; Protein degradation and synthesis of cyanophycin granule polypeptide in Aphanocapsa sp. Journal of Bacteriology 154:1480–1484
    [Google Scholar]
  3. Allen M.M., Hutchison F. 1980; Nitrogen limitation and recovery in the cyanobacterium Aphanocapsa 6308. Archives of Microbiology 128:1–7
    [Google Scholar]
  4. Allen M.M., Weathers P.J. 1980; Structure and composition of cyanophycin granules in the cyanobacterium Aphanocapsa 6308. Journal of Bacteriology 141:959–962
    [Google Scholar]
  5. Allen M.M., Hutchison F., Weathers P.J. 1980; Cyanophycin granule polypeptide formation and degradation in the cyanobacterium Aphanocapsa 6308. Journal of Bacteriology 141:687–693
    [Google Scholar]
  6. Bruning J.L., Kintz B.L. 1968 Computational Handbook of Statistics. Glenview, Illinois: Scott Foresman & Company;
    [Google Scholar]
  7. Lang N.L, Simon R.D., Wolk C.P. 1972; Correspondence of cyanophycin granules with structural granules in Anabaena cylindrica. . Archiv für Mikrobiologie 83:313–320
    [Google Scholar]
  8. Lawry N.H., Simon R.D. 1982; The normal and induced occurrence of cyanophycin inclusion bodies in several blue-green algae. Journal of Phycology 18:391–399
    [Google Scholar]
  9. Simon R.D. 1973a; The effect of chloramphenicol on the production of cyanophycin granule polypeptide in the blue-green alga Anabaena cylindrica. . Archiv für Mikrobiologie 92:115–122
    [Google Scholar]
  10. Simon R.D. 1973b; Measurement of the cyanophycin granule polypeptide content in the blue-green alga Anabaena cylindrica. . Journal of Bacteriology 115:1213–1216
    [Google Scholar]
  11. Tahmida khan Z.N., Godward M.B.E. 1978; Cyanophycin granules in a blue-green algae Calo-thrix marchica Lemm. Current Science 47:710–712
    [Google Scholar]
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