1887

Abstract

When cells of were incubated under fasting conditions and then plated in the presence of an inhibitor of protein synthesis, a variable but significant (>10) fraction of the population changed their morphology from rod to sphere, with a considerable thickening of the cell wall. This change was accompanied by metabolic and antibiotic-resistance modifications, including the synthesis of at least one new enzyme (α-glucosidase), and by the simultaneous appearance of several new species of DNA, presumably plasmids. The round cells grew faster than the parent strain and maintained their morphology indefinitely when propagated on complex medium containing glucose as the main carbon source. However, when glucose was omitted, cells returned to the rod form and regained their previous characteristics, including the absence of detectable plasmids.

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1988-02-01
2024-04-19
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References

  1. Boylan R.J., Mendelson N.H. 1969; Initial characterization of a temperature sensitive rod mutant of Bacillus subtilis. Journal of Bacteriology 100:1316–1321
    [Google Scholar]
  2. Clark J.B. 1972; Morphogenesis in the genus Arthrobacter. Critical Reviews in Microbiology 1:521–544
    [Google Scholar]
  3. Dills S.S., Apperson A., Schmidt M.R., Saier M.H. JR 1980; Carbohydrate transport in bacteria. Microbiological Reviews 44:385–418
    [Google Scholar]
  4. Ensign J.C., Wolfe R.S. 1964; Nutritional control of morphogenesis in Arthrobacter crystallo-poietes. Journal of Bacteriology 87:924–932
    [Google Scholar]
  5. Fujiwara T., Fukui S. 1972; Isolation of morphological mutants of Agrobacterium tume-faciens. Journal of Bacteriology 110:743–746
    [Google Scholar]
  6. Hancock R., Park J.T. 1958; Cell-wall synthesis by Staphylococcus aureus in the presence of chloram-phenicol. Nature; London: 1811050–1052
    [Google Scholar]
  7. Hettinga D.H., Reinbold G.W. 1972; The propionic acid bacteria. A review. Journal of Milk and Food Technology 35:295–301
    [Google Scholar]
  8. Higgins M.L., Shockman G.D. 1970; Early changes in the ultrastructure of Streptococcus faecalis after amino acid starvation. Journal of Bacteriology 103:244–254
    [Google Scholar]
  9. Kumar S. 1976; Properties of adenyl cyclase and cyclic adenosine 3’,5’-monophosphate receptor protein-deficient mutants of Escherichia coli. Journal of Bactriology 125:545–555
    [Google Scholar]
  10. Matsuzawa H., Hayakawa K., Sato T., Imahori K. 1973; Characterization and genetic analysis of a mutant of Escherichia coli K12 with rounded morphology. Journal of Bacteriology 115:436–442
    [Google Scholar]
  11. Molinari R., Lara F.J.S. 1960; The lactic dehydrogenase of Propionibacterium pentosaceum. Biochemical Journal 75:57–65
    [Google Scholar]
  12. Moore W.E.C., Holdeman L.V. 1974; Propionibacterium. In Bergey’s Manual of Determinative Bacteriology, 8th edn. pp. 633–641 Buchanan R.E., Gibbons N.E. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  13. Rogers H.J., Mcconnell M., Burdett I.D.J. 1970; The role of l-glutamine in the phenotypic change of a rod mutant derived from Bacillus subtilis 168. Journal of General Microbiology 61:173–141
    [Google Scholar]
  14. Rogers H.J., Mcconnell M., Burdett R.C. 1968; Cell wall or membrane mutants of Bacillus subtilis and Bacillus licheniformis with grossly deformed morphology. Nature; London: 219285–288
    [Google Scholar]
  15. Rogers H.J., Mcconnell M., Burdett I.D.J. 1970; The isolation and characterization of mutants of Bacillus subtilis and Bacillus licheniformis with disturbed morphology and cell division. Journal of General Microbiology 61:155–171
    [Google Scholar]
  16. Rogers H.J., Mcconnell M., Hughes R.C. 1971; The chemistry of the cell walls of rod mutants of Bacillus subtilis. Journal of General Microbiology 66:297–308
    [Google Scholar]
  17. Rogers H.J., Thurman P.F., Buxton R.S. The chemistry of the cell walls of rod mutants of Bacillus subtilis. Journal of General Microbiology 66:297–308
    [Google Scholar]
  18. Santamaria R., Gil J.A., Mesas J.M., Martin J.F. 1984; Characterization of an endogenous plasmid and development of cloning vectors and a transformation system in Brevibacterium lactofermentum. Journal of General Microbiology 130:2237–2246
    [Google Scholar]
  19. Satta G., Botta G., Canepari P., Fontana R. 1981; Early initiation of deoxyribonucleic acid replication and shortening of generation time associated with inhibition of lateral wall formation by mecillinam. Journal of Bacteriology 148:10–19
    [Google Scholar]
  20. Snoswell A.M. 1966; DL-Lactate dehydrogenases (NAD-independent) from Lactobacillus arabinosus. Methods in Enzymology 9:321–327
    [Google Scholar]
  21. Von Freudenreich E., Orla-Jensen S. 1906; Uber die Emmentalerkase stattfindende Propion-saiiregarung. Zentra/blatt fur Bakteriologie, Parasi-tenkunde, Infektions und Hygiene (Abteilung II) 17:529
    [Google Scholar]
  22. Westling-Haggstrom B., Normark S. 1975; Genetic and physiological analysis of an envB spherelike mutant of Escherichia coli K12 and characterization of its transductants. Journal of Bacteriology 123:75–82
    [Google Scholar]
  23. Whitehouse R.S., Benichou J.C., Ryter A. 1977; Procedure for the longitudinal orientation of rod shaped bacteria and the production of a high cell density of procaryotic and eucaryotic cells in thin sectionfor electron microscopy. Biologie cellulaire 30:155–158
    [Google Scholar]
  24. Yoshihama M., Higashiro K., Rao E.A., Akedo M., Shanabruch W.G., Follettie M.T., Walker G.C., Sinskey A.J. 1985; Cloning vector system for Corynebacterium glutamicum. Journal of Bacteriology 162:591–597
    [Google Scholar]
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