1887

Abstract

A collection of 30 independently isolated strains of was screened for the presence of temperate bacteriophages. Two strains were found to harbour phages. The newly isolated phages differ in several respects from the 43 previously isolated phages for Both phages are polyhedral, approximately 60 nm in diameter, and have no apparent tail structure. They are chloroform sensitive, and plaque formation is inhibited by agar. Both are capable of establishing a stable association with host cells. Twenty-nine of the 30 strains produced diffusible substances that specifically inhibited the growth of other strains or closely related species and that could not be propagated. These substances appear to be bacteriocins, some of which, like bacteriophages, are active only against motile cells, while others inhibit nonmotile as well as motile cells. One of each of these two types of bacteriocins was partially characterized and both were found to be proteinaceous in nature and bactericidal in effect.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-2-253
1988-02-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/2/mic-134-2-253.html?itemId=/content/journal/micro/10.1099/00221287-134-2-253&mimeType=html&fmt=ahah

References

  1. Chang L.-Y.E., Pate J.L. 1981; Nutritional requirements of Cytophaga johnsonae and some of its auxotrophic mutants. Current Microbiology 5:235–240
    [Google Scholar]
  2. Chang L.-Y.E., Pate J.L., Betzig R.J. 1984; Isolation and characterization of nonspreading mutants of the gliding bacterium Cytophaga johnsonae. . Journal of Bacteriology 159:26–35
    [Google Scholar]
  3. Christensen P.J. 1977; The history, biology, and taxonomy of the Cytophaga group. Canadian Journal of Microbiology 23:1599–1653
    [Google Scholar]
  4. Christensen P.J., Cook F.D. 1978; Lysobacter, a new genus of nonfruiting gliding bacteria with a high base ratio. International Journal of Systematic Bacteriology 28:367–393
    [Google Scholar]
  5. Ensign J.C., Wolfe R.S. 1965; Lysis of bacterial cell walls by an enzyme isolated from a myxobacter. Journal of Bacteriology 90:395–402
    [Google Scholar]
  6. Leadbetter E.R. 1974; Part 2, Order II. Cytopha- gales. In Bergey’s Manual of Determinative Bacteriology, 8th edn. pp. 99–127 Edited by Buchanan R. E., Gibbons N. E. Baltimore: Williams & Wilkins;
    [Google Scholar]
  7. Lingappa Y., Lockwood J.L. 1961; A chitin medium for isolation, growth and maintenance of actinomycetes. Nature, London 189:158–159
    [Google Scholar]
  8. Martin S., Sodergren E., Masuda T., Kaiser D. 1978; Systematic isolation of transducing phages for Myxococcus xanthus. . Virology 88:44–53
    [Google Scholar]
  9. Pate J.L., Petzold S.J., Chang L.-Y.E. 1979; Phages for the gliding bacterium Cytophaga john- sonae that infect only motile cells. Current Microbiology 2:257–262
    [Google Scholar]
  10. Rosenberg E., Varon M. 1984; Antibiotics and lytic enzymes. In Myxobacteria, Development and Cell Interactions pp. 109–125 Edited by Rosenberg E. New York: Springer-Verlag;
    [Google Scholar]
  11. Takemoto K.K., Liebhaber H. 1961; Virus- polysaccharide interactionsI. An agar polysaccharide determining plaque morphology of EMC virus. Virology 14:456–462
    [Google Scholar]
  12. Varon M., Cohen S., Rosenberg E. 1984; Autocides produced by Myxococcus xanthus. . Journal of Bacteriology 160:1146–1150
    [Google Scholar]
  13. Varon M., Tietz A., Rosenberg E. 1986; Myxococcus xanthus autocide AML. Journal of Bacteriology 167:356–361
    [Google Scholar]
  14. Wolkin R.H., Pate J.L. 1984; Translocation of motile cells of the gliding bacterium Cytophaga johnsonae depends on a surface component that may be modified by sugars. Journal of General Microbiology 130:2651–2669
    [Google Scholar]
  15. Wolkin R.H., Pate J.L. 1985; Selection for nonadherent or nonhydrophobic mutants co-selects for nonspreading mutants of Cytophaga johnsonae and other gliding bacteria. Journal of General Microbiology 131:737–750
    [Google Scholar]
  16. Wolkin R.H., Pate J.L. 1986; Phage adsorption and cell adherence are motility-dependent characteristics of the gliding bacterium Cytophaga johnsonae. . Journal of General Microbiology 132:355–367
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-2-253
Loading
/content/journal/micro/10.1099/00221287-134-2-253
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error