Pre-incubation of cells of with 2 -ethanol led to decreased rates of -alanine uptake, H efflux and fermentation rate. However, these responses were modified in yeast cells with altered phospholipid composition. Using -alanine transport and H efflux as indices of ethanol tolerance, it was observed that cells enriched with phosphatidylserine had greater tolerance to ethanol. This resulted from altered charge of membrane phospholipids rather than changes in membrane fluidity. It is suggested that the anion:zwitterion ratio of phospholipids may be one of the important determinants of ethanol tolerance in


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