1887

Abstract

The yeast possesses two routes of electron transfer from exogenous NAD(P)H to oxygen. Electrons are transferred either to the classical cytochrome pathway at the level of ubiquinone through an NAD(P)H dehydrogenase, or to an alternative pathway at the level of cytochrome through another NAD(P)H dehydrogenase which is insensitive to antimycin A. Analyses of mitoplasts obtained by digitonin/osmotic shock treatment of mitochondria purified on a sucrose gradient indicated that the NADH and NADPH dehydrogenases serving the alternative route were located on the mitochondrial inner membrane. The dehydrogenases could be differentiated by their pH optima and their sensitivity to amytal, butanedione and mersalyl. No transhydrogenase activity occurred between the dehydrogenases, although NADH oxidation was inhibited by NADP and butanedione. Studies of the effect of NADP on NADH oxidation showed that the NADH:ubiquinone oxidoreductase had Michaelis-Menten kinetics and was inhibited by NADP, whereas the alternative NADH dehydrogenase had allosteric properties (NADH is a negative effector and is displaced from its regulatory site by NAD or NADP).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-12-3195
1988-12-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/12/mic-134-12-3195.html?itemId=/content/journal/micro/10.1099/00221287-134-12-3195&mimeType=html&fmt=ahah

References

  1. Bruinenberg P.M., Van Duken J.P., Kuenen J.G., Scheffers W.A. 1985; Oxidation of NADH and NADPH by mitochondria from the yeast Candida utilis . Journal of General Microbiology 131:1043–1051
    [Google Scholar]
  2. Camougrand N., Caubbt R., Guérin M. 1983; Evidence for an alternative and non-phosphorylat- ing pathway for NADH reoxidation in a yeast resistant to glucose repression. European Journal of Biochemistry 135:367–371
    [Google Scholar]
  3. Camougrand N., Velours G., Guérin M. 1986; The resistance of C. parapstiosis to drugs. Biology of the Cell 58:71–78
    [Google Scholar]
  4. Camougrand N., Mila B., Velours G., Lazowska J., Guérin M. 1988; Discrimination between different groups of Candida parapstiosis by mitochondrial DNA restriction analysis. Current Genetics 13:445–449
    [Google Scholar]
  5. Caubet R., Guérin B., Guérin M. 1988; Comparative studies on the glycolytic and hexose monophosphate pathways in Candida parapstiosis and Saccharomyces cerevisiae . Archives of Microbiology 149:324–329
    [Google Scholar]
  6. De Santis A., Melandri B.A. 1984; The oxidation of external NADH by an intermembrane electron transfer in mitochondria from the ubiquinone-deficient mutant E3-24 of Saccharomyces cerevisiae . Archives of Biochemistry and Biophysics 232:354–365
    [Google Scholar]
  7. Djavadi F.H.S., Moradi M., Djavadi-Ohaniance L. 1980; Direct oxidation of NADPH by submitochondrial particles from S. cerevisiae . European Journal of Biochemistry 107:501–504
    [Google Scholar]
  8. Doussièe J., Sainsard-Chanet A., Vignais P.V. 1979; The respiratory chain of Paramecium tetraur- elia in wild type and the mutant Cll. II. Cyanide insensitive respiration: function and regulation. Biochimica et biophysica acta 548:236–252
    [Google Scholar]
  9. Greenawalt J.W. 1974; The isolation of outer and inner mitochondrial membranes. Methods in Enzymology 31:310–323
    [Google Scholar]
  10. Guérin M., Camougrand N. 1986; The alternative oxidase of C. parapstiosis . European Journal of Biochemistry 159:519–524
    [Google Scholar]
  11. Guérin M., Labbe P., Somlo M. 1979; Preparation of yeast mitochondria (S. cerevisiae) with good P/O and respiratory control ratios. Methods in Enzymology 55:149–159
    [Google Scholar]
  12. Guérin M., Camougrand N., Velours G., Guérin B. 1982; New mutants resistant to glucose repression affected in the regulation of the NADH oxidation. European Journal of Biochemistry 124:457–463
    [Google Scholar]
  13. Guérin M., Camougrand N., Cheyrou A., Henry M.F. 1987; Another type of alternative electron transfer pathway in the yeast Candida parapstiosis . In Plant Mitochondria: Structural, Functional and Physiological Aspects pp 243–246 Moore A. L., Beechey R. B. Edited by New York: Plenum Press;
    [Google Scholar]
  14. Hanssens L., Verachtert H. 1976; Adenosine 5´- monophosphate-stimulated cyanide insensitive respiration in mitochondria of Montiiella tomentosa . Journal of Bacteriology 125:829–836
    [Google Scholar]
  15. Hayaishi O. 1962; Kynurerine hydroxylase. Methods in Enzymology 5:807–809
    [Google Scholar]
  16. Koeppe D.E., Miller J. 1972; Oxidation of reduced nicotinamide adenine dinucleotide phosphate by isolated com mitochondria. Plant Physiology 49:353–357
    [Google Scholar]
  17. Klingenberg M., Durand R., Guérin B. 1974; Analysis of the reactivity of SH-reagents with the mitochondrial phosphate carrier. European Journal of Biochemistry 42:135–150
    [Google Scholar]
  18. Massey V. 1955; Fumarase. Methods in Enzymology 1:729–733
    [Google Scholar]
  19. Møller I.M., Palmer J.P. 1981a; Properties of the oxidation of exogenous NADH and NADPH by plant mitochondria. Evidence against a phosphatase or a mitochondria nucleotide transhydrogenase being responsible for NADPH oxidation. Biochimica et biophysica acta 638:225–233
    [Google Scholar]
  20. Møller I.M., Palmer J.P. 1981b; The inhibition of exogenous NAD(P)H oxidation in plant mitochondria by chelators and mersalyl as a function of pH. Physiologia plantarum 53:413–420
    [Google Scholar]
  21. Møller I.M., Schwitzgubbel J.P., Palmer J.M. 1982; Binding and screening by cations and the effect on exogenous NAD(P)H oxidation in Neurospora crassa mitochondria. European Journal of Biochemistry 123:81–88
    [Google Scholar]
  22. Ohnishi T. 1970; Induction of site I phosphorylation in vivo in Saccharomyces carlsbergensis . Biochemical and Biophysical Research Communications 41:344–352
    [Google Scholar]
  23. Ohnishi T., Sottocasa G., Ernster L. 1966; Current approaches to the mechanism of energycoupling in the respiratory chain. Studies with yeast mitochondria. Bulletin de la Societe de chimie biologique 48:1189–1203
    [Google Scholar]
  24. Palmer J.M., Møller I.M. 1982; Regulation of NAD(P)H dehydrogenase in plant mitochondria. Trends in Biochemical Sciences 7:258–261
    [Google Scholar]
  25. Phelps D.C., Hatefi Y. 1984; Interaction of purified nicotinamide nucleotide transhydrogenase with dicyclohexylcarbodiimide. Biochemistry 23:4475–4480
    [Google Scholar]
  26. Riordan J.F. 1973; Functional arginyl residues in carboxypeptidase A. Modification with butane- dione. Biochemistry 12:3915–3923
    [Google Scholar]
  27. Rydstrom J. 1979; Assay of nicotinamide nucleotide transhydrogenases in mammalian, bacterial and reconstituted systems. Methods in Enzymology 55:261–275
    [Google Scholar]
  28. Schnaitman C.A., Greenawalt J.W. 1968; Enzymatic properties of the inner and outer membranes of rat liver mitochondria. Journal of Cellular Biology 38:158–175
    [Google Scholar]
  29. Schuurmans-Stekhoven F.M.A.H. 1966; Studies on yeast mitochondria. I. Existence of three phosphorylation sites along the respiratory chain of yeast mitochondria. Archives of Biochemistry and Biophysics 115:555–568
    [Google Scholar]
  30. Schwitzguebel J.P., Palmer J.M. 1981; Properties of mitochondria isolated from Neurospora crassa grown with acetate. FEMS Microbiology Utters 11:273–277
    [Google Scholar]
  31. Sotthibandhu R., Palmer J.M. 1975; The activation of non-phosphorylating electron transport by adenine nucleotides in Jerusalem artichoke (Helianthus tuberosus) mitochondria. Biochemical Journal 152:637–645
    [Google Scholar]
  32. Vanderleyden J., Kurth I., Verachtert H. 1979; Characterization of cyanide-insensitive respiration in mitochondria and submitochondrial particles in Moniliella tomentosa . Biochemical Journal 182:437–443
    [Google Scholar]
  33. Vonjagow G., Klingenberg M. 1970; Pathways of hydrogen in mitochondria of S. carlsbergensis . European Journal of Biochemistry 12:583–592
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-12-3195
Loading
/content/journal/micro/10.1099/00221287-134-12-3195
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error