Bacillus stearothermophilus IC3 degraded the meta cleavage product of catechol, 2-hydroxymuconic semialdehyde, to pyruvate and acetaldehyde via the 4-oxalocrotonate pathway. The pathway was identical to those previously delineated in several mesophilic organisms. However, all the enzymes showed activity at 55 °C and other properties (substrate specificities and effects of metal ions) also differed from those displayed by the mesophilic enzymes. All enzymes of this meta cleavage pathway, except the 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase and 4-hydroxy-2-oxovalerate aldolase activities, were induced by growth on phenol.
BarbourM.G.,
BaylyR.C.1980; Mutants defective in isomerase and decarboxylase activities of the 4-hydroxyphenylacetic acid meta cleavage pathway in Pseudomonas putida
. Journal of Bacteriology 142:480–485
BaylyR.C.,
Di BerardinoD.1978; Purification and properties of 2-hydroxy-6-oxo-2,4-heptadi- enoate hydrolase from two strains of Pseudomonas putida
. Journal of Bacteriology 134:30–37
BaylyR.C.,
Di BerardinoD.,
HughesE.J.L.,
SkurrayR.A.1987; The purification and properties of two isofunctional 2-hydroxy-6-oxo- hepta-2,4-dienoate hydrolases from Alcaligenes eutrophus strain 345. Journal of General Microbiology 133:815–822
BradfordM.M.1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
BrownJ.P.,
PerhamR.N.1976; Selective inactivation of the transacylase components of the 2- oxo acid dehydrogenase multienzyme complex of Escherichia coli
. Biochemical Journal 155:419–427
CollinsworthW.L.,
ChapmanP.J.,
DagleyS.1973; Stereospecific enzymes in the degradation of aromatic compounds by Pseudomonas putida
. Journal of Bacteriology 133:922–931
DegryseE.,
GlansdorfN.,
PiÉrardA.1978; A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus
. Archives of Microbiology 117:189–196
DugglebyC.J.,
WilliamsP.A.1986; Purification and some properties of the 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase (2-hydroxymuconic semialdehyde hydrolase) encoded by the TOL plasmid pWWO from Pseudomonas putida mt-2. Journal of General Microbiology 132:717–726
GolovachevaR.S.,
OreshkinA.E.1975; Oxidation of phenol by certain strains of Bacillus stearothermophilus
. Microbiology (USSR) 44:470–475 (English translation. New York: Plenum Press.)
HarayamaS.,
MermodN.,
RekikM.,
LehrbachP.R.,
TimmisK.N.1987; Roles of the divergent branches of the mem-cleavage pathways in the degradation of benzoate and substituted benzoates. Journal of Bacteriology 169:558–564
LampelK.A.1979The p-cymene pathway in Pseudomonas putida: a comparative study of the three enzymes catalysing sequential reactions subsequent to ring cleavage. PhD dissertation University of Miami, USA.:
MurrayK.,
DugglebyC.J.,
Sala-TrepatJ.M.,
WilliamsP.A.1972; The metabolism of benzoate and methylbenzoatesvia the meta cleavage pathway by Pseudomonas arvilla mt-2. European Journal of Biochemistry 28:301–310
Sala-TrepatJ.M.,
EvansW.C.1971b; The metabolism of 2-hydroxymuconic semialdehyde by Azotobacter species. Biochemical and Biophysical Research Communications 43:456–462
WigmoreG.J.,
BaylyR.C.,
DiberardinoD.1974; Pseudomonas putida mutants defective in the metabolism of the products of meta fission of catechol and its methyl analogues. Journal of Bacteriology 120:31–37